##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
Aor51HI AscI Asp700I AspEI AviII
BamHI BbeI BbrPI BfrI BsaWI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BsrBI BssHII BstAPI BstBI
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRV
EcoT22I FseI FspI HpaI KasI
MluI MroI NarI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacI SalI
SapI SbfI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I StuI SwaI
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 39 GACGTC GACGT^C
Acc65I 2197 GGTACC G^GTACC
AocI 55 CCTCAGG CC^T[ACGT]AGG
Asp718I 2197 GGTACC G^GTACC
AspI 1560 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2019 CCTAGG C^CTAGG
BcgI 880 GCAGCAAAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 456 TGATCA T^GATCA
BglII 4135 AGATCT A^GATCT
BlnI 2019 CCTAGG C^CTAGG
BsaAI 224 TACGTG [CT]AC^GT[GA]
BsaBI 3490 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 3716 CTCCTC GAGGAG(10/8)
Bsp1407I 3492 TGTACA T^GTACA
BspHI 564 TCATGA T^CATGA
BsrFI 1951 GCCGGC [GA]^CCGG[CT]
BsrGI 3492 TGTACA T^GTACA
BssSI 183 CTCGTG CACGAG(-5/-1)
Bst1107I 3479 GTATAC GTA^TAC
BstEII 1650 GGTGACC G^GT[ACGT]ACC
BstPI 1650 GGTGACC G^GT[ACGT]ACC
BstZ17I 3479 GTATAC GTA^TAC
Bsu36I 55 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1951 GCCGGC [GA]^CCGG[CT]
DrdI 2635 GACATGCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 3270 GAAGAG CTCTTC(1/4)
Eco81I 55 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1650 GGTGACC G^GT[ACGT]ACC
FbaI 456 TGATCA T^GATCA
KpnI 2197 GGTACC GGTAC^C
Ksp632I 3270 GAAGAG CTCTTC(1/4)
KspI 8 CCGCGG CCGC^GG
MamI 3490 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1951 GCCGGC GCC^GGC
NdeI 3095 CATATG CA^TATG
NgoMIV 1951 GCCGGC G^CCGGC
PflFI 1560 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2938 CCAGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 208 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 564 TCATGA T^CATGA
SacII 8 CCGCGG CCGC^GG
ScaI 4169 AGTACT AGT^ACT
SexAI 680 ACCAGGT A^CC[AT]GGT
SfiI 1287 GGCCACTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 22 CCCGGG CCC^GGG
SphI 3224 GCATGC GCATG^C
SspBI 3492 TGTACA T^GTACA
Tth111I 1560 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2938 CCAGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 22 CCCGGG C^CCGGG
XmaI 22 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 39 GACGTC G[GA]^CG[CT]C
2395 GGCGTC
Alw44I 2732 GTGCAC G^TGCAC
4182 GTGCAC
ApaLI 2732 GTGCAC G^TGCAC
4182 GTGCAC
AseI 3914 ATTAAT AT^TAAT
4336 ATTAAT
AsnI 3914 ATTAAT AT^TAAT
4336 ATTAAT
AspHI 2732 GTGCAC G[AT]GC[AT]^C
4182 GTGCAC
AvaI 22 CCCGGG C^[CT]CG[GA]G
2025 CTCGGG
BalI 731 TGGCCA TGG^CCA
1286 TGGCCA
BbvCI 1189 GCTGAGG CCTCAGC(-5/-2)
3079 GCTGAGG
BciVI 1601 GGATAC GTATCC(6/5)
1797 GTATCC
BsaHI 39 GACGTC G[GA]^CG[CT]C
2395 GGCGTC
BsgI 2687 GTGCAG GTGCAG(16/14)
2954 GTGCAG
BsiHKAI 2732 GTGCAC G[AT]GC[AT]^C
4182 GTGCAC
BsoBI 22 CCCGGG C^[CT]CG[GA]G
2025 CTCGGG
BspLU11I 1895 ACATGT A^CATGT
3361 ACATGT
HaeII 4 AGCGCC [GA]GCGC^[CT]
282 AGCGCC
Hin1I 39 GACGTC G[GA]^CG[CT]C
2395 GGCGTC
HincII 1566 GTCAAC GT[CT]^[GA]AC
1852 GTCAAC
HindII 1566 GTCAAC GT[CT]^[GA]AC
1852 GTCAAC
HindIII 437 AAGCTT A^AGCTT
2544 AAGCTT
MluNI 731 TGGCCA TGG^CCA
1286 TGGCCA
MscI 731 TGGCCA TGG^CCA
1286 TGGCCA
NcoI 728 CCATGG C^CATGG
2852 CCATGG
PshBI 3914 ATTAAT AT^TAAT
4336 ATTAAT
XcmI 1655 CCACTTTTGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2975 CCATTGCTGATTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI AvaII BanI BanII BbsI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MfeI MflI MnlI
MseI MslI MspA1I MspI MunI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII