##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AspEI
AviII AvrII BbrPI BfrI BlnI
BsiEI BsiWI BsmI BspDI BspLU11I
BssHII BstBI ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FseI FspI
HpaI KspI MfeI MluI MunI
NaeI NgoMIV NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
StuI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 2182 GGTACC G^GTACC
AcsI 646 AAATTC [GA]^AATT[CT]
ApoI 646 AAATTC [GA]^AATT[CT]
AseI 3903 ATTAAT AT^TAAT
AsnI 3903 ATTAAT AT^TAAT
Asp700I 188 GAATCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2182 GGTACC G^GTACC
BamHI 1306 GGATCC G^GATCC
BciVI 3957 GTATCC GTATCC(6/5)
BfaI 993 CTAG C^TAG
BlpI 260 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 260 GCTCAGC GC^T[ACGT]AGC
BsaAI 3936 TACGTG [CT]AC^GT[GA]
BsgI 3069 GTGCAG GTGCAG(16/14)
BsrBI 1835 CCGCTC CCGCTC(-3/-3)
Bst1107I 2508 GTATAC GTA^TAC
BstAPI 1637 GCAAGGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3030 GGTCACC G^GT[ACGT]ACC
BstPI 3030 GGTCACC G^GT[ACGT]ACC
BstZ17I 2508 GTATAC GTA^TAC
CelII 260 GCTCAGC GC^T[ACGT]AGC
DraIII 3033 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 2587 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3030 GGTCACC G^GT[ACGT]ACC
HindIII 3599 AAGCTT A^AGCTT
KpnI 2182 GGTACC GGTAC^C
MaeI 993 CTAG C^TAG
NdeI 3385 CATATG CA^TATG
PaeR7I 708 CTCGAG C^TCGAG
PshAI 3985 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3903 ATTAAT AT^TAAT
SexAI 2866 ACCTGGT A^CC[AT]GGT
SspI 3643 AATATT AAT^ATT
XhoI 708 CTCGAG C^TCGAG
XmnI 188 GAATCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 2349 ACGTGT A^C[GA][CT]GT
3937 ACGTGT
AocI 594 CCTGAGG CC^T[ACGT]AGG
1914 CCTGAGG
ApaI 229 GGGCCC GGGCC^C
1205 GGGCCC
BbvCI 175 CCTCAGC CCTCAGC(-5/-2)
456 GCTGAGG
BcgI 66 GCACGGACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
105 GCAGCCATGTCG
BglI 108 GCCATGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2403 GCCAACCAGGC
BglII 2516 AGATCT A^GATCT
3920 AGATCT
BsmBI 2819 CGTCTC CGTCTC(1/5)
1672 GAGACG
Bsp1407I 970 TGTACA T^GTACA
3476 TGTACA
BspHI 1087 TCATGA T^CATGA
2629 TCATGA
BsrDI 866 CATTGC GCAATG(2/0)
4018 CATTGC
BsrFI 5 ACCGGC [GA]^CCGG[CT]
1372 ACCGGC
BsrGI 970 TGTACA T^GTACA
3476 TGTACA
BstXI 1316 CCATGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2194 CCACATTCATGG
Bsu36I 594 CCTGAGG CC^T[ACGT]AGG
1914 CCTGAGG
Cfr10I 5 ACCGGC [GA]^CCGG[CT]
1372 ACCGGC
Eco81I 594 CCTGAGG CC^T[ACGT]AGG
1914 CCTGAGG
HincII 330 GTCAAC GT[CT]^[GA]AC
1002 GTCAAC
HindII 330 GTCAAC GT[CT]^[GA]AC
1002 GTCAAC
PflMI 266 CCAGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1606 CCATTGTGTGG
PpuMI 2722 GGGTCCT [GA]G^G[AT]CC[CT]
3231 GGGTCCT
RcaI 1087 TCATGA T^CATGA
2629 TCATGA
SacI 2115 GAGCTC GAGCT^C
2661 GAGCTC
SmaI 1627 CCCGGG CCC^GGG
2186 CCCGGG
SspBI 970 TGTACA T^GTACA
3476 TGTACA
Van91I 266 CCAGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1606 CCATTGTGTGG
XcmI 1348 CCATTGTGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4219 CCACAAAGATTCTGG
XmaCI 1627 CCCGGG C^CCGGG
2186 CCCGGG
XmaI 1627 CCCGGG C^CCGGG
2186 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AccII AccIII AciI AcyI
AfaI AluI AlwI AlwNI AspHI
AspI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvI BclI
BcnI BmyI BpmI BpuAI BsaBI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspEI BspMI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeII MaeIII MamI
MboI MboII MflI MluNI MnlI
MroI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PflFI PleI PstI PvuII
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SfoI SmlI SphI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XhoII