##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AccIII AgeI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I AviII
AvrII BalI BglII BlnI BsaWI
BseAI BsiEI BsiWI Bsp1407I BspEI
BsrGI BssHII BstBI BstEII BstPI
Bsu36I CpoI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV FseI
FspI MluI MluNI MroI MscI
NcoI NdeI NheI NotI NruI
NspV PacI PinAI PmeI PshBI
PvuI RsrII SalI SapI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SspBI SwaI XcmI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AatII 2943 GACGTC GACGT^C
AccI 1541 GTATAC GT^[AC][GT]AC
AclI 180 AACGTT AA^CGTT
AflII 3160 CTTAAG C^TTAAG
AhdI 165 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 133 GTGCAC G^TGCAC
ApaLI 133 GTGCAC G^TGCAC
AspEI 165 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2110 CTCGAG C^[CT]CG[GA]G
BamHI 208 GGATCC G^GATCC
BbrPI 2870 CACGTG CAC^GTG
BciVI 863 GGATAC GTATCC(6/5)
BfrI 3160 CTTAAG C^TTAAG
BglI 9 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 443 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 443 GCTGAGC GC^T[ACGT]AGC
BsaAI 2870 CACGTG [CT]AC^GT[GA]
BsaBI 2379 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 2555 GAGACG CGTCTC(1/5)
BsoBI 2110 CTCGAG C^[CT]CG[GA]G
BspHI 1643 TCATGA T^CATGA
BsrFI 18 GCCGGC [GA]^CCGG[CT]
Bst1107I 1541 GTATAC GTA^TAC
BstXI 3742 CCAAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1541 GTATAC GTA^TAC
CelII 443 GCTGAGC GC^T[ACGT]AGC
Cfr10I 18 GCCGGC [GA]^CCGG[CT]
DrdI 1017 GACACAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 84 CCGCGG C^C[GA][CT]GG
EaeI 16 TGGCCG [CT]^GGCC[GA]
Eam1105I 165 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 449 CCTCAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1948 GAATTC G^AATTC
EcoT14I 1316 CCAAGG C^C[AT][AT]GG
HgaI 1704 GACGC GACGC(5/10)
HindIII 3163 AAGCTT A^AGCTT
HpaI 3303 GTTAAC GTT^AAC
KspI 84 CCGCGG CCGC^GG
MamI 2379 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2092 CAATTG C^AATTG
MslI 947 CATCAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 2092 CAATTG C^AATTG
NaeI 18 GCCGGC GCC^GGC
NgoMIV 18 GCCGGC G^CCGGC
PaeR7I 2110 CTCGAG C^TCGAG
PflMI 2375 CCAGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2870 CACGTG CAC^GTG
PmlI 2870 CACGTG CAC^GTG
PshAI 2760 GACGAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 180 AACGTT AA^CGTT
PvuII 1345 CAGCTG CAG^CTG
RcaI 1643 TCATGA T^CATGA
SacI 608 GAGCTC GAGCT^C
SacII 84 CCGCGG CCGC^GG
SbfI 2948 CCTGCAGG CCTGCA^GG
SphI 2960 GCATGC GCATG^C
Sse8387I 2948 CCTGCAGG CCTGCA^GG
StyI 1316 CCAAGG C^C[AT][AT]GG
Van91I 2375 CCAGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1952 TCTAGA T^CTAGA
XhoI 2110 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 1843 GGTACC G^GTACC
2844 GGTACC
Asp718I 1843 GGTACC G^GTACC
2844 GGTACC
AspI 842 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1679 GACCAAGTC
BanII 608 GAGCTC G[GA]GC[CT]^C
2820 GAGCCC
BcgI 178 CGAACGTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
271 GCAGCAACTTCG
BclI 1646 TGATCA T^GATCA
2489 TGATCA
BspDI 1159 ATCGAT AT^CGAT
1556 ATCGAT
BspLU11I 2203 ACATGT A^CATGT
3045 ACATGT
BspMI 1846 ACCTGC ACCTGC(4/8)
2967 GCAGGT
BsrBI 1094 CCGCTC CCGCTC(-3/-3)
3012 GAGCGG
BstAPI 357 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2951 GCAGGAACTGC
ClaI 1159 ATCGAT AT^CGAT
1556 ATCGAT
DraII 457 AGGACCC [GA]G^G[ACGT]CC[CT]
1597 AGGTCCT
DraIII 2403 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
3032 CACGTCGTG
EcoO109I 457 AGGACCC [GA]G^G[ACGT]CC[CT]
1597 AGGTCCT
FbaI 1646 TGATCA T^GATCA
2489 TGATCA
HincII 2082 GTTGAC GT[CT]^[GA]AC
3303 GTTAAC
HindII 2082 GTTGAC GT[CT]^[GA]AC
3303 GTTAAC
KpnI 1843 GGTACC GGTAC^C
2844 GGTACC
PflFI 842 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1679 GACCAAGTC
PpuMI 457 AGGACCC [GA]G^G[AT]CC[CT]
1597 AGGTCCT
StuI 511 AGGCCT AGG^CCT
555 AGGCCT
TfiI 1562 GATTC G^A[AT]TC
2664 GAATC
Tth111I 842 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1679 GACCAAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaII BanI BbeI BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrDI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI EarI EcoRII
EcoT22I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NsiI
NspI PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI SspI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII