##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
Acc65I AccIII AgeI AhdI Aor51HI
AscI AseI AsnI Asp718I AspEI
AviII BalI BamHI BbrPI BcgI
BclI BglI BglII BsaBI BseAI
BsgI BsiWI BspDI BspEI BspHI
BsrBI BssHII BssSI BstBI ClaI
CpoI DrdI Eam1105I Eco47III EcoNI
EcoRI FbaI FseI FspI KpnI
MamI MfeI MluI MluNI MroI
MscI MunI NcoI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI PvuI PvuII RcaI RsrII
SacI SalI SapI SbfI SfiI
SfuI SgrAI SnaBI Sse8387I SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 338 GACGTC GACGT^C
AclI 3416 AACGTT AA^CGTT
Alw44I 1200 GTGCAC G^TGCAC
AocI 2292 CCTGAGG CC^T[ACGT]AGG
ApaI 58 GGGCCC GGGCC^C
ApaLI 1200 GTGCAC G^TGCAC
AspHI 1200 GTGCAC G[AT]GC[AT]^C
AspI 1169 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3338 CCTAGG C^CTAGG
BbeI 1291 GGCGCC GGCGC^C
BciVI 461 GGATAC GTATCC(6/5)
BlnI 3338 CCTAGG C^CTAGG
BsaWI 34 TCCGGT [AT]^CCGG[AT]
BseRI 105 GAGGAG GAGGAG(10/8)
BsiHKAI 1200 GTGCAC G[AT]GC[AT]^C
Bsp1407I 854 TGTACA T^GTACA
BspMI 468 GCAGGT ACCTGC(4/8)
BsrGI 854 TGTACA T^GTACA
BstAPI 1709 GCAGGGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2124 GGTTACC G^GT[ACGT]ACC
BstPI 2124 GGTTACC G^GT[ACGT]ACC
Bsu36I 2292 CCTGAGG CC^T[ACGT]AGG
DraIII 3882 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2292 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2124 GGTTACC G^GT[ACGT]ACC
EcoRV 1688 GATATC GAT^ATC
HgaI 1303 GACGC GACGC(5/10)
KasI 1291 GGCGCC G^GCGCC
KspI 185 CCGCGG CCGC^GG
MspA1I 185 CCGCGG C[AC]G^C[GT]G
NarI 1291 GGCGCC GG^CGCC
NdeI 455 CATATG CA^TATG
PflFI 1169 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 485 CCATGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 3416 AACGTT AA^CGTT
SacII 185 CCGCGG CCGC^GG
ScaI 1697 AGTACT AGT^ACT
SexAI 1347 ACCAGGT A^CC[AT]GGT
SfoI 1291 GGCGCC GGC^GCC
SmaI 126 CCCGGG CCC^GGG
SphI 3264 GCATGC GCATG^C
SspBI 854 TGTACA T^GTACA
StuI 94 AGGCCT AGG^CCT
TaqI 1368 TCGA T^CGA
Tth111I 1169 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1368 TCGA T^CGA
Van91I 485 CCATGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 126 CCCGGG C^CCGGG
XmaI 126 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccII 186 CGCG CG^CG
206 CGCG
AflII 1070 CTTAAG C^TTAAG
2513 CTTAAG
AflIII 662 ACATGT A^C[GA][CT]GT
2418 ACATGT
AlwNI 694 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
2557 CAGGCACTG
Asp700I 753 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3791 GAAATTTTTC
AvaII 495 GGTCC G^G[AT]CC
3729 GGACC
BanI 37 GGTGCC G^G[CT][GA]CC
1291 GGCGCC
BanII 58 GGGCCC G[GA]GC[CT]^C
216 GGGCTC
BfrI 1070 CTTAAG C^TTAAG
2513 CTTAAG
BlpI 155 GCTGAGC GC^T[ACGT]AGC
1771 GCTGAGC
BpmI 3498 CTCCAG CTGGAG(16/14)
1195 CTGGAG
Bpu1102I 155 GCTGAGC GC^T[ACGT]AGC
1771 GCTGAGC
BsaAI 2277 CACGTA [CT]AC^GT[GA]
3596 CACGTA
BsiYI 102 CCCGAGGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
485 CCATGCATTGG
BslI 102 CCCGAGGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
485 CCATGCATTGG
BsmBI 1167 GAGACG CGTCTC(1/5)
1334 GAGACG
BsmI 2895 GCATTC GAATGC(1/-1)
3834 GCATTC
BspLU11I 662 ACATGT A^CATGT
2418 ACATGT
BsrFI 160 GCCGGC [GA]^CCGG[CT]
252 GCCGGC
BstUI 186 CGCG CG^CG
206 CGCG
BstYI 2234 AGATCC [GA]^GATC[CT]
2309 AGATCC
CelII 155 GCTGAGC GC^T[ACGT]AGC
1771 GCTGAGC
Cfr10I 160 GCCGGC [GA]^CCGG[CT]
252 GCCGGC
DsaI 165 CCGTGG C^C[GA][CT]GG
185 CCGCGG
EcoT14I 3338 CCTAGG C^C[AT][AT]GG
3780 CCAAGG
HindIII 3166 AAGCTT A^AGCTT
3207 AAGCTT
MflI 2234 AGATCC [GA]^GATC[CT]
2309 AGATCC
MslI 2852 CACCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3217 CATTACAGTG
MvnI 186 CGCG CG^CG
206 CGCG
NaeI 160 GCCGGC GCC^GGC
252 GCCGGC
NgoMIV 160 GCCGGC G^CCGGC
252 GCCGGC
PstI 396 CTGCAG CTGCA^G
1060 CTGCAG
SfaNI 3802 GATGC GCATC(5/9)
402 GCATC
SpeI 2654 ACTAGT A^CTAGT
3380 ACTAGT
StyI 3338 CCTAGG C^C[AT][AT]GG
3780 CCAAGG
XhoII 2234 AGATCC [GA]^GATC[CT]
2309 AGATCC
XmnI 753 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3791 GAAATTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI ApoI AvaI BbsI
BbvCI BbvI BcnI BfaI BmyI
BpuAI BsaHI BsaI BsaJI BsiEI
BsmAI BsmFI BsoBI Bsp1286I BsrDI
BsrI BssKI Bst1107I BstF5I BstNI
BstXI BstZ17I Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EaeI
EagI EarI EclXI Eco52I EcoO109I
EcoRII EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HincII HindII HinfI HpaI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NsiI NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfcI SmlI SspI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XcmI