##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AlwNI Aor51HI ApaI
AscI AseI AsnI Asp718I AvaI
BbrPI BbvCI BciVI BfrI BlpI
Bpu1102I BsaAI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BsrBI BssHII Bst1107I BstBI BstZ17I
CelII ClaI CpoI DraIII DsaI
EagI EclXI Eco47III Eco52I EcoRI
EcoT22I FauI FseI HgaI KpnI
KspI MluI MroI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SfuI SgrAI
SmaI SnaBI Sse8387I SwaI Van91I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AcyI 2217 GGCGCC G[GA]^CG[CT]C
AflIII 2976 ACATGT A^C[GA][CT]GT
AhdI 3390 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 479 GTGCAC G^TGCAC
AocI 3551 CCTGAGG CC^T[ACGT]AGG
ApaLI 479 GTGCAC G^TGCAC
Asp700I 254 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3390 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2183 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 735 TGCGCA TGC^GCA
AvrII 1957 CCTAGG C^CTAGG
BamHI 1455 GGATCC G^GATCC
BbeI 2217 GGCGCC GGCGC^C
BcgI 2899 GCATTATCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 2125 GCCCTCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1957 CCTAGG C^CTAGG
BsaHI 2217 GGCGCC G[GA]^CG[CT]C
Bsp1407I 369 TGTACA T^GTACA
BspHI 1914 TCATGA T^CATGA
BspLU11I 2976 ACATGT A^CATGT
BsrDI 3423 CATTGC GCAATG(2/0)
BsrFI 980 GCCGGT [GA]^CCGG[CT]
BsrGI 369 TGTACA T^GTACA
BssSI 2435 CTCGTG CACGAG(-5/-1)
Bsu36I 3551 CCTGAGG CC^T[ACGT]AGG
Cfr10I 980 GCCGGT [GA]^CCGG[CT]
DraI 56 TTTAAA TTT^AAA
DrdI 2195 GACACGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3390 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3551 CCTGAGG CC^T[ACGT]AGG
EcoRV 224 GATATC GAT^ATC
FspI 735 TGCGCA TGC^GCA
HaeII 2217 GGCGCC [GA]GCGC^[CT]
Hin1I 2217 GGCGCC G[GA]^CG[CT]C
HindIII 1628 AAGCTT A^AGCTT
HpaI 3110 GTTAAC GTT^AAC
KasI 2217 GGCGCC G^GCGCC
MfeI 2718 CAATTG C^AATTG
MunI 2718 CAATTG C^AATTG
NarI 2217 GGCGCC GG^CGCC
PacI 3726 TTAATTAA TTAAT^TAA
PflFI 2183 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 112 GACCCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1914 TCATGA T^CATGA
SacI 1004 GAGCTC GAGCT^C
ScaI 2415 AGTACT AGT^ACT
SfoI 2217 GGCGCC GGC^GCC
SpeI 31 ACTAGT A^CTAGT
SspBI 369 TGTACA T^GTACA
StuI 2132 AGGCCT AGG^CCT
Tth111I 2183 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 254 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 652 CGCG CG^CG
707 CGCG
AspHI 479 GTGCAC G[AT]GC[AT]^C
1004 GAGCTC
BalI 78 TGGCCA TGG^CCA
309 TGGCCA
BbsI 3065 GAAGAC GAAGAC(2/6)
3398 GTCTTC
BclI 1203 TGATCA T^GATCA
1324 TGATCA
BpuAI 3065 GAAGAC GAAGAC(2/6)
3398 GTCTTC
BsaI 2708 GAGACC GGTCTC(1/5)
578 GGTCTC
BsiHKAI 479 GTGCAC G[AT]GC[AT]^C
1004 GAGCTC
BsmFI 494 GGGAC GGGAC(10/14)
3326 GGGAC
BsmI 154 GAATGC GAATGC(1/-1)
3341 GAATGC
BspMI 702 ACCTGC ACCTGC(4/8)
2243 GCAGGT
BstEII 433 GGTCACC G^GT[ACGT]ACC
1033 GGTGACC
BstPI 433 GGTCACC G^GT[ACGT]ACC
1033 GGTGACC
BstUI 652 CGCG CG^CG
707 CGCG
BstXI 1038 CCAAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1385 CCAGGAAAATGG
DraII 3569 AGGGCCT [GA]G^G[ACGT]CC[CT]
3650 AGGCCCC
EcoO109I 3569 AGGGCCT [GA]G^G[ACGT]CC[CT]
3650 AGGCCCC
EcoO65I 433 GGTCACC G^GT[ACGT]ACC
1033 GGTGACC
EcoT14I 1957 CCTAGG C^C[AT][AT]GG
3322 CCTTGG
FbaI 1203 TGATCA T^GATCA
1324 TGATCA
MaeII 589 ACGT A^CGT
630 ACGT
MluNI 78 TGGCCA TGG^CCA
309 TGGCCA
MscI 78 TGGCCA TGG^CCA
309 TGGCCA
MslI 2231 CACATTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2431 CATTCTCGTG
MvnI 652 CGCG CG^CG
707 CGCG
SphI 711 GCATGC GCATG^C
732 GCATGC
SspI 1239 AATATT AAT^ATT
2949 AATATT
StyI 1957 CCTAGG C^C[AT][AT]GG
3322 CCTTGG
XcmI 2555 CCAGAATTAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2922 CCATTACTGAATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanI BanII BbvI
BcnI BfaI BglII BmyI BpmI
BsaBI BsaJI BseRI BsgI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EarI EcoNI EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MamI MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII