##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AscI AseI AsnI Asp718I
AvrII BbvCI BcgI BfrI BlnI
BsaBI BseAI BsiWI BspDI BspEI
BspLU11I BsrBI BssHII Bst1107I BstBI
BstXI BstZ17I ClaI CpoI DraI
DrdI EcoRV EcoT22I FseI HindIII
HpaI KpnI MamI MfeI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SalI SbfI SfuI SnaBI SpeI
Sse8387I SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 1952 GACGTC GACGT^C
AflIII 682 ACGCGT A^C[GA][CT]GT
AhdI 3604 GACCAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1224 AGCGCT AGC^GCT
ApaI 2124 GGGCCC GGGCC^C
AspEI 3604 GACCAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1952 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 2150 GGCGCC GGCGC^C
BbrPI 1307 CACGTG CAC^GTG
BbsI 1177 GAAGAC GAAGAC(2/6)
BciVI 907 GTATCC GTATCC(6/5)
BclI 1923 TGATCA T^GATCA
BlpI 3337 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 3337 GCTTAGC GC^T[ACGT]AGC
BpuAI 1177 GAAGAC GAAGAC(2/6)
BsaAI 1307 CACGTG [CT]AC^GT[GA]
BsaI 1845 GAGACC GGTCTC(1/5)
BsiEI 2043 CGGCCG CG[GA][CT]^CG
BsmBI 1049 GAGACG CGTCTC(1/5)
BsmI 385 GAATGC GAATGC(1/-1)
BspHI 1963 TCATGA T^CATGA
CelII 3337 GCTTAGC GC^T[ACGT]AGC
EagI 2043 CGGCCG C^GGCCG
Eam1105I 3604 GACCAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2043 CGGCCG C^GGCCG
Eco47III 1224 AGCGCT AGC^GCT
Eco52I 2043 CGGCCG C^GGCCG
EcoRI 332 GAATTC G^AATTC
FbaI 1923 TGATCA T^GATCA
HgaI 681 GACGC GACGC(5/10)
KasI 2150 GGCGCC G^GCGCC
KspI 1259 CCGCGG CCGC^GG
MluI 682 ACGCGT A^CGCGT
NaeI 59 GCCGGC GCC^GGC
NarI 2150 GGCGCC GG^CGCC
NgoMIV 59 GCCGGC G^CCGGC
PaeR7I 434 CTCGAG C^TCGAG
PflFI 1952 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1307 CACGTG CAC^GTG
PmlI 1307 CACGTG CAC^GTG
RcaI 1963 TCATGA T^CATGA
SacI 1112 GAGCTC GAGCT^C
SacII 1259 CCGCGG CCGC^GG
SapI 154 GAAGAGC GCTCTTC(1/4)
SexAI 3174 ACCTGGT A^CC[AT]GGT
SfoI 2150 GGCGCC GGC^GCC
SphI 3794 GCATGC GCATG^C
Tth111I 1952 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 434 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 332 GAATTC [GA]^AATT[CT]
554 GAATTT
AcyI 1952 GACGTC G[GA]^CG[CT]C
2150 GGCGCC
Alw44I 668 GTGCAC G^TGCAC
3869 GTGCAC
ApaLI 668 GTGCAC G^TGCAC
3869 GTGCAC
ApoI 332 GAATTC [GA]^AATT[CT]
554 GAATTT
Asp700I 154 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2188 GAACCTCTTC
AviII 1344 TGCGCA TGC^GCA
3861 TGCGCA
BglI 2168 GCCGAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3998 GCCACTGGGGC
BglII 2064 AGATCT A^GATCT
2538 AGATCT
BsaHI 1952 GACGTC G[GA]^CG[CT]C
2150 GGCGCC
BsgI 1811 CTGCAC GTGCAG(16/14)
3170 CTGCAC
Bsp1407I 1827 TGTACA T^GTACA
3535 TGTACA
BsrDI 3721 CATTGC GCAATG(2/0)
198 GCAATG
BsrGI 1827 TGTACA T^GTACA
3535 TGTACA
BssSI 1781 CACGAG CACGAG(-5/-1)
257 CTCGTG
BstAPI 238 GCACACGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2600 GCACACCCTGC
BstEII 1904 GGTTACC G^GT[ACGT]ACC
1936 GGTCACC
BstPI 1904 GGTTACC G^GT[ACGT]ACC
1936 GGTCACC
EcoNI 1714 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3308 CCTTGGTCAGG
EcoO65I 1904 GGTTACC G^GT[ACGT]ACC
1936 GGTCACC
FspI 1344 TGCGCA TGC^GCA
3861 TGCGCA
Hin1I 1952 GACGTC G[GA]^CG[CT]C
2150 GGCGCC
HincII 782 GTCAAC GT[CT]^[GA]AC
2815 GTTGAC
HindII 782 GTCAAC GT[CT]^[GA]AC
2815 GTTGAC
NcoI 2142 CCATGG C^CATGG
2729 CCATGG
ScaI 957 AGTACT AGT^ACT
2259 AGTACT
SfiI 2167 GGCCGAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3997 GGCCACTGGGGCC
SgrAI 58 CGCCGGCG C[GA]^CCGG[CT]G
1535 CGCCGGTG
SspBI 1827 TGTACA T^GTACA
3535 TGTACA
XmnI 154 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2188 GAACCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI AspHI AvaI AvaII
BalI BamHI BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI