##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AclI AcyI AflII AgeI
AscI AspI BamHI BbeI BfrI
BsaHI BsiEI BsiWI BspDI BspHI
BssHII BssSI Bst1107I BstAPI BstZ17I
ClaI CpoI DrdI EagI EclXI
Eco52I EcoNI EcoT22I FseI Hin1I
HincII HindII HpaI KasI KspI
MfeI MluI MunI NarI NcoI
NheI NotI NsiI PflFI PinAI
PmeI PpuMI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SexAI
SfiI SfoI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 66 enzymes) #
###################################
Acc65I 1167 GGTACC G^GTACC
AccIII 203 TCCGGA T^CCGGA
AhdI 1181 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 737 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1242 AGCGCT AGC^GCT
ApaI 1197 GGGCCC GGGCC^C
Asp718I 1167 GGTACC G^GTACC
AspEI 1181 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 620 TGCGCA TGC^GCA
AvrII 357 CCTAGG C^CTAGG
BanII 1197 GGGCCC G[GA]GC[CT]^C
BcgI 3411 CGAGCATTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 4270 GCCAACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 357 CCTAGG C^CTAGG
BlpI 1385 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1385 GCTTAGC GC^T[ACGT]AGC
BsaBI 1281 GATATCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2113 GAGACC GGTCTC(1/5)
BseAI 203 TCCGGA T^CCGGA
BsmBI 2108 GAGACG CGTCTC(1/5)
BspEI 203 TCCGGA T^CCGGA
BspLU11I 3920 ACATGT A^CATGT
BsrDI 1436 CATTGC GCAATG(2/0)
BsrFI 527 GCCGGC [GA]^CCGG[CT]
BstBI 760 TTCGAA TT^CGAA
BstEII 1296 GGTCACC G^GT[ACGT]ACC
BstPI 1296 GGTCACC G^GT[ACGT]ACC
CelII 1385 GCTTAGC GC^T[ACGT]AGC
Cfr10I 527 GCCGGC [GA]^CCGG[CT]
DraI 1257 TTTAAA TTT^AAA
DraII 1197 GGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 2118 CCACGG C^C[GA][CT]GG
Eam1105I 1181 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1242 AGCGCT AGC^GCT
EcoO109I 1197 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1296 GGTCACC G^GT[ACGT]ACC
EcoRI 3127 GAATTC G^AATTC
FauI 512 GCGGG CCCGC(4/6)
FspI 620 TGCGCA TGC^GCA
HaeII 1242 AGCGCT [GA]GCGC^[CT]
KpnI 1167 GGTACC GGTAC^C
MamI 1281 GATATCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 203 TCCGGA T^CCGGA
NaeI 527 GCCGGC GCC^GGC
NdeI 2414 CATATG CA^TATG
NgoMIV 527 GCCGGC G^CCGGC
NruI 80 TCGCGA TCG^CGA
NspV 760 TTCGAA TT^CGAA
PacI 3439 TTAATTAA TTAAT^TAA
PaeR7I 1565 CTCGAG C^TCGAG
PshAI 696 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1610 CAGCTG CAG^CTG
SapI 1928 GCTCTTC GCTCTTC(1/4)
SbfI 326 CCTGCAGG CCTGCA^GG
SfuI 760 TTCGAA TT^CGAA
SgrAI 526 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 2162 CCCGGG CCC^GGG
SnaBI 2035 TACGTA TAC^GTA
SpeI 2855 ACTAGT A^CTAGT
SphI 321 GCATGC GCATG^C
Sse8387I 326 CCTGCAGG CCTGCA^GG
XbaI 3049 TCTAGA T^CTAGA
XcmI 1175 CCATGCGACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1565 CTCGAG C^TCGAG
XmaCI 2162 CCCGGG C^CCGGG
XmaI 2162 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 2400 GTCTAC GT^[AC][GT]AC
3284 GTCTAC
AflIII 3311 ACGTGT A^C[GA][CT]GT
3920 ACATGT
Alw44I 3339 GTGCAC G^TGCAC
4328 GTGCAC
AocI 1537 CCTAAGG CC^T[ACGT]AGG
4241 CCTGAGG
ApaLI 3339 GTGCAC G^TGCAC
4328 GTGCAC
BalI 749 TGGCCA TGG^CCA
4268 TGGCCA
BanI 1167 GGTACC G^G[CT][GA]CC
2153 GGCACC
BbrPI 2068 CACGTG CAC^GTG
3310 CACGTG
BciVI 1461 GGATAC GTATCC(6/5)
2647 GTATCC
BclI 1836 TGATCA T^GATCA
2948 TGATCA
BglII 261 AGATCT A^GATCT
4143 AGATCT
BsaWI 203 TCCGGA [AT]^CCGG[AT]
2542 TCCGGT
BspMI 4334 ACCTGC ACCTGC(4/8)
3792 GCAGGT
Bsu36I 1537 CCTAAGG CC^T[ACGT]AGG
4241 CCTGAGG
DraIII 2087 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
3342 CACCGAGTG
EaeI 749 TGGCCA [CT]^GGCC[GA]
4268 TGGCCA
Eco81I 1537 CCTAAGG CC^T[ACGT]AGG
4241 CCTGAGG
FbaI 1836 TGATCA T^GATCA
2948 TGATCA
HgaI 1456 GACGC GACGC(5/10)
143 GCGTC
MluNI 749 TGGCCA TGG^CCA
4268 TGGCCA
MscI 749 TGGCCA TGG^CCA
4268 TGGCCA
MspA1I 1301 CCGCTG C[AC]G^C[GT]G
1610 CAGCTG
PflMI 736 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4384 CCAGGAGGTGG
PmaCI 2068 CACGTG CAC^GTG
3310 CACGTG
PmlI 2068 CACGTG CAC^GTG
3310 CACGTG
ScaI 68 AGTACT AGT^ACT
2858 AGTACT
SspI 2433 AATATT AAT^ATT
3902 AATATT
StuI 44 AGGCCT AGG^CCT
1709 AGGCCT
Van91I 736 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4384 CCAGGAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI Asp700I
AspHI AvaI AvaII BbsI BbvCI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BsrBI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EarI EcoRII
EcoRV EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PshBI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI