##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Aor51HI AscI AseI AsnI Asp718I
AviII AvrII BamHI BbsI BcgI
BciVI BclI BglII BlnI BpuAI
BseAI BsiWI BspEI BsrBI BsrFI
BssHII BstBI Cfr10I CpoI Eco47III
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI MluI MroI
NaeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PshAI PshBI Psp1406I PvuI RsrII
SacI SalI SbfI SexAI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
StuI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AcyI 4191 GGCGCC G[GA]^CG[CT]C
AflII 2434 CTTAAG C^TTAAG
AhdI 1394 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 2314 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1394 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4317 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 4191 GGCGCC GGCGC^C
BfrI 2434 CTTAAG C^TTAAG
BlpI 517 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 517 GCTCAGC GC^T[ACGT]AGC
BsaBI 3081 GATTAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 4191 GGCGCC G[GA]^CG[CT]C
BsaWI 1106 ACCGGA [AT]^CCGG[AT]
BsiEI 4040 CGGCCG CG[GA][CT]^CG
BspDI 1321 ATCGAT AT^CGAT
BspHI 4101 TCATGA T^CATGA
BssSI 2730 CACGAG CACGAG(-5/-1)
Bst1107I 462 GTATAC GTA^TAC
BstYI 705 GGATCT [GA]^GATC[CT]
BstZ17I 462 GTATAC GTA^TAC
CelII 517 GCTCAGC GC^T[ACGT]AGC
ClaI 1321 ATCGAT AT^CGAT
DraIII 1457 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 4040 CGGCCG C^GGCCG
Eam1105I 1394 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 4040 CGGCCG C^GGCCG
Eco52I 4040 CGGCCG C^GGCCG
EcoRI 1434 GAATTC G^AATTC
HgaI 2491 GCGTC GACGC(5/10)
Hin1I 4191 GGCGCC G[GA]^CG[CT]C
HincII 2153 GTTGAC GT[CT]^[GA]AC
HindII 2153 GTTGAC GT[CT]^[GA]AC
KasI 4191 GGCGCC G^GCGCC
KspI 82 CCGCGG CCGC^GG
MamI 3081 GATTAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 705 GGATCT [GA]^GATC[CT]
NarI 4191 GGCGCC GG^CGCC
NcoI 3415 CCATGG C^CATGG
NdeI 1763 CATATG CA^TATG
PflFI 4317 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2184 CCACGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 3019 GTTTAAAC GTTT^AAAC
PpuMI 1127 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 4101 TCATGA T^CATGA
SacII 82 CCGCGG CCGC^GG
SapI 1448 GCTCTTC GCTCTTC(1/4)
ScaI 2198 AGTACT AGT^ACT
SfoI 4191 GGCGCC GGC^GCC
SmaI 755 CCCGGG CCC^GGG
Tth111I 4317 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2184 CCACGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1565 TCTAGA T^CTAGA
XcmI 1278 CCAGATTCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 705 GGATCT [GA]^GATC[CT]
XmaCI 755 CCCGGG C^CCGGG
XmaI 755 CCCGGG C^CCGGG
XmnI 2314 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 1434 GAATTC [GA]^AATT[CT]
2386 GAATTT
ApoI 1434 GAATTC [GA]^AATT[CT]
2386 GAATTT
BbvCI 1072 CCTCAGC CCTCAGC(-5/-2)
4219 GCTGAGG
BglI 3260 GCCCCCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3528 GCCCCCGAGGC
BsmBI 2492 CGTCTC CGTCTC(1/5)
4322 CGTCTC
BsmI 827 GAATGC GAATGC(1/-1)
329 GCATTC
BstEII 1603 GGTGACC G^GT[ACGT]ACC
1977 GGTCACC
BstPI 1603 GGTGACC G^GT[ACGT]ACC
1977 GGTCACC
BstXI 807 CCAAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4071 CCAGCACTTTGG
DraI 2578 TTTAAA TTT^AAA
3020 TTTAAA
DrdI 243 GACAAAACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3980 GACCAGATGGTC
EarI 1449 CTCTTC CTCTTC(1/4)
2554 GAAGAG
EcoNI 992 CCTATGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1572 CCTAAACAAGG
EcoO65I 1603 GGTGACC G^GT[ACGT]ACC
1977 GGTCACC
HaeII 397 GGCGCT [GA]GCGC^[CT]
4191 GGCGCC
Ksp632I 1449 CTCTTC CTCTTC(1/4)
2554 GAAGAG
PstI 3991 CTGCAG CTGCA^G
4282 CTGCAG
SfiI 3259 GGCCCCCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3527 GGCCCCCGAGGCC
SphI 2011 GCATGC GCATG^C
2128 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AccII AciI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI AspHI AvaI AvaII
BalI BanI BanII BbrPI BbvI
BcnI BfaI BmyI BpmI BsaAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspLU11I BspMI BsrDI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstUI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MfeI MluNI MnlI MscI
MseI MslI MspA1I MspI MunI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PmaCI
PmlI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I