##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AccI AclI AflII AflIII AgeI
AhdI ApaI AscI AseI AsnI
AspEI AviII BciVI BclI BfrI
BsiWI Bsp1407I BspHI BspLU11I BsrGI
BssHII BssSI Bst1107I BstBI BstZ17I
CpoI EagI Eam1105I EclXI Eco52I
EcoT22I FbaI FseI FspI HincII
HindII HpaI KspI MfeI MluI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 2090 GACGTC GACGT^C
Acc65I 3539 GGTACC G^GTACC
AccIII 753 TCCGGA T^CCGGA
Alw44I 1674 GTGCAC G^TGCAC
Aor51HI 398 AGCGCT AGC^GCT
ApaLI 1674 GTGCAC G^TGCAC
Asp718I 3539 GGTACC G^GTACC
AspI 4160 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4143 CCTAGG C^CTAGG
BbeI 3367 GGCGCC GGCGC^C
BbrPI 228 CACGTG CAC^GTG
BcgI 3908 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 3448 AGATCT A^GATCT
BlnI 4143 CCTAGG C^CTAGG
BlpI 2408 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2408 GCTGAGC GC^T[ACGT]AGC
BsaBI 831 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 753 TCCGGA [AT]^CCGG[AT]
BseAI 753 TCCGGA T^CCGGA
BsiEI 119 CGACCG CG[GA][CT]^CG
BsmBI 610 CGTCTC CGTCTC(1/5)
BspDI 959 ATCGAT AT^CGAT
BspEI 753 TCCGGA T^CCGGA
BspMI 2145 ACCTGC ACCTGC(4/8)
BsrDI 1438 CATTGC GCAATG(2/0)
CelII 2408 GCTGAGC GC^T[ACGT]AGC
ClaI 959 ATCGAT AT^CGAT
DrdI 2934 GACTCCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 398 AGCGCT AGC^GCT
EcoRI 1006 GAATTC G^AATTC
KasI 3367 GGCGCC G^GCGCC
KpnI 3539 GGTACC GGTAC^C
MamI 831 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 753 TCCGGA T^CCGGA
NarI 3367 GGCGCC GG^CGCC
NcoI 2430 CCATGG C^CATGG
PflFI 4160 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 228 CACGTG CAC^GTG
PmlI 228 CACGTG CAC^GTG
PshAI 2683 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 3282 CTGCAG CTGCA^G
SacI 3426 GAGCTC GAGCT^C
SfoI 3367 GGCGCC GGC^GCC
SmaI 995 CCCGGG CCC^GGG
SphI 4040 GCATGC GCATG^C
SspI 1183 AATATT AAT^ATT
Tth111I 4160 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 632 TCTAGA T^CTAGA
XmaCI 995 CCCGGG C^CCGGG
XmaI 995 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
Asp700I 1147 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1318 GAACCACTTC
AvaI 571 CCCGAG C^[CT]CG[GA]G
995 CCCGGG
BamHI 744 GGATCC G^GATCC
2588 GGATCC
BglI 1230 GCCGATATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1794 GCCGGGAAGGC
BsaAI 228 CACGTG [CT]AC^GT[GA]
2452 TACGTG
BsmI 699 GAATGC GAATGC(1/-1)
1558 GAATGC
BsoBI 571 CCCGAG C^[CT]CG[GA]G
995 CCCGGG
DraI 3488 TTTAAA TTT^AAA
3568 TTTAAA
DsaI 1927 CCACGG C^C[GA][CT]GG
2430 CCATGG
EcoNI 300 CCTTTGTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3027 CCTATCTGAGG
EcoRV 1775 GATATC GAT^ATC
3213 GATATC
MslI 1544 CATGAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1688 CACCAGGATG
NspI 2190 ACATGC [GA]CATG^[CT]
4040 GCATGC
ScaI 1465 AGTACT AGT^ACT
2175 AGTACT
XcmI 1040 CCACAGGGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2010 CCACTGGCAAAGTGG
XmnI 1147 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1318 GAACCACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
AspHI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrBI
BsrFI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
EaeI EarI Eco81I EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV PflMI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII