##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII AccI AccIII AcyI AflII
AgeI Aor51HI AscI AspI AvaII
AvrII BalI BbeI BbrPI BbvCI
BcgI BciVI BfrI BlnI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BspHI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Cfr10I ClaI CpoI DrdI EaeI
EagI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV FseI HaeII Hin1I
HpaI KasI MamI MfeI MluI
MluNI MroI MscI MunI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PvuI PvuII RcaI RsrII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I StuI SwaI Tth111I
Van91I XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 583 GGTACC G^GTACC
AhdI 14 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 583 GGTACC G^GTACC
AspEI 14 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 406 CCCGGG C^[CT]CG[GA]G
AviII 56 TGCGCA TGC^GCA
BamHI 619 GGATCC G^GATCC
BanI 583 GGTACC G^G[CT][GA]CC
BclI 605 TGATCA T^GATCA
BfaI 3517 CTAG C^TAG
BglI 2968 GCCCTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1364 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1364 GCTCAGC GC^T[ACGT]AGC
BsaAI 3571 CACGTA [CT]AC^GT[GA]
BsgI 3577 GTGCAG GTGCAG(16/14)
BsmBI 485 CGTCTC CGTCTC(1/5)
BsoBI 406 CCCGGG C^[CT]CG[GA]G
BspLU11I 358 ACATGT A^CATGT
BspMI 224 GCAGGT ACCTGC(4/8)
BstXI 100 CCAGGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1364 GCTCAGC GC^T[ACGT]AGC
DraI 597 TTTAAA TTT^AAA
DraIII 3571 CACGTAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 14 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 605 TGATCA T^GATCA
FspI 56 TGCGCA TGC^GCA
HincII 289 GTTGAC GT[CT]^[GA]AC
HindII 289 GTTGAC GT[CT]^[GA]AC
KpnI 583 GGTACC GGTAC^C
KspI 247 CCGCGG CCGC^GG
MaeI 3517 CTAG C^TAG
NdeI 3134 CATATG CA^TATG
PstI 870 CTGCAG CTGCA^G
SacI 1362 GAGCTC GAGCT^C
SacII 247 CCGCGG CCGC^GG
SapI 543 GAAGAGC GCTCTTC(1/4)
SmaI 406 CCCGGG CCC^GGG
SmlI 2299 CTCAAG C^T[CT][GA]AG
TaqI 436 TCGA T^CGA
TthHB8I 436 TCGA T^CGA
XmaCI 406 CCCGGG C^CCGGG
XmaI 406 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AclI 2949 AACGTT AA^CGTT
3285 AACGTT
AflIII 358 ACATGT A^C[GA][CT]GT
3570 ACACGT
AlwNI 420 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
2178 CAGTGACTG
AocI 2970 CCTCAGG CC^T[ACGT]AGG
3542 CCTGAGG
AseI 806 ATTAAT AT^TAAT
1264 ATTAAT
AsnI 806 ATTAAT AT^TAAT
1264 ATTAAT
BglII 1789 AGATCT A^GATCT
2359 AGATCT
Bsu36I 2970 CCTCAGG CC^T[ACGT]AGG
3542 CCTGAGG
Eco81I 2970 CCTCAGG CC^T[ACGT]AGG
3542 CCTGAGG
EcoT22I 3496 ATGCAT ATGCA^T
3551 ATGCAT
HgaI 443 GACGC GACGC(5/10)
74 GCGTC
MspA1I 247 CCGCGG C[AC]G^C[GT]G
1202 CCGCTG
NcoI 549 CCATGG C^CATGG
3693 CCATGG
NsiI 3496 ATGCAT ATGCA^T
3551 ATGCAT
NspI 358 ACATGT [GA]CATG^[CT]
3520 GCATGT
PshBI 806 ATTAAT AT^TAAT
1264 ATTAAT
Psp1406I 2949 AACGTT AA^CGTT
3285 AACGTT
SfcI 473 CTACAG C^T[GA][CT]AG
870 CTGCAG
SspI 656 AATATT AAT^ATT
826 AATATT
TfiI 990 GAATC G^A[AT]TC
1673 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
Alw44I AlwI ApaI ApaLI ApoI
Asp700I AspHI BanII BbsI BbvI
BcnI BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SspBI StyI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII XmnI