##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
Acc65I AccIII AclI AgeI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspI AvrII BbeI BbrPI BcgI
BclI BlnI BlpI Bpu1102I BseAI
BsiEI BsiWI BspDI BspEI BspHI
BspLU11I BsrFI Bst1107I BstBI BstEII
BstPI BstZ17I CelII Cfr10I ClaI
CpoI DraI EagI EclXI Eco47III
Eco52I EcoO65I EcoRV EcoT22I FbaI
FseI HincII HindII HpaI KasI
KpnI MluI MroI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI Sse8387I
SwaI Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 1147 GACGTC GACGT^C
AcsI 4175 GAATTC [GA]^AATT[CT]
AflII 334 CTTAAG C^TTAAG
ApoI 4175 GAATTC [GA]^AATT[CT]
AviII 524 TGCGCA TGC^GCA
BamHI 859 GGATCC G^GATCC
BanI 1047 GGCACC G^G[CT][GA]CC
BfrI 334 CTTAAG C^TTAAG
BsaAI 775 TACGTG [CT]AC^GT[GA]
BsaBI 190 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1409 GAGACC GGTCTC(1/5)
BsaWI 2253 TCCGGT [AT]^CCGG[AT]
BsrDI 840 CATTGC GCAATG(2/0)
BssSI 1100 CACGAG CACGAG(-5/-1)
DraIII 3362 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1887 GACAAGAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 4175 GAATTC G^AATTC
FspI 524 TGCGCA TGC^GCA
HaeII 26 GGCGCT [GA]GCGC^[CT]
HgaI 590 GCGTC GACGC(5/10)
KspI 22 CCGCGG CCGC^GG
MamI 190 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3903 CAATTG C^AATTG
MunI 3903 CAATTG C^AATTG
NdeI 2504 CATATG CA^TATG
PflMI 2617 CCAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 22 CCGCGG CCGC^GG
SmaI 3407 CCCGGG CCC^GGG
SpeI 1202 ACTAGT A^CTAGT
SphI 3167 GCATGC GCATG^C
SspI 4197 AATATT AAT^ATT
Van91I 2617 CCAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1383 TCTAGA T^CTAGA
XmaCI 3407 CCCGGG C^CCGGG
XmaI 3407 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 1119 GTCTAC GT^[AC][GT]AC
3426 GTAGAC
AcyI 589 GGCGTC G[GA]^CG[CT]C
1147 GACGTC
AflIII 776 ACGTGT A^C[GA][CT]GT
3109 ACGTGT
AhdI 1081 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3118 GACTCCATGTC
Alw44I 1506 GTGCAC G^TGCAC
3888 GTGCAC
ApaLI 1506 GTGCAC G^TGCAC
3888 GTGCAC
AspEI 1081 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3118 GACTCCATGTC
AvaI 84 CCCGAG C^[CT]CG[GA]G
3407 CCCGGG
BalI 371 TGGCCA TGG^CCA
4095 TGGCCA
BglII 1837 AGATCT A^GATCT
3500 AGATCT
BsaHI 589 GGCGTC G[GA]^CG[CT]C
1147 GACGTC
BsmBI 3197 CGTCTC CGTCTC(1/5)
3310 CGTCTC
BsmI 1605 GAATGC GAATGC(1/-1)
3067 GAATGC
BsoBI 84 CCCGAG C^[CT]CG[GA]G
3407 CCCGGG
Bsp1407I 3077 TGTACA T^GTACA
4211 TGTACA
BsrGI 3077 TGTACA T^GTACA
4211 TGTACA
BssHII 76 GCGCGC G^CGCGC
78 GCGCGC
Eam1105I 1081 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3118 GACTCCATGTC
Hin1I 589 GGCGTC G[GA]^CG[CT]C
1147 GACGTC
HindIII 331 AAGCTT A^AGCTT
2456 AAGCTT
MluNI 371 TGGCCA TGG^CCA
4095 TGGCCA
MscI 371 TGGCCA TGG^CCA
4095 TGGCCA
SacI 3147 GAGCTC GAGCT^C
3603 GAGCTC
SspBI 3077 TGTACA T^GTACA
4211 TGTACA
TfiI 2421 GATTC G^A[AT]TC
4150 GATTC
Tsp509I 3904 AATT ^AATT
4176 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaII
BanII BbsI BbvCI BbvI BciVI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrBI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI Tru9I TseI Tsp45I TspRI
TthHB8I XcmI XhoII