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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII AccII AccIII AcyI AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AspEI AviII AvrII BamHI BbeI
BbrPI BcgI BcnI BglI BglII
BlnI BsaAI BsaHI BseAI BsiWI
BsmBI BspDI BspEI BspHI BspMI
BssHII BssSI BstBI BstEII BstPI
BstUI CfoI ClaI CpoI DrdI
DsaI Eam1105I Eco47III EcoNI EcoO65I
EcoT14I FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HpaI KasI
KspI MfeI MluI MroI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I StuI
StyI Van91I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
Acc65I 2652 GGTACC G^GTACC
AccI 3064 GTATAC GT^[AC][GT]AC
AclI 2853 AACGTT AA^CGTT
AflIII 2960 ACATGT A^C[GA][CT]GT
AlwNI 1601 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 3522 ATTAAT AT^TAAT
AsnI 3522 ATTAAT AT^TAAT
Asp718I 2652 GGTACC G^GTACC
AspHI 3536 GTGCTC G[AT]GC[AT]^C
AspI 2817 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2800 CTCGGG C^[CT]CG[GA]G
BalI 5 TGGCCA TGG^CCA
BbsI 1809 GTCTTC GAAGAC(2/6)
BciVI 1012 GTATCC GTATCC(6/5)
BclI 606 TGATCA T^GATCA
BlpI 2196 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2196 GCTCAGC GC^T[ACGT]AGC
BpuAI 1809 GTCTTC GAAGAC(2/6)
BsaBI 2270 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2815 GAGACC GGTCTC(1/5)
BsaJI 1373 CCCAGG C^C[ACGT][ACGT]GG
BsaWI 25 TCCGGT [AT]^CCGG[AT]
BseRI 1577 CTCCTC GAGGAG(10/8)
BsiEI 656 CGGCCG CG[GA][CT]^CG
BsiHKAI 3536 GTGCTC G[AT]GC[AT]^C
BsoBI 2800 CTCGGG C^[CT]CG[GA]G
Bsp1407I 2429 TGTACA T^GTACA
BspLU11I 2960 ACATGT A^CATGT
BsrBI 406 GAGCGG CCGCTC(-3/-3)
BsrDI 4007 GCAATG GCAATG(2/0)
BsrFI 658 GCCGGT [GA]^CCGG[CT]
BsrGI 2429 TGTACA T^GTACA
Bst1107I 3064 GTATAC GTA^TAC
BstAPI 2005 GCAACCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 3064 GTATAC GTA^TAC
CelII 2196 GCTCAGC GC^T[ACGT]AGC
Cfr10I 658 GCCGGT [GA]^CCGG[CT]
DraII 2598 AGGACCC [GA]G^G[ACGT]CC[CT]
EagI 656 CGGCCG C^GGCCG
EclXI 656 CGGCCG C^GGCCG
Eco52I 656 CGGCCG C^GGCCG
EcoO109I 2598 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 1798 GATATC GAT^ATC
FauI 2602 CCCGC CCCGC(4/6)
FbaI 606 TGATCA T^GATCA
HincII 834 GTCAAC GT[CT]^[GA]AC
HindII 834 GTCAAC GT[CT]^[GA]AC
KpnI 2652 GGTACC GGTAC^C
MamI 2270 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 5 TGGCCA TGG^CCA
MscI 5 TGGCCA TGG^CCA
NdeI 3449 CATATG CA^TATG
PflFI 2817 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2598 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 3522 ATTAAT AT^TAAT
Psp1406I 2853 AACGTT AA^CGTT
PvuII 3827 CAGCTG CAG^CTG
SpeI 1726 ACTAGT A^CTAGT
SspBI 2429 TGTACA T^GTACA
SwaI 3853 ATTTAAAT ATTT^AAAT
Tth111I 2817 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1925 CCAAAGAAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflII 732 CTTAAG C^TTAAG
3553 CTTAAG
AocI 2594 CCTCAGG CC^T[ACGT]AGG
3412 CCTTAGG
ApaI 703 GGGCCC GGGCC^C
3137 GGGCCC
BanI 661 GGTGCC G^G[CT][GA]CC
2652 GGTACC
BbvCI 187 GCTGAGG CCTCAGC(-5/-2)
2742 GCTGAGG
BfrI 732 CTTAAG C^TTAAG
3553 CTTAAG
BsgI 2789 CTGCAC GTGCAG(16/14)
1967 GTGCAG
BsmI 263 GAATGC GAATGC(1/-1)
469 GAATGC
BstXI 2580 CCACTTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2727 CCACTACTTTGG
Bsu36I 2594 CCTCAGG CC^T[ACGT]AGG
3412 CCTTAGG
EaeI 5 TGGCCA [CT]^GGCC[GA]
656 CGGCCG
Eco81I 2594 CCTCAGG CC^T[ACGT]AGG
3412 CCTTAGG
EcoRI 448 GAATTC G^AATTC
2569 GAATTC
EcoT22I 2537 ATGCAT ATGCA^T
3757 ATGCAT
HapII 26 CCGG C^CGG
659 CCGG
HindIII 2035 AAGCTT A^AGCTT
2940 AAGCTT
HpaII 26 CCGG C^CGG
659 CCGG
MaeII 2117 ACGT A^CGT
2854 ACGT
MspA1I 475 CAGCGG C[AC]G^C[GT]G
3827 CAGCTG
MspI 26 CCGG C^CGG
659 CCGG
NsiI 2537 ATGCAT ATGCA^T
3757 ATGCAT
SspI 3511 AATATT AAT^ATT
4036 AATATT
TaqI 1802 TCGA T^CGA
2304 TCGA
TthHB8I 1802 TCGA T^CGA
2304 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaII BanII BbvI
BfaI BmyI BpmI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraIII EarI
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SphI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII
XmnI