##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII Acc65I AflII AgeI Aor51HI
AscI Asp700I Asp718I AviII AvrII
BbrPI BfrI BlnI BsaBI BsiWI
BsmBI BspDI BspLU11I BsrBI Bst1107I
BstZ17I ClaI CpoI Eco47III EcoRI
EcoRV FseI FspI HindIII HpaI
KpnI KspI MamI NdeI NheI
NotI PacI PaeR7I PinAI PmaCI
PmeI PmlI PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SgrAI SnaBI SpeI Sse8387I StuI
SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccIII 3093 TCCGGA T^CCGGA
AclI 984 AACGTT AA^CGTT
AflIII 385 ACGCGT A^C[GA][CT]GT
AhdI 3266 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1952 GGGCCC GGGCC^C
AseI 4080 ATTAAT AT^TAAT
AsnI 4080 ATTAAT AT^TAAT
AspEI 3266 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3135 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 298 GGATCC G^GATCC
BbsI 2823 GAAGAC GAAGAC(2/6)
BglII 2005 AGATCT A^GATCT
BpuAI 2823 GAAGAC GAAGAC(2/6)
BsaAI 3078 TACGTG [CT]AC^GT[GA]
BsaWI 3093 TCCGGA [AT]^CCGG[AT]
BseAI 3093 TCCGGA T^CCGGA
Bsp1407I 1853 TGTACA T^GTACA
BspEI 3093 TCCGGA T^CCGGA
BspMI 3147 ACCTGC ACCTGC(4/8)
BsrDI 3310 CATTGC GCAATG(2/0)
BsrGI 1853 TGTACA T^GTACA
BssHII 3102 GCGCGC G^CGCGC
BssSI 1222 CTCGTG CACGAG(-5/-1)
BstBI 3244 TTCGAA TT^CGAA
BstEII 685 GGTAACC G^GT[ACGT]ACC
BstPI 685 GGTAACC G^GT[ACGT]ACC
BstXI 3636 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 4069 TTTAAA TTT^AAA
DrdI 2468 GACCTTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 26 CGGCCG C^GGCCG
Eam1105I 3266 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 26 CGGCCG C^GGCCG
Eco52I 26 CGGCCG C^GGCCG
EcoNI 2941 CCTTCCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 685 GGTAACC G^GT[ACGT]ACC
EcoT22I 2312 ATGCAT ATGCA^T
MfeI 1231 CAATTG C^AATTG
MluI 385 ACGCGT A^CGCGT
MroI 3093 TCCGGA T^CCGGA
MunI 1231 CAATTG C^AATTG
NcoI 377 CCATGG C^CATGG
NruI 9 TCGCGA TCG^CGA
NsiI 2312 ATGCAT ATGCA^T
NspV 3244 TTCGAA TT^CGAA
PflFI 3135 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1276 GACTTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4080 ATTAAT AT^TAAT
Psp1406I 984 AACGTT AA^CGTT
SacI 1605 GAGCTC GAGCT^C
SfuI 3244 TTCGAA TT^CGAA
SspBI 1853 TGTACA T^GTACA
SspI 600 AATATT AAT^ATT
Tth111I 3135 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 3229 CCATTGATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 266 GTAGAC GT^[AC][GT]AC
1875 GTCTAC
Alw44I 3111 GTGCAC G^TGCAC
3201 GTGCAC
ApaLI 3111 GTGCAC G^TGCAC
3201 GTGCAC
BbvCI 1366 CCTCAGC CCTCAGC(-5/-2)
2887 CCTCAGC
BcgI 404 CGAGAGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
529 GCAGTAGTGTCG
BclI 2551 TGATCA T^GATCA
2881 TGATCA
BlpI 1602 GCTGAGC GC^T[ACGT]AGC
3964 GCTCAGC
Bpu1102I 1602 GCTGAGC GC^T[ACGT]AGC
3964 GCTCAGC
BsiEI 26 CGGCCG CG[GA][CT]^CG
443 CGGTCG
BsmI 1568 GAATGC GAATGC(1/-1)
3289 GCATTC
CelII 1602 GCTGAGC GC^T[ACGT]AGC
3964 GCTCAGC
DraIII 1651 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
2919 CACGGCGTG
FbaI 2551 TGATCA T^GATCA
2881 TGATCA
HincII 2577 GTCAAC GT[CT]^[GA]AC
3351 GTCAAC
HindII 2577 GTCAAC GT[CT]^[GA]AC
3351 GTCAAC
MslI 2141 CATGAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4042 CATTGTTATG
NaeI 24 GCCGGC GCC^GGC
397 GCCGGC
NgoMIV 24 GCCGGC G^CCGGC
397 GCCGGC
SapI 653 GAAGAGC GCTCTTC(1/4)
2618 GCTCTTC
SmaI 367 CCCGGG CCC^GGG
3810 CCCGGG
SmlI 1645 CTCAAG C^T[CT][GA]AG
3669 CTTGAG
XmaCI 367 CCCGGG C^CCGGG
3810 CCCGGG
XmaI 367 CCCGGG C^CCGGG
3810 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbvI BciVI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspHI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SphI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII