################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## AatII AccII AccIII AclI AflII AgeI AhdI Aor51HI AscI AseI AsnI AspEI AviII AvrII BamHI BbeI BciVI BfrI BlnI BlpI Bpu1102I BseAI Bsp1407I BspDI BspEI BsrFI BsrGI BssHII Bst1107I BstAPI BstEII BstPI BstUI BstZ17I CelII Cfr10I ClaI CpoI DraIII DrdI Eam1105I Eco47III EcoO65I EcoRI FseI FspI HaeII HindIII HpaI KasI KspI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflMI PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SapI ScaI SexAI SfoI SgrAI SmaI SnaBI SpeI SspBI SwaI Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 700 GGTACC G^GTACC AccI 3149 GTCTAC GT^[AC][GT]AC AcsI 3595 AAATTT [GA]^AATT[CT] AcyI 4404 GGCGTC G[GA]^CG[CT]C AocI 3645 CCTGAGG CC^T[ACGT]AGG ApoI 3595 AAATTT [GA]^AATT[CT] Asp718I 700 GGTACC G^GTACC AvaI 918 CCCGAG C^[CT]CG[GA]G BbrPI 1317 CACGTG CAC^GTG BglII 1938 AGATCT A^GATCT BsaBI 4017 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 4404 GGCGTC G[GA]^CG[CT]C BsaWI 391 ACCGGA [AT]^CCGG[AT] BsiEI 562 CGGCCG CG[GA][CT]^CG BsiWI 1540 CGTACG C^GTACG BsoBI 918 CCCGAG C^[CT]CG[GA]G BspHI 4207 TCATGA T^CATGA BspLU11I 3944 ACATGT A^CATGT BsrBI 12 GAGCGG CCGCTC(-3/-3) BssSI 2084 CTCGTG CACGAG(-5/-1) BstBI 1658 TTCGAA TT^CGAA Bsu36I 3645 CCTGAGG CC^T[ACGT]AGG CfoI 156 GCGC GCG^C DraI 3093 TTTAAA TTT^AAA EagI 562 CGGCCG C^GGCCG EclXI 562 CGGCCG C^GGCCG Eco52I 562 CGGCCG C^GGCCG Eco81I 3645 CCTGAGG CC^T[ACGT]AGG EcoRV 3409 GATATC GAT^ATC HhaI 156 GCGC GCG^C Hin1I 4404 GGCGTC G[GA]^CG[CT]C HinP1I 156 GCGC G^CGC KpnI 700 GGTACC GGTAC^C MamI 4017 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NspV 1658 TTCGAA TT^CGAA PmaCI 1317 CACGTG CAC^GTG PmlI 1317 CACGTG CAC^GTG PshAI 2360 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 4207 TCATGA T^CATGA SfiI 650 GGCCACCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 1658 TTCGAA TT^CGAA ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AflIII 3718 ACGTGT A^C[GA][CT]GT 3944 ACATGT Alw44I 1320 GTGCAC G^TGCAC 1648 GTGCAC ApaI 780 GGGCCC GGGCC^C 915 GGGCCC ApaLI 1320 GTGCAC G^TGCAC 1648 GTGCAC AspI 2128 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4417 GACCCCGTC BcgI 3344 CGACATTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1650 GCACAGTTTTCG BglI 651 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 753 GCCAGTGTGGC BsaAI 1317 CACGTG [CT]AC^GT[GA] 2872 TACGTG BsmBI 4255 CGTCTC CGTCTC(1/5) 4422 CGTCTC BsrDI 2459 CATTGC GCAATG(2/0) 3347 CATTGC EcoNI 1924 CCTAGATTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2039 CCTTGGTCAGG PflFI 2128 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4417 GACCCCGTC PvuII 276 CAGCTG CAG^CTG 1174 CAGCTG SbfI 887 CCTGCAGG CCTGCA^GG 909 CCTGCAGG SmlI 705 CTCAAG C^T[CT][GA]AG 4180 CTCAAG Sse8387I 887 CCTGCAGG CCTGCA^GG 909 CCTGCAGG Tth111I 2128 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4417 GACCCCGTC ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI Asp700I AspHI AvaII BalI BanI BanII BbsI BbvCI BbvI BclI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I EcoT22I FauI FbaI Fnu4HI FokI HaeIII HapII HgaI HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PleI PpuMI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SphI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI