##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BcgI BciVI BclI BfrI
BlnI BsaBI BseAI BsiWI BsmBI
BspEI BspHI BspLU11I BsrBI Bst1107I
BstBI BstZ17I CpoI EagI EclXI
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
MamI MfeI MluI MroI MunI
NaeI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AcsI 4046 GAATTT [GA]^AATT[CT]
AhdI 1726 GACCTCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 237 AGCGCT AGC^GCT
ApoI 4046 GAATTT [GA]^AATT[CT]
AspEI 1726 GACCTCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2548 GGATCC G^GATCC
BbeI 3659 GGCGCC GGCGC^C
BbsI 2822 GAAGAC GAAGAC(2/6)
BglII 84 AGATCT A^GATCT
BpuAI 2822 GAAGAC GAAGAC(2/6)
BsgI 674 GTGCAG GTGCAG(16/14)
BsiEI 3267 CGGTCG CG[GA][CT]^CG
Bsp1407I 1971 TGTACA T^GTACA
BspDI 3325 ATCGAT AT^CGAT
BspMI 2514 GCAGGT ACCTGC(4/8)
BsrGI 1971 TGTACA T^GTACA
BssHII 3182 GCGCGC G^CGCGC
BssSI 2386 CTCGTG CACGAG(-5/-1)
BstAPI 315 GCAGCGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 3325 ATCGAT AT^CGAT
Eam1105I 1726 GACCTCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2425 CTCTTC CTCTTC(1/4)
Eco47III 237 AGCGCT AGC^GCT
HincII 2592 GTTGAC GT[CT]^[GA]AC
HindII 2592 GTTGAC GT[CT]^[GA]AC
KasI 3659 GGCGCC G^GCGCC
Ksp632I 2425 CTCTTC CTCTTC(1/4)
NarI 3659 GGCGCC GG^CGCC
NspI 363 ACATGC [GA]CATG^[CT]
PflMI 2538 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 2424 GCTCTTC GCTCTTC(1/4)
SfoI 3659 GGCGCC GGC^GCC
SspBI 1971 TGTACA T^GTACA
Van91I 2538 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Alw44I 702 GTGCAC G^TGCAC
1259 GTGCAC
AocI 602 CCTGAGG CC^T[ACGT]AGG
1662 CCTGAGG
ApaLI 702 GTGCAC G^TGCAC
1259 GTGCAC
BbrPI 2304 CACGTG CAC^GTG
3780 CACGTG
BsaAI 2304 CACGTG [CT]AC^GT[GA]
3780 CACGTG
BsaWI 1273 TCCGGT [AT]^CCGG[AT]
3449 TCCGGT
BsmI 3059 GAATGC GAATGC(1/-1)
3539 GCATTC
BsrDI 1752 CATTGC GCAATG(2/0)
2178 CATTGC
BsrFI 1763 ACCGGC [GA]^CCGG[CT]
2027 GCCGGT
Bsu36I 602 CCTGAGG CC^T[ACGT]AGG
1662 CCTGAGG
Cfr10I 1763 ACCGGC [GA]^CCGG[CT]
2027 GCCGGT
DraI 3909 TTTAAA TTT^AAA
3948 TTTAAA
DraIII 160 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
996 CACTCTGTG
DrdI 1456 GACCCCTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2959 GACCCCGGGGTC
Eco81I 602 CCTGAGG CC^T[ACGT]AGG
1662 CCTGAGG
HgaI 470 GACGC GACGC(5/10)
261 GCGTC
NheI 221 GCTAGC G^CTAGC
3694 GCTAGC
PmaCI 2304 CACGTG CAC^GTG
3780 CACGTG
PmlI 2304 CACGTG CAC^GTG
3780 CACGTG
SacI 3226 GAGCTC GAGCT^C
4007 GAGCTC
SexAI 66 ACCAGGT A^CC[AT]GGT
3991 ACCAGGT
SmlI 641 CTTGAG C^T[CT][GA]AG
857 CTCAAG
Tsp509I 3980 AATT ^AATT
4047 AATT
XcmI 2534 CCACCCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3779 CCACGTGCGACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaHI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I TspRI TthHB8I XhoII XmaCI
XmaI