##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AccIII AclI AhdI AscI AseI
AsnI AspEI AviII AvrII BbeI
BbrPI BciVI BlnI BsaAI BseAI
BsiWI BspDI BspEI BssHII BstBI
ClaI CpoI DraI Eam1105I EcoRI
EcoRV EcoT22I FseI FspI HindIII
HpaI KasI MroI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SalI ScaI SfiI
SfoI SfuI SnaBI SpeI SphI
SspI SwaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 4026 GGTACC G^GTACC
AcsI 3111 GAATTT [GA]^AATT[CT]
AflII 2404 CTTAAG C^TTAAG
Aor51HI 654 AGCGCT AGC^GCT
ApoI 3111 GAATTT [GA]^AATT[CT]
Asp718I 4026 GGTACC G^GTACC
AspI 492 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 182 GGATCC G^GATCC
BcgI 3511 CGATTCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1601 TGATCA T^GATCA
BfrI 2404 CTTAAG C^TTAAG
BglI 3288 GCCATGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4049 AGATCT A^GATCT
BlpI 2686 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2686 GCTCAGC GC^T[ACGT]AGC
BsmI 3137 GAATGC GAATGC(1/-1)
BspHI 1964 TCATGA T^CATGA
BspLU11I 3063 ACATGT A^CATGT
BsrBI 2427 CCGCTC CCGCTC(-3/-3)
BssSI 387 CACGAG CACGAG(-5/-1)
Bst1107I 4142 GTATAC GTA^TAC
BstAPI 3486 GCAACTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 915 GGTGACC G^GT[ACGT]ACC
BstPI 915 GGTGACC G^GT[ACGT]ACC
BstZ17I 4142 GTATAC GTA^TAC
CelII 2686 GCTCAGC GC^T[ACGT]AGC
DraIII 1374 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 654 AGCGCT AGC^GCT
EcoO65I 915 GGTGACC G^GT[ACGT]ACC
FbaI 1601 TGATCA T^GATCA
KpnI 4026 GGTACC GGTAC^C
KspI 1932 CCGCGG CCGC^GG
MfeI 3985 CAATTG C^AATTG
MluI 4 ACGCGT A^CGCGT
MunI 3985 CAATTG C^AATTG
PaeR7I 2669 CTCGAG C^TCGAG
PflFI 492 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1964 TCATGA T^CATGA
SacI 1246 GAGCTC GAGCT^C
SacII 1932 CCGCGG CCGC^GG
SexAI 4029 ACCTGGT A^CC[AT]GGT
SgrAI 467 CACCGGTG C[GA]^CCGG[CT]G
StuI 141 AGGCCT AGG^CCT
Tth111I 492 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 154 TCTAGA T^CTAGA
XhoI 2669 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AatII 943 GACGTC GACGT^C
2786 GACGTC
AflIII 4 ACGCGT A^C[GA][CT]GT
3063 ACATGT
AgeI 468 ACCGGT A^CCGGT
2480 ACCGGT
Alw44I 1035 GTGCAC G^TGCAC
1396 GTGCAC
ApaI 671 GGGCCC GGGCC^C
1390 GGGCCC
ApaLI 1035 GTGCAC G^TGCAC
1396 GTGCAC
Asp700I 2394 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2418 GAAAGCCTTC
BbvCI 2743 GCTGAGG CCTCAGC(-5/-2)
2848 GCTGAGG
BfaI 155 CTAG C^TAG
4062 CTAG
BsaWI 468 ACCGGT [AT]^CCGG[AT]
2480 ACCGGT
BsrDI 2301 CATTGC GCAATG(2/0)
4087 GCAATG
BsrFI 468 ACCGGT [GA]^CCGG[CT]
2480 ACCGGT
Cfr10I 468 ACCGGT [GA]^CCGG[CT]
2480 ACCGGT
HaeII 654 AGCGCT [GA]GCGC^[CT]
1099 AGCGCC
MaeI 155 CTAG C^TAG
4062 CTAG
PflMI 575 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1941 CCAGCAGATGG
PinAI 468 ACCGGT A^CCGGT
2480 ACCGGT
SbfI 1530 CCTGCAGG CCTGCA^GG
2123 CCTGCAGG
Sse8387I 1530 CCTGCAGG CCTGCA^GG
2123 CCTGCAGG
Van91I 575 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1941 CCAGCAGATGG
XmnI 2394 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2418 GAAAGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaBI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EagI EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmaCI XmaI