##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AccIII AcsI AflII AflIII AgeI
Aor51HI ApoI AscI AseI AsnI
AspI AviII AvrII BcgI BclI
BfrI BlnI BseAI BsiWI BspDI
BspEI BspLU11I BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraI DraIII
Eco47III EcoRI EcoT22I FbaI FseI
FspI HpaI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshBI PvuI RsrII
SalI SbfI SexAI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 1037 GACGTC GACGT^C
Acc65I 2744 GGTACC G^GTACC
AccI 407 GTCTAC GT^[AC][GT]AC
AclI 2555 AACGTT AA^CGTT
AhdI 2860 GACAACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 2744 GGTACC G^GTACC
AspEI 2860 GACAACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 267 GGATCC G^GATCC
BbeI 933 GGCGCC GGCGC^C
BbrPI 2071 CACGTG CAC^GTG
BglII 1290 AGATCT A^GATCT
BsaAI 2071 CACGTG [CT]AC^GT[GA]
BsaWI 3218 ACCGGA [AT]^CCGG[AT]
BsmBI 1610 GAGACG CGTCTC(1/5)
BsmI 2771 GAATGC GAATGC(1/-1)
Bsp1407I 360 TGTACA T^GTACA
BsrGI 360 TGTACA T^GTACA
BstAPI 253 GCACAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EagI 78 CGGCCG C^GGCCG
Eam1105I 2860 GACAACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 78 CGGCCG C^GGCCG
Eco52I 78 CGGCCG C^GGCCG
HincII 2508 GTCAAC GT[CT]^[GA]AC
HindII 2508 GTCAAC GT[CT]^[GA]AC
HindIII 3586 AAGCTT A^AGCTT
KasI 933 GGCGCC G^GCGCC
KpnI 2744 GGTACC GGTAC^C
KspI 1552 CCGCGG CCGC^GG
NarI 933 GGCGCC GG^CGCC
PmaCI 2071 CACGTG CAC^GTG
PmlI 2071 CACGTG CAC^GTG
PshAI 2656 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2555 AACGTT AA^CGTT
SacII 1552 CCGCGG CCGC^GG
SapI 286 GCTCTTC GCTCTTC(1/4)
SfoI 933 GGCGCC GGC^GCC
SspBI 360 TGTACA T^GTACA
StuI 846 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Alw44I 215 GTGCAC G^TGCAC
3078 GTGCAC
ApaI 816 GGGCCC GGGCC^C
1909 GGGCCC
ApaLI 215 GTGCAC G^TGCAC
3078 GTGCAC
BbvCI 943 CCTCAGC CCTCAGC(-5/-2)
3066 CCTCAGC
BciVI 3997 GGATAC GTATCC(6/5)
2118 GTATCC
BlpI 10 GCTGAGC GC^T[ACGT]AGC
4025 GCTGAGC
Bpu1102I 10 GCTGAGC GC^T[ACGT]AGC
4025 GCTGAGC
BsiEI 78 CGGCCG CG[GA][CT]^CG
2286 CGACCG
BspHI 1677 TCATGA T^CATGA
2802 TCATGA
BssSI 593 CTCGTG CACGAG(-5/-1)
3403 CTCGTG
BstEII 334 GGTGACC G^GT[ACGT]ACC
3702 GGTGACC
BstPI 334 GGTGACC G^GT[ACGT]ACC
3702 GGTGACC
CelII 10 GCTGAGC GC^T[ACGT]AGC
4025 GCTGAGC
DrdI 95 GACCAGGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3088 GACCAGACTGTC
EcoO65I 334 GGTGACC G^GT[ACGT]ACC
3702 GGTGACC
EcoRV 2297 GATATC GAT^ATC
2591 GATATC
HaeII 933 GGCGCC [GA]GCGC^[CT]
3028 AGCGCC
NaeI 2621 GCCGGC GCC^GGC
2932 GCCGGC
NgoMIV 2621 GCCGGC G^CCGGC
2932 GCCGGC
NspI 363 ACATGC [GA]CATG^[CT]
3679 GCATGT
RcaI 1677 TCATGA T^CATGA
2802 TCATGA
ScaI 1464 AGTACT AGT^ACT
3449 AGTACT
SfiI 1985 GGCCTCACCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3745 GGCCTGGTGGGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaBI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI XmnI