##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
Acc65I AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AseI AsnI
Asp718I AspEI AvrII BamHI BciVI
BfrI BglII BlnI BsiWI BsmI
Bsp1407I BspDI BspLU11I BsrDI BsrGI
Bst1107I BstZ17I Bsu36I ClaI CpoI
DraI DrdI Eam1105I Eco47III Eco81I
EcoRV EcoT22I FseI KpnI MfeI
MluI MunI NheI NruI NsiI
PacI PaeR7I PinAI PmeI PshBI
PvuI RsrII SbfI ScaI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 3597 GACGTC GACGT^C
AccIII 3016 TCCGGA T^CCGGA
AclI 5397 AACGTT AA^CGTT
AcsI 698 GAATTC [GA]^AATT[CT]
ApoI 698 GAATTC [GA]^AATT[CT]
AscI 4768 GGCGCGCC GG^CGCGCC
Asp700I 3544 GAATCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 3463 TGCGCA TGC^GCA
BbrPI 2982 CACGTG CAC^GTG
BclI 479 TGATCA T^GATCA
BlpI 3167 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3167 GCTCAGC GC^T[ACGT]AGC
BsaBI 480 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3016 TCCGGA T^CCGGA
BspEI 3016 TCCGGA T^CCGGA
BspHI 482 TCATGA T^CATGA
BstBI 719 TTCGAA TT^CGAA
BstEII 2751 GGTGACC G^GT[ACGT]ACC
BstPI 2751 GGTGACC G^GT[ACGT]ACC
CelII 3167 GCTCAGC GC^T[ACGT]AGC
DraIII 3923 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 2751 GGTGACC G^GT[ACGT]ACC
EcoRI 698 GAATTC G^AATTC
FbaI 479 TGATCA T^GATCA
FspI 3463 TGCGCA TGC^GCA
HindIII 3153 AAGCTT A^AGCTT
HpaI 4751 GTTAAC GTT^AAC
MamI 480 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3016 TCCGGA T^CCGGA
MslI 2942 CACGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 337 CATATG CA^TATG
NotI 4305 GCGGCCGC GC^GGCCGC
NspV 719 TTCGAA TT^CGAA
PflMI 801 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2982 CACGTG CAC^GTG
PmlI 2982 CACGTG CAC^GTG
PshAI 2630 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 5397 AACGTT AA^CGTT
RcaI 482 TCATGA T^CATGA
SalI 311 GTCGAC G^TCGAC
SexAI 4252 ACCAGGT A^CC[AT]GGT
SfuI 719 TTCGAA TT^CGAA
SmlI 3642 CTCAAG C^T[CT][GA]AG
Van91I 801 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 3544 GAATCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AfaI 4067 GTAC GT^AC
5019 GTAC
AflIII 4267 ACACGT A^C[GA][CT]GT
5361 ACGTGT
AspI 2474 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3330 GACGAGGTC
BbeI 4158 GGCGCC GGCGC^C
4514 GGCGCC
BcgI 4421 CGATGAGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3629 GCAGCAGACTCG
BsaAI 2982 CACGTG [CT]AC^GT[GA]
4467 CACGTA
BsaI 4105 GAGACC GGTCTC(1/5)
2636 GGTCTC
BsaWI 3016 TCCGGA [AT]^CCGG[AT]
5015 TCCGGT
BsrBI 31 GAGCGG CCGCTC(-3/-3)
40 GAGCGG
BsrFI 121 GCCGGC [GA]^CCGG[CT]
4680 GCCGGC
BssHII 14 GCGCGC G^CGCGC
4769 GCGCGC
BssSI 3113 CACGAG CACGAG(-5/-1)
4778 CACGAG
BstXI 4336 CCAAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5159 CCAAAGCCCTGG
Cfr10I 121 GCCGGC [GA]^CCGG[CT]
4680 GCCGGC
KasI 4158 GGCGCC G^GCGCC
4514 GGCGCC
KspI 142 CCGCGG CCGC^GG
3985 CCGCGG
MseI 4752 TTAA T^TAA
5389 TTAA
NaeI 121 GCCGGC GCC^GGC
4680 GCCGGC
NarI 4158 GGCGCC GG^CGCC
4514 GGCGCC
NcoI 3757 CCATGG C^CATGG
3865 CCATGG
NgoMIV 121 GCCGGC G^CCGGC
4680 GCCGGC
PflFI 2474 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3330 GACGAGGTC
RsaI 4067 GTAC GT^AC
5019 GTAC
SacII 142 CCGCGG CCGC^GG
3985 CCGCGG
SapI 4115 GAAGAGC GCTCTTC(1/4)
4460 GAAGAGC
SfoI 4158 GGCGCC GGC^GCC
4514 GGCGCC
SphI 2959 GCATGC GCATG^C
4096 GCATGC
StuI 3784 AGGCCT AGG^CCT
4711 AGGCCT
Tru9I 4752 TTAA T^TAA
5389 TTAA
Tth111I 2474 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3330 GACGAGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccI AccII AciI AcyI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfiI SmaI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmaCI XmaI