##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AclI AflII AscI AseI
AsnI AspI AviII AvrII BalI
BbrPI BbvCI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsiWI BspDI
BssHII BstBI BstEII BstPI CelII
ClaI CpoI DrdI EcoO65I EcoRI
FspI HincII HindII HpaI MamI
MfeI MluI MluNI MscI MunI
NdeI NheI NotI NspV PacI
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SalI
SbfI ScaI SexAI SfuI SnaBI
SpeI Sse8387I SspI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 2328 GGTACC G^GTACC
AccIII 225 TCCGGA T^CCGGA
AhdI 3343 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1243 GTGCAC G^TGCAC
Aor51HI 3373 AGCGCT AGC^GCT
ApaLI 1243 GTGCAC G^TGCAC
Asp718I 2328 GGTACC G^GTACC
AspEI 3343 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1412 GGATCC G^GATCC
BciVI 3468 GGATAC GTATCC(6/5)
BglII 1073 AGATCT A^GATCT
BseAI 225 TCCGGA T^CCGGA
BsmI 2733 GAATGC GAATGC(1/-1)
Bsp1407I 4158 TGTACA T^GTACA
BspEI 225 TCCGGA T^CCGGA
BsrGI 4158 TGTACA T^GTACA
Bst1107I 3304 GTATAC GTA^TAC
BstZ17I 3304 GTATAC GTA^TAC
DraI 4457 TTTAAA TTT^AAA
EagI 98 CGGCCG C^GGCCG
Eam1105I 3343 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 98 CGGCCG C^GGCCG
Eco47III 3373 AGCGCT AGC^GCT
Eco52I 98 CGGCCG C^GGCCG
EcoRV 1021 GATATC GAT^ATC
EcoT22I 2584 ATGCAT ATGCA^T
FseI 3070 GGCCGGCC GGCCGG^CC
HindIII 2809 AAGCTT A^AGCTT
KpnI 2328 GGTACC GGTAC^C
KspI 3513 CCGCGG CCGC^GG
MroI 225 TCCGGA T^CCGGA
NruI 83 TCGCGA TCG^CGA
NsiI 2584 ATGCAT ATGCA^T
SacII 3513 CCGCGG CCGC^GG
SfiI 3065 GGCCGGGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 124 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 164 CCCGGG CCC^GGG
SphI 2582 GCATGC GCATG^C
SspBI 4158 TGTACA T^GTACA
SwaI 4456 ATTTAAAT ATTT^AAAT
XmaCI 164 CCCGGG C^CCGGG
XmaI 164 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 2409 GAATTT [GA]^AATT[CT]
4454 GAATTT
AflIII 1226 ACATGT A^C[GA][CT]GT
2975 ACATGT
AgeI 941 ACCGGT A^CCGGT
4361 ACCGGT
ApoI 2409 GAATTT [GA]^AATT[CT]
4454 GAATTT
Asp700I 366 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3291 GAAGAGTTTC
BclI 2777 TGATCA T^GATCA
3650 TGATCA
BsiEI 98 CGGCCG CG[GA][CT]^CG
4363 CGGTCG
BspHI 1025 TCATGA T^CATGA
3149 TCATGA
BspLU11I 1226 ACATGT A^CATGT
2975 ACATGT
BstXI 1480 CCAGCTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1587 CCAGCAGGCTGG
DraIII 2762 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
4406 CACTAGGTG
FbaI 2777 TGATCA T^GATCA
3650 TGATCA
MaeII 2016 ACGT A^CGT
2835 ACGT
MseI 2096 TTAA T^TAA
4458 TTAA
NaeI 125 GCCGGC GCC^GGC
3071 GCCGGC
NgoMIV 125 GCCGGC G^CCGGC
3071 GCCGGC
PaeR7I 323 CTCGAG C^TCGAG
2862 CTCGAG
PinAI 941 ACCGGT A^CCGGT
4361 ACCGGT
RcaI 1025 TCATGA T^CATGA
3149 TCATGA
SapI 1941 GAAGAGC GCTCTTC(1/4)
2647 GAAGAGC
Tru9I 2096 TTAA T^TAA
4458 TTAA
XhoI 323 CTCGAG C^TCGAG
2862 CTCGAG
XmnI 366 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3291 GAAGAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BanI BanII
BbeI BbsI BbvI BcgI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI StuI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII