##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
ApaI AscI Asp718I AspEI AviII
AvrII BamHI BbeI BbvCI BglI
BlnI BlpI Bpu1102I CelII CpoI
DraII DrdI EagI Eam1105I EclXI
Eco52I EcoO109I EcoRI EcoT22I FseI
FspI KasI KpnI KspI MfeI
MluI MunI NarI NotI NruI
NsiI PinAI PmeI PpuMI PshAI
Psp1406I RsrII SacII SalI SbfI
SexAI SfiI SfoI SnaBI SphI
Sse8387I StuI SwaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Alw44I 3140 GTGCAC G^TGCAC
AocI 6273 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2813 AGCGCT AGC^GCT
ApaLI 3140 GTGCAC G^TGCAC
AspI 250 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 3908 GCAGCAGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsiEI 2541 CGATCG CG[GA][CT]^CG
BsiWI 1960 CGTACG C^GTACG
Bsp1407I 5779 TGTACA T^GTACA
BspDI 4250 ATCGAT AT^CGAT
BsrDI 3479 GCAATG GCAATG(2/0)
BsrGI 5779 TGTACA T^GTACA
Bst1107I 6381 GTATAC GTA^TAC
BstBI 5150 TTCGAA TT^CGAA
BstEII 569 GGTCACC G^GT[ACGT]ACC
BstPI 569 GGTCACC G^GT[ACGT]ACC
BstXI 3984 CCATCGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 6381 GTATAC GTA^TAC
Bsu36I 6273 CCTGAGG CC^T[ACGT]AGG
ClaI 4250 ATCGAT AT^CGAT
Eco47III 2813 AGCGCT AGC^GCT
Eco81I 6273 CCTGAGG CC^T[ACGT]AGG
EcoO65I 569 GGTCACC G^GT[ACGT]ACC
HincII 6254 GTTAAC GT[CT]^[GA]AC
HindII 6254 GTTAAC GT[CT]^[GA]AC
HindIII 5192 AAGCTT A^AGCTT
HpaI 6254 GTTAAC GTT^AAC
NaeI 2861 GCCGGC GCC^GGC
NcoI 5681 CCATGG C^CATGG
NdeI 6636 CATATG CA^TATG
NgoMIV 2861 GCCGGC G^CCGGC
NspV 5150 TTCGAA TT^CGAA
PacI 5911 TTAATTAA TTAAT^TAA
PaeR7I 1697 CTCGAG C^TCGAG
PflFI 250 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 1055 CTGCAG CTGCA^G
PvuI 2541 CGATCG CGAT^CG
SfuI 5150 TTCGAA TT^CGAA
SgrAI 2860 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 5502 ACTAGT A^CTAGT
SspBI 5779 TGTACA T^GTACA
Tth111I 250 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 4200 CCATTCAAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1697 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 298 TCCGGA T^CCGGA
2877 TCCGGA
AcyI 1577 GACGCC G[GA]^CG[CT]C
3849 GACGCC
BanI 292 GGTGCC G^G[CT][GA]CC
5179 GGCACC
BbrPI 3856 CACGTG CAC^GTG
5788 CACGTG
BclI 1545 TGATCA T^GATCA
4095 TGATCA
BglII 4207 AGATCT A^GATCT
4599 AGATCT
BsaHI 1577 GACGCC G[GA]^CG[CT]C
3849 GACGCC
BsaWI 298 TCCGGA [AT]^CCGG[AT]
2877 TCCGGA
BseAI 298 TCCGGA T^CCGGA
2877 TCCGGA
BsmBI 1481 CGTCTC CGTCTC(1/5)
3847 GAGACG
BspEI 298 TCCGGA T^CCGGA
2877 TCCGGA
BsrFI 182 GCCGGT [GA]^CCGG[CT]
2861 GCCGGC
BssHII 199 GCGCGC G^CGCGC
201 GCGCGC
BstAPI 3691 GCAACTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4762 GCAGGTGATGC
Cfr10I 182 GCCGGT [GA]^CCGG[CT]
2861 GCCGGC
DraIII 533 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
5759 CACATTGTG
DsaI 3433 CCACGG C^C[GA][CT]GG
5681 CCATGG
EcoNI 6530 CCTCTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
6667 CCTGCCAAAGG
EcoRV 1159 GATATC GAT^ATC
2162 GATATC
FbaI 1545 TGATCA T^GATCA
4095 TGATCA
Hin1I 1577 GACGCC G[GA]^CG[CT]C
3849 GACGCC
MroI 298 TCCGGA T^CCGGA
2877 TCCGGA
NheI 171 GCTAGC G^CTAGC
1030 GCTAGC
PflMI 621 CCAAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2779 CCAAGAGATGG
PmaCI 3856 CACGTG CAC^GTG
5788 CACGTG
PmlI 3856 CACGTG CAC^GTG
5788 CACGTG
PvuII 2124 CAGCTG CAG^CTG
6340 CAGCTG
SacI 1662 GAGCTC GAGCT^C
3194 GAGCTC
SmaI 157 CCCGGG CCC^GGG
189 CCCGGG
SspI 5661 AATATT AAT^ATT
6283 AATATT
Van91I 621 CCAAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2779 CCAAGAGATGG
XmaCI 157 CCCGGG C^CCGGG
189 CCCGGG
XmaI 157 CCCGGG C^CCGGG
189 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflII AflIII AluI AlwI AlwNI
ApoI AseI AsnI Asp700I AspHI
AvaI AvaII BalI BanII BbsI
BbvI BciVI BcnI BfaI BfrI
BmyI BpmI BpuAI BsaAI BsaBI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BspLU11I BspMI
BsrBI BsrI BssKI BssSI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI EaeI
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PshBI RcaI RsaI SapI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XhoII XmnI