##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AclI AflII AhdI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AviII BbrPI BcgI
BciVI BfrI BglII BsaAI BsgI
BsiWI BspDI BsrBI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI DraII DraIII DrdI EagI
Eam1105I EclXI Eco52I EcoO109I EcoO65I
EcoRV FseI FspI KpnI MfeI
MluI MunI NdeI NheI NotI
NruI NspV PacI PflFI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI SalI SbfI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI StuI SwaI Tth111I
XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccIII 2133 TCCGGA T^CCGGA
AcyI 66 GGCGCC G[GA]^CG[CT]C
AflIII 1383 ACATGT A^C[GA][CT]GT
AgeI 2782 ACCGGT A^CCGGT
Aor51HI 2488 AGCGCT AGC^GCT
AvrII 3195 CCTAGG C^CTAGG
BamHI 3414 GGATCC G^GATCC
BbeI 66 GGCGCC GGCGC^C
BclI 377 TGATCA T^GATCA
BglI 2729 GCCATGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3195 CCTAGG C^CTAGG
BsaBI 1323 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 66 GGCGCC G[GA]^CG[CT]C
BseAI 2133 TCCGGA T^CCGGA
BsmBI 1061 CGTCTC CGTCTC(1/5)
BspEI 2133 TCCGGA T^CCGGA
BspLU11I 1383 ACATGT A^CATGT
CpoI 78 CGGTCCG CG^G[AT]CCG
DraI 503 TTTAAA TTT^AAA
Eco47III 2488 AGCGCT AGC^GCT
EcoT22I 1869 ATGCAT ATGCA^T
FauI 446 GCGGG CCCGC(4/6)
FbaI 377 TGATCA T^GATCA
Hin1I 66 GGCGCC G[GA]^CG[CT]C
HpaI 4039 GTTAAC GTT^AAC
KasI 66 GGCGCC G^GCGCC
KspI 75 CCGCGG CCGC^GG
MamI 1323 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2133 TCCGGA T^CCGGA
NaeI 940 GCCGGC GCC^GGC
NarI 66 GGCGCC GG^CGCC
NgoMIV 940 GCCGGC G^CCGGC
NsiI 1869 ATGCAT ATGCA^T
PaeR7I 2539 CTCGAG C^TCGAG
PinAI 2782 ACCGGT A^CCGGT
PvuII 211 CAGCTG CAG^CTG
RsrII 78 CGGTCCG CG^G[AT]CCG
SacII 75 CCGCGG CCGC^GG
ScaI 1740 AGTACT AGT^ACT
SexAI 2245 ACCAGGT A^CC[AT]GGT
SfoI 66 GGCGCC GGC^GCC
SphI 1045 GCATGC GCATG^C
XhoI 2539 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 645 GTCTAC GT^[AC][GT]AC
3625 GTCTAC
AccII 15 CGCG CG^CG
76 CGCG
Alw44I 862 GTGCAC G^TGCAC
1523 GTGCAC
AocI 2352 CCTCAGG CC^T[ACGT]AGG
2909 CCTGAGG
ApaLI 862 GTGCAC G^TGCAC
1523 GTGCAC
AvaI 867 CCCGAG C^[CT]CG[GA]G
2539 CTCGAG
BanI 66 GGCGCC G^G[CT][GA]CC
887 GGCACC
BcnI 2172 CCGGG CC^[GC]GG
2664 CCCGG
BsaI 2937 GAGACC GGTCTC(1/5)
3176 GAGACC
BsaWI 2133 TCCGGA [AT]^CCGG[AT]
2782 ACCGGT
BsiEI 1916 CGGTCG CG[GA][CT]^CG
2784 CGGTCG
BsmI 663 GAATGC GAATGC(1/-1)
838 GAATGC
BsoBI 867 CCCGAG C^[CT]CG[GA]G
2539 CTCGAG
BsrFI 940 GCCGGC [GA]^CCGG[CT]
2782 ACCGGT
BstUI 15 CGCG CG^CG
76 CGCG
BstXI 246 CCAGAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2246 CCAGGTTTCTGG
Bsu36I 2352 CCTCAGG CC^T[ACGT]AGG
2909 CCTGAGG
Cfr10I 940 GCCGGC [GA]^CCGG[CT]
2782 ACCGGT
Eco81I 2352 CCTCAGG CC^T[ACGT]AGG
2909 CCTGAGG
EcoNI 1884 CCTTTTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3190 CCTCCCCTAGG
HgaI 1445 GCGTC GACGC(5/10)
2450 GCGTC
HincII 4015 GTTGAC GT[CT]^[GA]AC
4039 GTTAAC
HindII 4015 GTTGAC GT[CT]^[GA]AC
4039 GTTAAC
HindIII 1753 AAGCTT A^AGCTT
3457 AAGCTT
MspA1I 75 CCGCGG C[AC]G^C[GT]G
211 CAGCTG
MvnI 15 CGCG CG^CG
76 CGCG
NciI 2172 CCGGG CC^[GC]GG
2664 CCCGG
NcoI 2284 CCATGG C^CATGG
2730 CCATGG
NspI 1045 GCATGC [GA]CATG^[CT]
1383 ACATGT
PflMI 2435 CCATCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3418 CCAAAATGTGG
PstI 134 CTGCAG CTGCA^G
2626 CTGCAG
Van91I 2435 CCATCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3418 CCAAAATGTGG
XbaI 416 TCTAGA T^CTAGA
1628 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BalI BanII BbsI BbvCI BbvI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BspHI BspMI BsrDI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DsaI EaeI EarI EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI RcaI RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI