##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp718I AspEI BbeI BbrPI
BbvCI BciVI BclI BfrI BsaAI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssHII BstBI
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoRV EcoT22I
FbaI FseI HincII HindII HpaI
KasI KpnI KspI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AatII 75 GACGTC GACGT^C
AccI 3260 GTATAC GT^[AC][GT]AC
AcsI 2174 GAATTC [GA]^AATT[CT]
Alw44I 609 GTGCAC G^TGCAC
ApaLI 609 GTGCAC G^TGCAC
ApoI 2174 GAATTC [GA]^AATT[CT]
Asp700I 1417 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 3600 CCTAGG C^CTAGG
BamHI 3876 GGATCC G^GATCC
BglII 2112 AGATCT A^GATCT
BlnI 3600 CCTAGG C^CTAGG
BsaWI 1788 ACCGGA [AT]^CCGG[AT]
BspHI 2788 TCATGA T^CATGA
BssSI 2580 CACGAG CACGAG(-5/-1)
Bst1107I 3260 GTATAC GTA^TAC
BstZ17I 3260 GTATAC GTA^TAC
DrdI 1700 GACCTAAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1078 CCTGCGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2174 GAATTC G^AATTC
HindIII 1943 AAGCTT A^AGCTT
NheI 3343 GCTAGC G^CTAGC
PstI 2129 CTGCAG CTGCA^G
RcaI 2788 TCATGA T^CATGA
SacI 169 GAGCTC GAGCT^C
ScaI 3150 AGTACT AGT^ACT
SspI 4078 AATATT AAT^ATT
XmnI 1417 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AspI 1892 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1963 GACCTTGTC
AviII 315 TGCGCA TGC^GCA
1080 TGCGCA
BcgI 336 GCAGCCTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
439 GCAGGCGAATCG
BlpI 685 GCTCAGC GC^T[ACGT]AGC
1024 GCTGAGC
Bpu1102I 685 GCTCAGC GC^T[ACGT]AGC
1024 GCTGAGC
BsaBI 3575 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3877 GATCCTAATC
BsmI 2413 GAATGC GAATGC(1/-1)
158 GCATTC
BsrDI 1723 CATTGC GCAATG(2/0)
2614 CATTGC
BstAPI 1388 GCAGTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2557 GCAGGAGCTGC
BstEII 2260 GGTCACC G^GT[ACGT]ACC
2467 GGTCACC
BstPI 2260 GGTCACC G^GT[ACGT]ACC
2467 GGTCACC
CelII 685 GCTCAGC GC^T[ACGT]AGC
1024 GCTGAGC
EcoO65I 2260 GGTCACC G^GT[ACGT]ACC
2467 GGTCACC
FspI 315 TGCGCA TGC^GCA
1080 TGCGCA
MamI 3575 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3877 GATCCTAATC
MseI 3948 TTAA T^TAA
4072 TTAA
NspI 2982 GCATGT [GA]CATG^[CT]
3109 GCATGT
PflFI 1892 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1963 GACCTTGTC
SmaI 2157 CCCGGG CCC^GGG
3042 CCCGGG
StuI 457 AGGCCT AGG^CCT
1857 AGGCCT
Tru9I 3948 TTAA T^TAA
4072 TTAA
Tth111I 1892 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1963 GACCTTGTC
XmaCI 2157 CCCGGG C^CCGGG
3042 CCCGGG
XmaI 2157 CCCGGG C^CCGGG
3042 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII