################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## AatII Acc65I AccI AclI AflIII AgeI ApaI AseI AsnI Asp718I AspI AviII BbrPI BciVI BclI BsiWI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DrdI EcoO65I EcoRV EcoT22I FbaI FspI HincII HindII HindIII HpaI KpnI KspI MfeI MluI MunI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SfiI SfuI SgrAI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AflII 3392 CTTAAG C^TTAAG AhdI 3000 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 3147 GTGCAC G^TGCAC AocI 747 CCTGAGG CC^T[ACGT]AGG Aor51HI 3658 AGCGCT AGC^GCT ApaLI 3147 GTGCAC G^TGCAC AscI 28 GGCGCGCC GG^CGCGCC AspEI 3000 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 3915 CCTAGG C^CTAGG BcgI 1503 GCAGAGCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 3392 CTTAAG C^TTAAG BglI 2949 GCCCTCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1894 AGATCT A^GATCT BlnI 3915 CCTAGG C^CTAGG BsaBI 1409 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 2097 GTGCAG GTGCAG(16/14) BsiEI 2456 CGGCCG CG[GA][CT]^CG BsmI 3417 GAATGC GAATGC(1/-1) BstXI 2227 CCACCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 747 CCTGAGG CC^T[ACGT]AGG EagI 2456 CGGCCG C^GGCCG Eam1105I 3000 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 2456 CGGCCG C^GGCCG Eco47III 3658 AGCGCT AGC^GCT Eco52I 2456 CGGCCG C^GGCCG Eco81I 747 CCTGAGG CC^T[ACGT]AGG FseI 2453 GGCCGGCC GGCCGG^CC MamI 1409 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NdeI 2516 CATATG CA^TATG NspI 142 ACATGC [GA]CATG^[CT] SacI 808 GAGCTC GAGCT^C ScaI 3363 AGTACT AGT^ACT SexAI 2413 ACCTGGT A^CC[AT]GGT SnaBI 3172 TACGTA TAC^GTA StuI 1424 AGGCCT AGG^CCT XcmI 3809 CCAGTGCTCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccIII 1057 TCCGGA T^CCGGA 4051 TCCGGA Asp700I 1892 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3024 GAACACCTTC BalI 754 TGGCCA TGG^CCA 2956 TGGCCA BamHI 923 GGATCC G^GATCC 2637 GGATCC BanI 2170 GGCGCC G^G[CT][GA]CC 2427 GGCGCC BbeI 2170 GGCGCC GGCGC^C 2427 GGCGCC BseAI 1057 TCCGGA T^CCGGA 4051 TCCGGA BspEI 1057 TCCGGA T^CCGGA 4051 TCCGGA BspMI 1830 ACCTGC ACCTGC(4/8) 2099 GCAGGT BsrBI 1332 CCGCTC CCGCTC(-3/-3) 1478 GAGCGG BssHII 29 GCGCGC G^CGCGC 67 GCGCGC DraIII 2925 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG 3457 CACATAGTG KasI 2170 GGCGCC G^GCGCC 2427 GGCGCC MluNI 754 TGGCCA TGG^CCA 2956 TGGCCA MroI 1057 TCCGGA T^CCGGA 4051 TCCGGA MscI 754 TGGCCA TGG^CCA 2956 TGGCCA MslI 2922 CATCACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2954 CATGGCCATG NarI 2170 GGCGCC GG^CGCC 2427 GGCGCC PflMI 1868 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3642 CCACTGGCTGG SfoI 2170 GGCGCC GGC^GCC 2427 GGCGCC Van91I 1868 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3642 CCACTGGCTGG XmnI 1892 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3024 GAACACCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanII BbsI BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmaCI XmaI