##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccI AclI AflIII
AgeI ApaI AseI AsnI Asp718I
AspI AviII BbrPI BciVI BclI
BsiWI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DrdI EcoO65I EcoRV EcoT22I
FbaI FspI HincII HindII HindIII
HpaI KpnI KspI MfeI MluI
MunI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SfiI SfuI SgrAI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AflII 3392 CTTAAG C^TTAAG
AhdI 3000 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3147 GTGCAC G^TGCAC
AocI 747 CCTGAGG CC^T[ACGT]AGG
Aor51HI 3658 AGCGCT AGC^GCT
ApaLI 3147 GTGCAC G^TGCAC
AscI 28 GGCGCGCC GG^CGCGCC
AspEI 3000 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3915 CCTAGG C^CTAGG
BcgI 1503 GCAGAGCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3392 CTTAAG C^TTAAG
BglI 2949 GCCCTCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1894 AGATCT A^GATCT
BlnI 3915 CCTAGG C^CTAGG
BsaBI 1409 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 2097 GTGCAG GTGCAG(16/14)
BsiEI 2456 CGGCCG CG[GA][CT]^CG
BsmI 3417 GAATGC GAATGC(1/-1)
BstXI 2227 CCACCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 747 CCTGAGG CC^T[ACGT]AGG
EagI 2456 CGGCCG C^GGCCG
Eam1105I 3000 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2456 CGGCCG C^GGCCG
Eco47III 3658 AGCGCT AGC^GCT
Eco52I 2456 CGGCCG C^GGCCG
Eco81I 747 CCTGAGG CC^T[ACGT]AGG
FseI 2453 GGCCGGCC GGCCGG^CC
MamI 1409 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 2516 CATATG CA^TATG
NspI 142 ACATGC [GA]CATG^[CT]
SacI 808 GAGCTC GAGCT^C
ScaI 3363 AGTACT AGT^ACT
SexAI 2413 ACCTGGT A^CC[AT]GGT
SnaBI 3172 TACGTA TAC^GTA
StuI 1424 AGGCCT AGG^CCT
XcmI 3809 CCAGTGCTCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccIII 1057 TCCGGA T^CCGGA
4051 TCCGGA
Asp700I 1892 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3024 GAACACCTTC
BalI 754 TGGCCA TGG^CCA
2956 TGGCCA
BamHI 923 GGATCC G^GATCC
2637 GGATCC
BanI 2170 GGCGCC G^G[CT][GA]CC
2427 GGCGCC
BbeI 2170 GGCGCC GGCGC^C
2427 GGCGCC
BseAI 1057 TCCGGA T^CCGGA
4051 TCCGGA
BspEI 1057 TCCGGA T^CCGGA
4051 TCCGGA
BspMI 1830 ACCTGC ACCTGC(4/8)
2099 GCAGGT
BsrBI 1332 CCGCTC CCGCTC(-3/-3)
1478 GAGCGG
BssHII 29 GCGCGC G^CGCGC
67 GCGCGC
DraIII 2925 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
3457 CACATAGTG
KasI 2170 GGCGCC G^GCGCC
2427 GGCGCC
MluNI 754 TGGCCA TGG^CCA
2956 TGGCCA
MroI 1057 TCCGGA T^CCGGA
4051 TCCGGA
MscI 754 TGGCCA TGG^CCA
2956 TGGCCA
MslI 2922 CATCACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2954 CATGGCCATG
NarI 2170 GGCGCC GG^CGCC
2427 GGCGCC
PflMI 1868 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3642 CCACTGGCTGG
SfoI 2170 GGCGCC GGC^GCC
2427 GGCGCC
Van91I 1868 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3642 CCACTGGCTGG
XmnI 1892 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3024 GAACACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanII BbsI BbvCI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmaCI
XmaI