################################## # NO CUT ENZYMES ( 59 enzymes) # ################################## AatII AclI AcyI AflII AgeI AscI AspI BamHI BbeI BfrI BsaHI BsiEI BsiWI BspDI BspHI BssHII BssSI Bst1107I BstAPI BstZ17I ClaI CpoI DrdI EagI EclXI Eco52I EcoNI EcoT22I FseI Hin1I HincII HindII HpaI KasI KspI MfeI MluI MunI NarI NcoI NheI NotI NsiI PflFI PinAI PmeI PpuMI Psp1406I PvuI RcaI RsrII SacI SacII SalI SexAI SfiI SfoI SwaI Tth111I ################################### # ONE CUT ENZYMES ( 66 enzymes) # ################################### Acc65I 1167 GGTACC G^GTACC AccIII 203 TCCGGA T^CCGGA AhdI 1181 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 737 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 1242 AGCGCT AGC^GCT ApaI 1197 GGGCCC GGGCC^C Asp718I 1167 GGTACC G^GTACC AspEI 1181 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 620 TGCGCA TGC^GCA AvrII 357 CCTAGG C^CTAGG BanII 1197 GGGCCC G[GA]GC[CT]^C BcgI 3411 CGAGCATTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 4270 GCCAACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 357 CCTAGG C^CTAGG BlpI 1385 GCTTAGC GC^T[ACGT]AGC Bpu1102I 1385 GCTTAGC GC^T[ACGT]AGC BsaBI 1281 GATATCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 2113 GAGACC GGTCTC(1/5) BseAI 203 TCCGGA T^CCGGA BsmBI 2108 GAGACG CGTCTC(1/5) BspEI 203 TCCGGA T^CCGGA BspLU11I 3920 ACATGT A^CATGT BsrDI 1436 CATTGC GCAATG(2/0) BsrFI 527 GCCGGC [GA]^CCGG[CT] BstBI 760 TTCGAA TT^CGAA BstEII 1296 GGTCACC G^GT[ACGT]ACC BstPI 1296 GGTCACC G^GT[ACGT]ACC CelII 1385 GCTTAGC GC^T[ACGT]AGC Cfr10I 527 GCCGGC [GA]^CCGG[CT] DraI 1257 TTTAAA TTT^AAA DraII 1197 GGGCCCT [GA]G^G[ACGT]CC[CT] DsaI 2118 CCACGG C^C[GA][CT]GG Eam1105I 1181 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1242 AGCGCT AGC^GCT EcoO109I 1197 GGGCCCT [GA]G^G[ACGT]CC[CT] EcoO65I 1296 GGTCACC G^GT[ACGT]ACC EcoRI 3127 GAATTC G^AATTC FauI 512 GCGGG CCCGC(4/6) FspI 620 TGCGCA TGC^GCA HaeII 1242 AGCGCT [GA]GCGC^[CT] KpnI 1167 GGTACC GGTAC^C MamI 1281 GATATCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 203 TCCGGA T^CCGGA NaeI 527 GCCGGC GCC^GGC NdeI 2414 CATATG CA^TATG NgoMIV 527 GCCGGC G^CCGGC NruI 80 TCGCGA TCG^CGA NspV 760 TTCGAA TT^CGAA PacI 3439 TTAATTAA TTAAT^TAA PaeR7I 1565 CTCGAG C^TCGAG PshAI 696 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 1610 CAGCTG CAG^CTG SapI 1928 GCTCTTC GCTCTTC(1/4) SbfI 326 CCTGCAGG CCTGCA^GG SfuI 760 TTCGAA TT^CGAA SgrAI 526 CGCCGGCG C[GA]^CCGG[CT]G SmaI 2162 CCCGGG CCC^GGG SnaBI 2035 TACGTA TAC^GTA SpeI 2855 ACTAGT A^CTAGT SphI 321 GCATGC GCATG^C Sse8387I 326 CCTGCAGG CCTGCA^GG XbaI 3049 TCTAGA T^CTAGA XcmI 1175 CCATGCGACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1565 CTCGAG C^TCGAG XmaCI 2162 CCCGGG C^CCGGG XmaI 2162 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccI 2400 GTCTAC GT^[AC][GT]AC 3284 GTCTAC AflIII 3311 ACGTGT A^C[GA][CT]GT 3920 ACATGT Alw44I 3339 GTGCAC G^TGCAC 4328 GTGCAC AocI 1537 CCTAAGG CC^T[ACGT]AGG 4241 CCTGAGG ApaLI 3339 GTGCAC G^TGCAC 4328 GTGCAC BalI 749 TGGCCA TGG^CCA 4268 TGGCCA BanI 1167 GGTACC G^G[CT][GA]CC 2153 GGCACC BbrPI 2068 CACGTG CAC^GTG 3310 CACGTG BciVI 1461 GGATAC GTATCC(6/5) 2647 GTATCC BclI 1836 TGATCA T^GATCA 2948 TGATCA BglII 261 AGATCT A^GATCT 4143 AGATCT BsaWI 203 TCCGGA [AT]^CCGG[AT] 2542 TCCGGT BspMI 4334 ACCTGC ACCTGC(4/8) 3792 GCAGGT Bsu36I 1537 CCTAAGG CC^T[ACGT]AGG 4241 CCTGAGG DraIII 2087 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG 3342 CACCGAGTG EaeI 749 TGGCCA [CT]^GGCC[GA] 4268 TGGCCA Eco81I 1537 CCTAAGG CC^T[ACGT]AGG 4241 CCTGAGG FbaI 1836 TGATCA T^GATCA 2948 TGATCA HgaI 1456 GACGC GACGC(5/10) 143 GCGTC MluNI 749 TGGCCA TGG^CCA 4268 TGGCCA MscI 749 TGGCCA TGG^CCA 4268 TGGCCA MspA1I 1301 CCGCTG C[AC]G^C[GT]G 1610 CAGCTG PflMI 736 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4384 CCAGGAGGTGG PmaCI 2068 CACGTG CAC^GTG 3310 CACGTG PmlI 2068 CACGTG CAC^GTG 3310 CACGTG ScaI 68 AGTACT AGT^ACT 2858 AGTACT SspI 2433 AATATT AAT^ATT 3902 AATATT StuI 44 AGGCCT AGG^CCT 1709 AGGCCT Van91I 736 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4384 CCAGGAGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 104 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI AseI AsnI Asp700I AspHI AvaI AvaII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BsrBI BsrGI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII EcoRV EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PshBI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI