##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspHI AvaI AviII AvrII
BanI BanII BbeI BbrPI BbvCI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BseAI BsiHKAI BsiWI
BsmBI BsoBI BspDI BspEI BspLU11I
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I CelII
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV FseI FspI HaeII Hin1I
HpaI KasI KpnI KspI MfeI
MluI MroI MunI MvnI NaeI
NarI NgoMIV NotI NruI NspV
PacI PaeR7I PflMI PmaCI PmeI
PmlI PshAI Psp1406I RsrII SacI
SacII SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI Van91I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 1403 GTCGAC GT^[AC][GT]AC
AgeI 871 ACCGGT A^CCGGT
AocI 243 CCTGAGG CC^T[ACGT]AGG
Asp700I 607 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1437 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1168 TGGCCA TGG^CCA
BcnI 920 CCGGG CC^[GC]GG
BsaBI 2606 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 871 ACCGGT [AT]^CCGG[AT]
BseRI 3233 CTCCTC GAGGAG(10/8)
BsiEI 1805 CGATCG CG[GA][CT]^CG
Bsp1407I 3206 TGTACA T^GTACA
BsrBI 1932 GAGCGG CCGCTC(-3/-3)
BsrFI 871 ACCGGT [GA]^CCGG[CT]
BsrGI 3206 TGTACA T^GTACA
Bsu36I 243 CCTGAGG CC^T[ACGT]AGG
Cfr10I 871 ACCGGT [GA]^CCGG[CT]
DraI 2793 TTTAAA TTT^AAA
EaeI 1168 TGGCCA [CT]^GGCC[GA]
Eco81I 243 CCTGAGG CC^T[ACGT]AGG
FauI 1934 GCGGG CCCGC(4/6)
MamI 2606 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1168 TGGCCA TGG^CCA
MscI 1168 TGGCCA TGG^CCA
NciI 920 CCGGG CC^[GC]GG
NcoI 2438 CCATGG C^CATGG
NheI 181 GCTAGC G^CTAGC
PflFI 1437 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 871 ACCGGT A^CCGGT
PstI 131 CTGCAG CTGCA^G
PvuI 1805 CGATCG CGAT^CG
SalI 1403 GTCGAC G^TCGAC
SapI 493 GCTCTTC GCTCTTC(1/4)
ScaI 3061 AGTACT AGT^ACT
SexAI 961 ACCAGGT A^CC[AT]GGT
SphI 2408 GCATGC GCATG^C
SspBI 3206 TGTACA T^GTACA
SspI 3623 AATATT AAT^ATT
StuI 4004 AGGCCT AGG^CCT
Tth111I 1437 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1858 CCACCAAGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 607 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AseI 1094 ATTAAT AT^TAAT
3374 ATTAAT
AsnI 1094 ATTAAT AT^TAAT
3374 ATTAAT
BamHI 479 GGATCC G^GATCC
3761 GGATCC
BciVI 2357 GGATAC GTATCC(6/5)
2965 GGATAC
BclI 1499 TGATCA T^GATCA
2611 TGATCA
BglI 2557 GCCTTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3515 GCCCCCTTGGC
BmyI 531 GGGCAC G[AGT]GC[ACT]^C
3167 GTGCCC
BsaI 855 GAGACC GGTCTC(1/5)
1279 GAGACC
Bsp1286I 531 GGGCAC G[AGT]GC[ACT]^C
3167 GTGCCC
BspHI 1066 TCATGA T^CATGA
1639 TCATGA
BspMI 129 ACCTGC ACCTGC(4/8)
2452 ACCTGC
CfoI 542 GCGC GCG^C
3300 GCGC
EcoNI 456 CCTTCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1166 CCTGGCCAAGG
FbaI 1499 TGATCA T^GATCA
2611 TGATCA
HapII 872 CCGG C^CGG
920 CCGG
HhaI 542 GCGC GCG^C
3300 GCGC
HinP1I 542 GCGC G^CGC
3300 GCGC
HindIII 2568 AAGCTT A^AGCTT
2844 AAGCTT
HpaII 872 CCGG C^CGG
920 CCGG
MspI 872 CCGG C^CGG
920 CCGG
NdeI 1719 CATATG CA^TATG
3509 CATATG
PshBI 1094 ATTAAT AT^TAAT
3374 ATTAAT
PvuII 748 CAGCTG CAG^CTG
1480 CAGCTG
RcaI 1066 TCATGA T^CATGA
1639 TCATGA
SmlI 288 CTTGAG C^T[CT][GA]AG
2489 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BbsI BbvI
BcgI BfaI BpmI BpuAI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
BsmI BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraII DrdI DsaI
EarI EcoO109I EcoRII EcoT14I EcoT22I
Fnu4HI FokI HaeIII HgaI HincII
HindII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NsiI NspI PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII