##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BfrI BglII BlnI
BsiWI BspDI BstBI ClaI CpoI
DraIII Eam1105I EcoRV FseI FspI
HindIII HpaI KpnI KspI MfeI
MluI MunI NdeI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacI SacII SbfI ScaI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccIII 342 TCCGGA T^CCGGA
Asp700I 1747 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1388 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 825 GGATCC G^GATCC
BbvCI 3173 GCTGAGG CCTCAGC(-5/-2)
BcgI 1953 GCATTCCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1833 TGATCA T^GATCA
BlpI 481 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 481 GCTGAGC GC^T[ACGT]AGC
BsaBI 130 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 342 TCCGGA [AT]^CCGG[AT]
BseAI 342 TCCGGA T^CCGGA
BspEI 342 TCCGGA T^CCGGA
BspHI 1830 TCATGA T^CATGA
BspLU11I 2016 ACATGT A^CATGT
BsrFI 2678 GCCGGC [GA]^CCGG[CT]
BssSI 2970 CTCGTG CACGAG(-5/-1)
Bst1107I 947 GTATAC GTA^TAC
BstAPI 1252 GCATTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 688 GGTGACC G^GT[ACGT]ACC
BstPI 688 GGTGACC G^GT[ACGT]ACC
BstZ17I 947 GTATAC GTA^TAC
CelII 481 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2678 GCCGGC [GA]^CCGG[CT]
EcoO65I 688 GGTGACC G^GT[ACGT]ACC
EcoT22I 1250 ATGCAT ATGCA^T
FbaI 1833 TGATCA T^GATCA
MamI 130 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 342 TCCGGA T^CCGGA
NaeI 2678 GCCGGC GCC^GGC
NgoMIV 2678 GCCGGC G^CCGGC
NsiI 1250 ATGCAT ATGCA^T
PflFI 1388 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2383 GACGTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1830 TCATGA T^CATGA
SalI 2930 GTCGAC G^TCGAC
SexAI 413 ACCTGGT A^CC[AT]GGT
Tth111I 1388 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3949 TCTAGA T^CTAGA
XmnI 1747 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AocI 1534 CCTGAGG CC^T[ACGT]AGG
2906 CCTCAGG
Aor51HI 1479 AGCGCT AGC^GCT
1857 AGCGCT
ApaI 1842 GGGCCC GGGCC^C
3669 GGGCCC
BalI 369 TGGCCA TGG^CCA
4121 TGGCCA
BbrPI 431 CACGTG CAC^GTG
962 CACGTG
BciVI 3251 GGATAC GTATCC(6/5)
3889 GTATCC
BsaAI 431 CACGTG [CT]AC^GT[GA]
962 CACGTG
BsmBI 333 GAGACG CGTCTC(1/5)
2270 GAGACG
BsmI 778 GAATGC GAATGC(1/-1)
1953 GCATTC
BsrBI 8 CCGCTC CCGCTC(-3/-3)
2161 GAGCGG
BsrDI 571 GCAATG GCAATG(2/0)
4107 GCAATG
BssHII 44 GCGCGC G^CGCGC
46 GCGCGC
BstXI 234 CCAAAATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1233 CCATGAAGCTGG
Bsu36I 1534 CCTGAGG CC^T[ACGT]AGG
2906 CCTCAGG
DrdI 500 GACACTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1493 GACCCGGACGTC
EagI 1086 CGGCCG C^GGCCG
2951 CGGCCG
EclXI 1086 CGGCCG C^GGCCG
2951 CGGCCG
Eco47III 1479 AGCGCT AGC^GCT
1857 AGCGCT
Eco52I 1086 CGGCCG C^GGCCG
2951 CGGCCG
Eco81I 1534 CCTGAGG CC^T[ACGT]AGG
2906 CCTCAGG
EcoRI 806 GAATTC G^AATTC
1712 GAATTC
HincII 767 GTTGAC GT[CT]^[GA]AC
2930 GTCGAC
HindII 767 GTTGAC GT[CT]^[GA]AC
2930 GTCGAC
MluNI 369 TGGCCA TGG^CCA
4121 TGGCCA
MscI 369 TGGCCA TGG^CCA
4121 TGGCCA
NcoI 3123 CCATGG C^CATGG
3673 CCATGG
PflMI 1048 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2079 CCAAGGACTGG
PmaCI 431 CACGTG CAC^GTG
962 CACGTG
PmlI 431 CACGTG CAC^GTG
962 CACGTG
SapI 2666 GAAGAGC GCTCTTC(1/4)
1105 GCTCTTC
Van91I 1048 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2079 CCAAGGACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AatII AccI AccII AciI AcsI
AcyI AfaI AflIII AluI Alw44I
AlwI AlwNI ApaLI ApoI AspHI
AvaI AvaII BanI BanII BbeI
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmaCI XmaI