##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI AspEI AviII AvrII BamHI
BbeI BciVI BfrI BlnI BlpI
Bpu1102I BseAI Bsp1407I BspDI BspEI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstEII BstPI BstUI BstZ17I CelII
Cfr10I ClaI CpoI DraIII DrdI
Eam1105I Eco47III EcoO65I EcoRI FseI
FspI HaeII HindIII HpaI KasI
KspI MfeI MluI MroI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PaeR7I
PflMI PinAI PmeI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
ScaI SexAI SfoI SgrAI SmaI
SnaBI SpeI SspBI SwaI Van91I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 700 GGTACC G^GTACC
AccI 3149 GTCTAC GT^[AC][GT]AC
AcsI 3595 AAATTT [GA]^AATT[CT]
AcyI 4404 GGCGTC G[GA]^CG[CT]C
AocI 3645 CCTGAGG CC^T[ACGT]AGG
ApoI 3595 AAATTT [GA]^AATT[CT]
Asp718I 700 GGTACC G^GTACC
AvaI 918 CCCGAG C^[CT]CG[GA]G
BbrPI 1317 CACGTG CAC^GTG
BglII 1938 AGATCT A^GATCT
BsaBI 4017 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 4404 GGCGTC G[GA]^CG[CT]C
BsaWI 391 ACCGGA [AT]^CCGG[AT]
BsiEI 562 CGGCCG CG[GA][CT]^CG
BsiWI 1540 CGTACG C^GTACG
BsoBI 918 CCCGAG C^[CT]CG[GA]G
BspHI 4207 TCATGA T^CATGA
BspLU11I 3944 ACATGT A^CATGT
BsrBI 12 GAGCGG CCGCTC(-3/-3)
BssSI 2084 CTCGTG CACGAG(-5/-1)
BstBI 1658 TTCGAA TT^CGAA
Bsu36I 3645 CCTGAGG CC^T[ACGT]AGG
CfoI 156 GCGC GCG^C
DraI 3093 TTTAAA TTT^AAA
EagI 562 CGGCCG C^GGCCG
EclXI 562 CGGCCG C^GGCCG
Eco52I 562 CGGCCG C^GGCCG
Eco81I 3645 CCTGAGG CC^T[ACGT]AGG
EcoRV 3409 GATATC GAT^ATC
HhaI 156 GCGC GCG^C
Hin1I 4404 GGCGTC G[GA]^CG[CT]C
HinP1I 156 GCGC G^CGC
KpnI 700 GGTACC GGTAC^C
MamI 4017 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspV 1658 TTCGAA TT^CGAA
PmaCI 1317 CACGTG CAC^GTG
PmlI 1317 CACGTG CAC^GTG
PshAI 2360 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 4207 TCATGA T^CATGA
SfiI 650 GGCCACCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1658 TTCGAA TT^CGAA
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AflIII 3718 ACGTGT A^C[GA][CT]GT
3944 ACATGT
Alw44I 1320 GTGCAC G^TGCAC
1648 GTGCAC
ApaI 780 GGGCCC GGGCC^C
915 GGGCCC
ApaLI 1320 GTGCAC G^TGCAC
1648 GTGCAC
AspI 2128 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4417 GACCCCGTC
BcgI 3344 CGACATTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1650 GCACAGTTTTCG
BglI 651 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
753 GCCAGTGTGGC
BsaAI 1317 CACGTG [CT]AC^GT[GA]
2872 TACGTG
BsmBI 4255 CGTCTC CGTCTC(1/5)
4422 CGTCTC
BsrDI 2459 CATTGC GCAATG(2/0)
3347 CATTGC
EcoNI 1924 CCTAGATTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2039 CCTTGGTCAGG
PflFI 2128 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4417 GACCCCGTC
PvuII 276 CAGCTG CAG^CTG
1174 CAGCTG
SbfI 887 CCTGCAGG CCTGCA^GG
909 CCTGCAGG
SmlI 705 CTCAAG C^T[CT][GA]AG
4180 CTCAAG
Sse8387I 887 CCTGCAGG CCTGCA^GG
909 CCTGCAGG
Tth111I 2128 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4417 GACCCCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
Asp700I AspHI AvaII BalI BanI
BanII BbsI BbvCI BbvI BclI
BcnI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I EcoT22I
FauI FbaI Fnu4HI FokI HaeIII
HapII HgaI HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NsiI
NspI PleI PpuMI PstI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SphI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmnI