##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BfrI BlnI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspHI BsrDI BsrGI
Bst1107I BstBI BstZ17I ClaI CpoI
DraIII EagI Eam1105I EclXI Eco52I
EcoRI EcoT22I FseI FspI HincII
HindII HindIII HpaI KpnI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SfiI SfuI SmlI SnaBI
SpeI Sse8387I SspBI SwaI TaqI
Tth111I TthHB8I XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
Aor51HI 301 AGCGCT AGC^GCT
ApaI 1323 GGGCCC GGGCC^C
Asp700I 480 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 2850 GAAGAC GAAGAC(2/6)
BclI 4059 TGATCA T^GATCA
BglII 2321 AGATCT A^GATCT
BpuAI 2850 GAAGAC GAAGAC(2/6)
BsaBI 2446 GATTCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 1874 GAATGC GAATGC(1/-1)
BspLU11I 1784 ACATGT A^CATGT
BsrBI 1293 GAGCGG CCGCTC(-3/-3)
BsrFI 278 GCCGGC [GA]^CCGG[CT]
BssHII 176 GCGCGC G^CGCGC
BstEII 2278 GGTCACC G^GT[ACGT]ACC
BstPI 2278 GGTCACC G^GT[ACGT]ACC
Cfr10I 278 GCCGGC [GA]^CCGG[CT]
DrdI 2534 GACTGTCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 301 AGCGCT AGC^GCT
EcoO65I 2278 GGTCACC G^GT[ACGT]ACC
EcoRV 1253 GATATC GAT^ATC
FbaI 4059 TGATCA T^GATCA
HgaI 1404 GACGC GACGC(5/10)
KspI 183 CCGCGG CCGC^GG
MamI 2446 GATTCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 278 GCCGGC GCC^GGC
NgoMIV 278 GCCGGC G^CCGGC
PpuMI 2898 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 1386 CAGCTG CAG^CTG
SacII 183 CCGCGG CCGC^GG
SexAI 773 ACCTGGT A^CC[AT]GGT
SgrAI 277 CGCCGGCG C[GA]^CCGG[CT]G
XcmI 105 CCATCCCAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 480 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccIII 2575 TCCGGA T^CCGGA
2935 TCCGGA
BamHI 736 GGATCC G^GATCC
1056 GGATCC
BbeI 275 GGCGCC GGCGC^C
349 GGCGCC
BcgI 3323 CGATTATCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
374 GCACTGCCCTCG
BciVI 1117 GTATCC GTATCC(6/5)
1788 GTATCC
BsaI 441 GGTCTC GGTCTC(1/5)
4140 GGTCTC
BseAI 2575 TCCGGA T^CCGGA
2935 TCCGGA
BsgI 2612 CTGCAC GTGCAG(16/14)
3907 CTGCAC
BspEI 2575 TCCGGA T^CCGGA
2935 TCCGGA
BstAPI 205 GCAGCGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3298 GCAACCTCTGC
KasI 275 GGCGCC G^GCGCC
349 GGCGCC
MroI 2575 TCCGGA T^CCGGA
2935 TCCGGA
MspA1I 183 CCGCGG C[AC]G^C[GT]G
1386 CAGCTG
NarI 275 GGCGCC GG^CGCC
349 GGCGCC
NcoI 732 CCATGG C^CATGG
1841 CCATGG
PshAI 1765 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3238 GACAAGAGTC
SacI 1025 GAGCTC GAGCT^C
3694 GAGCTC
SapI 2683 GCTCTTC GCTCTTC(1/4)
3696 GCTCTTC
SfoI 275 GGCGCC GGC^GCC
349 GGCGCC
SphI 2870 GCATGC GCATG^C
3364 GCATGC
SspI 1738 AATATT AAT^ATT
1812 AATATT
StuI 1042 AGGCCT AGG^CCT
4011 AGGCCT
XbaI 3053 TCTAGA T^CTAGA
3637 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaLI ApoI AspHI AvaI
AvaII BalI BanI BanII BbrPI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaHI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XhoII XmaCI XmaI