##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AspEI AspI
AvaI AviII AvrII BbeI BbrPI
BbvCI BcgI BglI BlnI BlpI
Bpu1102I BsaAI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI BspEI
BsrBI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I CpoI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HpaI KasI KspI MaeII
MluI MroI MvnI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I StuI SwaI
Tth111I XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 1039 GGTACC G^GTACC
Asp718I 1039 GGTACC G^GTACC
BalI 2659 TGGCCA TGG^CCA
BamHI 3112 GGATCC G^GATCC
BciVI 3557 GGATAC GTATCC(6/5)
BsaI 783 GAGACC GGTCTC(1/5)
BsmFI 2370 GTCCC GGGAC(10/14)
BspHI 42 TCATGA T^CATGA
BspMI 1048 GCAGGT ACCTGC(4/8)
BsrDI 3443 GCAATG GCAATG(2/0)
BstXI 2374 CCAAGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 1789 AGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 265 GACATCTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1789 AGGTCCT [GA]G^G[ACGT]CC[CT]
FauI 1528 GCGGG CCCGC(4/6)
KpnI 1039 GGTACC GGTAC^C
MfeI 568 CAATTG C^AATTG
MluNI 2659 TGGCCA TGG^CCA
MscI 2659 TGGCCA TGG^CCA
MunI 568 CAATTG C^AATTG
PflMI 1761 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1789 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 42 TCATGA T^CATGA
SphI 4066 GCATGC GCATG^C
Van91I 1761 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 1124 GTCGAC GT^[AC][GT]AC
1994 GTCGAC
AflII 2483 CTTAAG C^TTAAG
3182 CTTAAG
AflIII 1096 ACATGT A^C[GA][CT]GT
2833 ACATGT
BanI 1039 GGTACC G^G[CT][GA]CC
2931 GGCACC
BanII 192 GAGCTC G[GA]GC[CT]^C
2567 GAGCTC
BcnI 115 CCGGG CC^[GC]GG
3034 CCGGG
BfrI 2483 CTTAAG C^TTAAG
3182 CTTAAG
BglII 3085 AGATCT A^GATCT
3586 AGATCT
BpmI 1197 CTGGAG CTGGAG(16/14)
1500 CTGGAG
BsaBI 992 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4285 GATAAGAATC
BseRI 1148 GAGGAG GAGGAG(10/8)
1744 GAGGAG
BsmI 1521 GAATGC GAATGC(1/-1)
3573 GCATTC
Bsp1407I 1916 TGTACA T^GTACA
4388 TGTACA
BspDI 2 ATCGAT AT^CGAT
776 ATCGAT
BspLU11I 1096 ACATGT A^CATGT
2833 ACATGT
BsrGI 1916 TGTACA T^GTACA
4388 TGTACA
BstAPI 3573 GCATTCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4474 GCATTTATTGC
ClaI 2 ATCGAT AT^CGAT
776 ATCGAT
DsaI 500 CCATGG C^C[GA][CT]GG
883 CCATGG
EaeI 2157 TGGCCG [CT]^GGCC[GA]
2659 TGGCCA
EcoT14I 500 CCATGG C^C[AT][AT]GG
883 CCATGG
HapII 115 CCGG C^CGG
3034 CCGG
HincII 1124 GTCGAC GT[CT]^[GA]AC
1994 GTCGAC
HindII 1124 GTCGAC GT[CT]^[GA]AC
1994 GTCGAC
HindIII 535 AAGCTT A^AGCTT
3044 AAGCTT
HpaII 115 CCGG C^CGG
3034 CCGG
MamI 992 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4285 GATAAGAATC
MspI 115 CCGG C^CGG
3034 CCGG
NciI 115 CCGGG CC^[GC]GG
3034 CCGGG
NcoI 500 CCATGG C^CATGG
883 CCATGG
SacI 192 GAGCTC GAGCT^C
2567 GAGCTC
SalI 1124 GTCGAC G^TCGAC
1994 GTCGAC
ScaI 1720 AGTACT AGT^ACT
3508 AGTACT
SspBI 1916 TGTACA T^GTACA
4388 TGTACA
StyI 500 CCATGG C^C[AT][AT]GG
883 CCATGG
XbaI 713 TCTAGA T^CTAGA
3803 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI Asp700I
AspHI AvaII BbsI BbvI BclI
BfaI BmyI BpuAI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
EarI EcoRI EcoRII EcoRV EcoT22I
FbaI Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NdeI
NdeII NlaIII NlaIV NsiI NspI
PleI PshBI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI