##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AviII AvrII BbeI BbvCI
BcgI BfrI BlnI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BspLU11I
BsrBI BsrFI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Cfr10I
ClaI CpoI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I FauI FseI
FspI KasI KpnI MluI MroI
NaeI NarI NgoMIV NotI NruI
NspV PacI PinAI PmeI PshAI
Psp1406I PvuI RsrII SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I StuI SwaI
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 66 enzymes) #
###################################
AatII 1872 GACGTC GACGT^C
AcyI 1872 GACGTC G[GA]^CG[CT]C
AflIII 2597 ACACGT A^C[GA][CT]GT
AhdI 3028 GACTATTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 3511 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1858 CCTAAGG CC^T[ACGT]AGG
AseI 659 ATTAAT AT^TAAT
AsnI 659 ATTAAT AT^TAAT
AspEI 3028 GACTATTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2913 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 81 CTCGAG C^[CT]CG[GA]G
BamHI 637 GGATCC G^GATCC
BanI 836 GGTGCC G^G[CT][GA]CC
BanII 78 GAGCTC G[GA]GC[CT]^C
BbrPI 2598 CACGTG CAC^GTG
BciVI 1196 GGATAC GTATCC(6/5)
BcnI 154 CCCGG CC^[GC]GG
BglI 1679 GCCTTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3332 AGATCT A^GATCT
BlpI 311 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 311 GCTCAGC GC^T[ACGT]AGC
BsaHI 1872 GACGTC G[GA]^CG[CT]C
BsgI 3122 GTGCAG GTGCAG(16/14)
BsoBI 81 CTCGAG C^[CT]CG[GA]G
Bsp1407I 3183 TGTACA T^GTACA
BspHI 2484 TCATGA T^CATGA
BspMI 1542 ACCTGC ACCTGC(4/8)
BsrGI 3183 TGTACA T^GTACA
Bsu36I 1858 CCTAAGG CC^T[ACGT]AGG
CelII 311 GCTCAGC GC^T[ACGT]AGC
DraIII 2181 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3028 GACTATTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1858 CCTAAGG CC^T[ACGT]AGG
EcoRI 778 GAATTC G^AATTC
EcoRV 2990 GATATC GAT^ATC
EcoT22I 3748 ATGCAT ATGCA^T
HaeII 29 GGCGCT [GA]GCGC^[CT]
HapII 155 CCGG C^CGG
Hin1I 1872 GACGTC G[GA]^CG[CT]C
HindIII 1020 AAGCTT A^AGCTT
HpaI 3116 GTTAAC GTT^AAC
HpaII 155 CCGG C^CGG
KspI 2506 CCGCGG CCGC^GG
MspI 155 CCGG C^CGG
NciI 154 CCCGG CC^[GC]GG
NheI 1582 GCTAGC G^CTAGC
NsiI 3748 ATGCAT ATGCA^T
PaeR7I 81 CTCGAG C^TCGAG
PflFI 2913 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2885 CCACAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2598 CACGTG CAC^GTG
PmlI 2598 CACGTG CAC^GTG
PshBI 659 ATTAAT AT^TAAT
PstI 1773 CTGCAG CTGCA^G
RcaI 2484 TCATGA T^CATGA
SacI 78 GAGCTC GAGCT^C
SacII 2506 CCGCGG CCGC^GG
SalI 2910 GTCGAC G^TCGAC
ScaI 797 AGTACT AGT^ACT
SpeI 2792 ACTAGT A^CTAGT
SspBI 3183 TGTACA T^GTACA
SspI 3220 AATATT AAT^ATT
Tth111I 2913 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2885 CCACAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2073 CCAGTGGGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 81 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
BalI 2070 TGGCCA TGG^CCA
2439 TGGCCA
BbsI 278 GAAGAC GAAGAC(2/6)
2471 GAAGAC
BpuAI 278 GAAGAC GAAGAC(2/6)
2471 GAAGAC
BsaAI 454 CACGTA [CT]AC^GT[GA]
2598 CACGTG
BsaI 1172 GAGACC GGTCTC(1/5)
1637 GAGACC
BsmFI 3292 GGGAC GGGAC(10/14)
2254 GTCCC
BsrDI 2443 CATTGC GCAATG(2/0)
2126 GCAATG
BssSI 3282 CTCGTG CACGAG(-5/-1)
3336 CTCGTG
BstAPI 1775 GCAGCTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3510 GCAGGGGCTGC
CfoI 30 GCGC GCG^C
862 GCGC
DraI 1552 TTTAAA TTT^AAA
3689 TTTAAA
DrdI 1519 GACTTTTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2377 GACTACACGGTC
EaeI 2070 TGGCCA [CT]^GGCC[GA]
2439 TGGCCA
HhaI 30 GCGC GCG^C
862 GCGC
HinP1I 30 GCGC G^CGC
862 GCGC
MfeI 2024 CAATTG C^AATTG
2433 CAATTG
MluNI 2070 TGGCCA TGG^CCA
2439 TGGCCA
MscI 2070 TGGCCA TGG^CCA
2439 TGGCCA
MunI 2024 CAATTG C^AATTG
2433 CAATTG
NcoI 3321 CCATGG C^CATGG
3388 CCATGG
NdeI 3257 CATATG CA^TATG
3501 CATATG
PvuII 227 CAGCTG CAG^CTG
2496 CAGCTG
SmlI 81 CTCGAG C^T[CT][GA]AG
773 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI ApoI Asp700I AspHI
AvaII BbvI BclI BfaI BmyI
BpmI BsaBI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HgaI HincII HindII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MvnI MwoI NdeII NlaIII NlaIV
NspI PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SphI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI