##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AcyI AflII AgeI
AscI AseI AsnI Asp718I AspI
AvrII BbeI BbrPI BbvCI BcgI
BfrI BlnI BlpI Bpu1102I BsaHI
BsiWI BsmI Bsp1407I BsrGI Bst1107I
BstBI BstZ17I CelII CpoI DraI
EcoRV FseI Hin1I HpaI KasI
KpnI KspI MfeI MluI MunI
NarI NdeI NotI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshBI PvuI RsrII SacII SalI
SbfI ScaI SexAI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 697 GTCTAC GT^[AC][GT]AC
AccIII 1325 TCCGGA T^CCGGA
AclI 1165 AACGTT AA^CGTT
AhdI 1226 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2518 GTGCAC G^TGCAC
ApaLI 2518 GTGCAC G^TGCAC
Asp700I 2554 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1226 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2852 TGCGCA TGC^GCA
BamHI 3569 GGATCC G^GATCC
BciVI 2664 GTATCC GTATCC(6/5)
BclI 1515 TGATCA T^GATCA
BglI 12 GCCGCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 2209 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1325 TCCGGA T^CCGGA
BsmBI 556 CGTCTC CGTCTC(1/5)
BspDI 2266 ATCGAT AT^CGAT
BspEI 1325 TCCGGA T^CCGGA
BspHI 2487 TCATGA T^CATGA
BssHII 68 GCGCGC G^CGCGC
BstAPI 2855 GCACTGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 2266 ATCGAT AT^CGAT
DraIII 2856 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3276 GACCTCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1226 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 757 GAATTC G^AATTC
EcoT22I 3950 ATGCAT ATGCA^T
FbaI 1515 TGATCA T^GATCA
FspI 2852 TGCGCA TGC^GCA
HgaI 555 GCGTC GACGC(5/10)
HindIII 800 AAGCTT A^AGCTT
MamI 2209 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1325 TCCGGA T^CCGGA
NaeI 989 GCCGGC GCC^GGC
NgoMIV 989 GCCGGC G^CCGGC
NheI 2422 GCTAGC G^CTAGC
NruI 594 TCGCGA TCG^CGA
NsiI 3950 ATGCAT ATGCA^T
PshAI 854 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1165 AACGTT AA^CGTT
RcaI 2487 TCATGA T^CATGA
SacI 1729 GAGCTC GAGCT^C
SfiI 11 GGCCGCTCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1350 GCATGC GCATG^C
XmnI 2554 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 757 GAATTC [GA]^AATT[CT]
2779 GAATTT
ApoI 757 GAATTC [GA]^AATT[CT]
2779 GAATTT
AvaI 124 CTCGAG C^[CT]CG[GA]G
2993 CTCGAG
BcnI 8 CCCGG CC^[GC]GG
3861 CCGGG
BglII 275 AGATCT A^GATCT
2324 AGATCT
BsaAI 169 TACGTG [CT]AC^GT[GA]
721 CACGTA
BsaWI 214 ACCGGA [AT]^CCGG[AT]
1325 TCCGGA
BsgI 1885 CTGCAC GTGCAG(16/14)
2284 CTGCAC
BsoBI 124 CTCGAG C^[CT]CG[GA]G
2993 CTCGAG
BspLU11I 155 ACATGT A^CATGT
2189 ACATGT
BspMI 380 ACCTGC ACCTGC(4/8)
968 ACCTGC
BsrFI 508 ACCGGC [GA]^CCGG[CT]
989 GCCGGC
BstEII 285 GGTCACC G^GT[ACGT]ACC
3273 GGTGACC
BstPI 285 GGTCACC G^GT[ACGT]ACC
3273 GGTGACC
BstXI 1604 CCAAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3132 CCAGGAGGTTGG
Cfr10I 508 ACCGGC [GA]^CCGG[CT]
989 GCCGGC
EagI 10 CGGCCG C^GGCCG
230 CGGCCG
EclXI 10 CGGCCG C^GGCCG
230 CGGCCG
Eco52I 10 CGGCCG C^GGCCG
230 CGGCCG
EcoO65I 285 GGTCACC G^GT[ACGT]ACC
3273 GGTGACC
FauI 1 CCCGC CCCGC(4/6)
3729 GCGGG
NciI 8 CCCGG CC^[GC]GG
3861 CCGGG
PaeR7I 124 CTCGAG C^TCGAG
2993 CTCGAG
PvuII 1276 CAGCTG CAG^CTG
3688 CAGCTG
SapI 993 GCTCTTC GCTCTTC(1/4)
3240 GCTCTTC
XhoI 124 CTCGAG C^TCGAG
2993 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AocI Aor51HI ApaI
AspHI AvaII BalI BanI BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrBI BsrDI BsrI BssKI
BssSI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco47III Eco81I EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII