##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII AccIII AclI AflII AhdI
Aor51HI AscI AseI AsnI AspEI
AvrII BamHI BfrI BlnI BseAI
BsiWI BsmI BspDI BspEI BspLU11I
BsrDI BstAPI BstBI BstEII BstPI
ClaI CpoI DraI DrdI Eam1105I
Eco47III EcoO65I EcoRI MfeI MluI
MroI MunI NheI NotI NruI
NspV PacI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI ScaI
SfuI SnaBI SpeI SspI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AcsI 3160 GAATTT [GA]^AATT[CT]
AgeI 2832 ACCGGT A^CCGGT
ApoI 3160 GAATTT [GA]^AATT[CT]
Asp700I 2420 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI 2090 GCTGAGG CCTCAGC(-5/-2)
BcgI 749 CGAGGACGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3150 GTATCC GTATCC(6/5)
BclI 1999 TGATCA T^GATCA
BglII 835 AGATCT A^GATCT
BsaBI 2567 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1382 CTGCAC GTGCAG(16/14)
Bsp1407I 3218 TGTACA T^GTACA
BspHI 1135 TCATGA T^CATGA
BsrGI 3218 TGTACA T^GTACA
Bst1107I 768 GTATAC GTA^TAC
BstZ17I 768 GTATAC GTA^TAC
EcoRV 2145 GATATC GAT^ATC
EcoT22I 3019 ATGCAT ATGCA^T
FbaI 1999 TGATCA T^GATCA
FseI 3852 GGCCGGCC GGCCGG^CC
HindIII 1952 AAGCTT A^AGCTT
HpaI 2640 GTTAAC GTT^AAC
MamI 2567 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 2641 TTAA T^TAA
NcoI 2786 CCATGG C^CATGG
NdeI 2190 CATATG CA^TATG
NsiI 3019 ATGCAT ATGCA^T
PinAI 2832 ACCGGT A^CCGGT
RcaI 1135 TCATGA T^CATGA
SgrAI 2831 CACCGGTG C[GA]^CCGG[CT]G
SspBI 3218 TGTACA T^GTACA
Tru9I 2641 TTAA T^TAA
XmnI 2420 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 1249 GGTACC G^GTACC
2945 GGTACC
AlwI 1283 GATCC GGATC(4/5)
2557 GGATC
Asp718I 1249 GGTACC G^GTACC
2945 GGTACC
AspI 1362 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1880 GACCCTGTC
AviII 490 TGCGCA TGC^GCA
2362 TGCGCA
BbrPI 1788 CACGTG CAC^GTG
3005 CACGTG
BsaI 3063 GAGACC GGTCTC(1/5)
3759 GAGACC
BstXI 289 CCATCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2414 CCACGAGAATGG
BstYI 835 AGATCT [GA]^GATC[CT]
2557 GGATCT
EagI 1835 CGGCCG C^GGCCG
2052 CGGCCG
EclXI 1835 CGGCCG C^GGCCG
2052 CGGCCG
Eco52I 1835 CGGCCG C^GGCCG
2052 CGGCCG
EcoNI 962 CCTCGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3193 CCTGGGACAGG
FspI 490 TGCGCA TGC^GCA
2362 TGCGCA
KpnI 1249 GGTACC GGTAC^C
2945 GGTACC
MflI 835 AGATCT [GA]^GATC[CT]
2557 GGATCT
PaeR7I 963 CTCGAG C^TCGAG
1243 CTCGAG
PflFI 1362 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1880 GACCCTGTC
PflMI 520 CCAGCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1012 CCAACATCTGG
PmaCI 1788 CACGTG CAC^GTG
3005 CACGTG
PmlI 1788 CACGTG CAC^GTG
3005 CACGTG
SacI 957 GAGCTC GAGCT^C
2135 GAGCTC
SapI 3704 GAAGAGC GCTCTTC(1/4)
1034 GCTCTTC
SbfI 2660 CCTGCAGG CCTGCA^GG
2847 CCTGCAGG
SexAI 1507 ACCTGGT A^CC[AT]GGT
2311 ACCTGGT
SfiI 2225 GGCCATCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3843 GGCCCAAGTGGCC
SphI 2255 GCATGC GCATG^C
2359 GCATGC
Sse8387I 2660 CCTGCAGG CCTGCA^GG
2847 CCTGCAGG
Tth111I 1362 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1880 GACCCTGTC
Van91I 520 CCAGCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1012 CCAACATCTGG
XhoI 963 CTCGAG C^TCGAG
1243 CTCGAG
XhoII 835 AGATCT [GA]^GATC[CT]
2557 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI Alw44I AlwNI AocI
ApaI ApaLI AspHI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaJI BsaWI BseRI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssHII BssKI BssSI
BstF5I BstNI BstUI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
StuI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XmaCI XmaI