################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AgeI AscI AseI AsnI Asp718I AvrII BbvCI BcgI BfrI BlnI BsaBI BseAI BsiWI BspDI BspEI BspLU11I BsrBI BssHII Bst1107I BstBI BstXI BstZ17I ClaI CpoI DraI DrdI EcoRV EcoT22I FseI HindIII HpaI KpnI MamI MfeI MroI MunI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI SbfI SfuI SnaBI SpeI Sse8387I SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 1952 GACGTC GACGT^C AflIII 682 ACGCGT A^C[GA][CT]GT AhdI 3604 GACCAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 1224 AGCGCT AGC^GCT ApaI 2124 GGGCCC GGGCC^C AspEI 3604 GACCAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1952 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 2150 GGCGCC GGCGC^C BbrPI 1307 CACGTG CAC^GTG BbsI 1177 GAAGAC GAAGAC(2/6) BciVI 907 GTATCC GTATCC(6/5) BclI 1923 TGATCA T^GATCA BlpI 3337 GCTTAGC GC^T[ACGT]AGC Bpu1102I 3337 GCTTAGC GC^T[ACGT]AGC BpuAI 1177 GAAGAC GAAGAC(2/6) BsaAI 1307 CACGTG [CT]AC^GT[GA] BsaI 1845 GAGACC GGTCTC(1/5) BsiEI 2043 CGGCCG CG[GA][CT]^CG BsmBI 1049 GAGACG CGTCTC(1/5) BsmI 385 GAATGC GAATGC(1/-1) BspHI 1963 TCATGA T^CATGA CelII 3337 GCTTAGC GC^T[ACGT]AGC EagI 2043 CGGCCG C^GGCCG Eam1105I 3604 GACCAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 2043 CGGCCG C^GGCCG Eco47III 1224 AGCGCT AGC^GCT Eco52I 2043 CGGCCG C^GGCCG EcoRI 332 GAATTC G^AATTC FbaI 1923 TGATCA T^GATCA HgaI 681 GACGC GACGC(5/10) KasI 2150 GGCGCC G^GCGCC KspI 1259 CCGCGG CCGC^GG MluI 682 ACGCGT A^CGCGT NaeI 59 GCCGGC GCC^GGC NarI 2150 GGCGCC GG^CGCC NgoMIV 59 GCCGGC G^CCGGC PaeR7I 434 CTCGAG C^TCGAG PflFI 1952 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1307 CACGTG CAC^GTG PmlI 1307 CACGTG CAC^GTG RcaI 1963 TCATGA T^CATGA SacI 1112 GAGCTC GAGCT^C SacII 1259 CCGCGG CCGC^GG SapI 154 GAAGAGC GCTCTTC(1/4) SexAI 3174 ACCTGGT A^CC[AT]GGT SfoI 2150 GGCGCC GGC^GCC SphI 3794 GCATGC GCATG^C Tth111I 1952 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 434 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcsI 332 GAATTC [GA]^AATT[CT] 554 GAATTT AcyI 1952 GACGTC G[GA]^CG[CT]C 2150 GGCGCC Alw44I 668 GTGCAC G^TGCAC 3869 GTGCAC ApaLI 668 GTGCAC G^TGCAC 3869 GTGCAC ApoI 332 GAATTC [GA]^AATT[CT] 554 GAATTT Asp700I 154 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2188 GAACCTCTTC AviII 1344 TGCGCA TGC^GCA 3861 TGCGCA BglI 2168 GCCGAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3998 GCCACTGGGGC BglII 2064 AGATCT A^GATCT 2538 AGATCT BsaHI 1952 GACGTC G[GA]^CG[CT]C 2150 GGCGCC BsgI 1811 CTGCAC GTGCAG(16/14) 3170 CTGCAC Bsp1407I 1827 TGTACA T^GTACA 3535 TGTACA BsrDI 3721 CATTGC GCAATG(2/0) 198 GCAATG BsrGI 1827 TGTACA T^GTACA 3535 TGTACA BssSI 1781 CACGAG CACGAG(-5/-1) 257 CTCGTG BstAPI 238 GCACACGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2600 GCACACCCTGC BstEII 1904 GGTTACC G^GT[ACGT]ACC 1936 GGTCACC BstPI 1904 GGTTACC G^GT[ACGT]ACC 1936 GGTCACC EcoNI 1714 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3308 CCTTGGTCAGG EcoO65I 1904 GGTTACC G^GT[ACGT]ACC 1936 GGTCACC FspI 1344 TGCGCA TGC^GCA 3861 TGCGCA Hin1I 1952 GACGTC G[GA]^CG[CT]C 2150 GGCGCC HincII 782 GTCAAC GT[CT]^[GA]AC 2815 GTTGAC HindII 782 GTCAAC GT[CT]^[GA]AC 2815 GTTGAC NcoI 2142 CCATGG C^CATGG 2729 CCATGG ScaI 957 AGTACT AGT^ACT 2259 AGTACT SfiI 2167 GGCCGAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 3997 GGCCACTGGGGCC SgrAI 58 CGCCGGCG C[GA]^CCGG[CT]G 1535 CGCCGGTG SspBI 1827 TGTACA T^GTACA 3535 TGTACA XmnI 154 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2188 GAACCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 115 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AocI AspHI AvaI AvaII BalI BamHI BanI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI