##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
Acc65I AccI AclI AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AviII
BamHI BbeI BbrPI BcgI BlpI
Bpu1102I BsaBI BseRI BsiEI BsiWI
BspDI BsrBI BssHII BssSI Bst1107I
BstZ17I Bsu36I CelII ClaI CpoI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoRV FseI FspI HindIII HpaI
KasI KpnI MamI MfeI MluI
MunI NarI NcoI NdeI NheI
NotI NruI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
Psp1406I PvuI RsrII SalI SbfI
SexAI SfiI SfoI SgrAI SmaI
SphI Sse8387I StuI Tth111I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 196 GACGTC GACGT^C
AseI 2765 ATTAAT AT^TAAT
AsnI 2765 ATTAAT AT^TAAT
AvaI 1121 CTCGGG C^[CT]CG[GA]G
AvaII 1042 GGTCC G^G[AT]CC
AvrII 1731 CCTAGG C^CTAGG
BanI 847 GGTGCC G^G[CT][GA]CC
BbvCI 600 GCTGAGG CCTCAGC(-5/-2)
BciVI 920 GGATAC GTATCC(6/5)
BclI 2789 TGATCA T^GATCA
BglI 71 GCCACCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 524 AGATCT A^GATCT
BlnI 1731 CCTAGG C^CTAGG
BsaAI 2858 TACGTA [CT]AC^GT[GA]
BsaI 179 GAGACC GGTCTC(1/5)
BsgI 52 CTGCAC GTGCAG(16/14)
BsmFI 661 GGGAC GGGAC(10/14)
BsoBI 1121 CTCGGG C^[CT]CG[GA]G
Bsp1407I 2331 TGTACA T^GTACA
BspHI 1036 TCATGA T^CATGA
BsrGI 2331 TGTACA T^GTACA
BstBI 1954 TTCGAA TT^CGAA
BstEII 1371 GGTGACC G^GT[ACGT]ACC
BstPI 1371 GGTGACC G^GT[ACGT]ACC
EcoO65I 1371 GGTGACC G^GT[ACGT]ACC
EcoRI 1455 GAATTC G^AATTC
EcoT22I 532 ATGCAT ATGCA^T
FbaI 2789 TGATCA T^GATCA
HgaI 68 GACGC GACGC(5/10)
HincII 65 GTTGAC GT[CT]^[GA]AC
HindII 65 GTTGAC GT[CT]^[GA]AC
NsiI 532 ATGCAT ATGCA^T
NspV 1954 TTCGAA TT^CGAA
PflMI 825 CCACTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 227 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2765 ATTAAT AT^TAAT
PstI 1159 CTGCAG CTGCA^G
PvuII 958 CAGCTG CAG^CTG
RcaI 1036 TCATGA T^CATGA
ScaI 1609 AGTACT AGT^ACT
SfuI 1954 TTCGAA TT^CGAA
SnaBI 2858 TACGTA TAC^GTA
SspBI 2331 TGTACA T^GTACA
SwaI 3406 ATTTAAAT ATTT^AAAT
Van91I 825 CCACTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 627 CCAAACTCACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 9 CGCG CG^CG
95 CGCG
AccIII 220 TCCGGA T^CCGGA
748 TCCGGA
AcyI 68 GACGCC G[GA]^CG[CT]C
196 GACGTC
AflIII 1699 ACATGT A^C[GA][CT]GT
3003 ACATGT
AlwI 1481 GATCC GGATC(4/5)
1324 GGATC
BanII 1118 GAGCTC G[GA]GC[CT]^C
1548 GAGCTC
BpmI 852 CTGGAG CTGGAG(16/14)
3055 CTGGAG
BsaHI 68 GACGCC G[GA]^CG[CT]C
196 GACGTC
BseAI 220 TCCGGA T^CCGGA
748 TCCGGA
BsmBI 198 CGTCTC CGTCTC(1/5)
35 GAGACG
BsmI 938 GAATGC GAATGC(1/-1)
2900 GCATTC
BspEI 220 TCCGGA T^CCGGA
748 TCCGGA
BspLU11I 1699 ACATGT A^CATGT
3003 ACATGT
BspMI 56 ACCTGC ACCTGC(4/8)
2027 ACCTGC
BsrDI 942 GCAATG GCAATG(2/0)
2094 GCAATG
BstAPI 1925 GCAGACTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2094 GCAATGATTGC
BstUI 9 CGCG CG^CG
95 CGCG
BstXI 844 CCAGGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1361 CCAGTTGGATGG
Cfr13I 78 GGGCC G^G[ACGT]CC
1042 GGTCC
DsaI 8 CCGCGG C^C[GA][CT]GG
94 CCGCGG
EcoNI 1276 CCTACTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1853 CCTCACCAAGG
HaeII 40 GGCGCT [GA]GCGC^[CT]
98 GGCGCT
Hin1I 68 GACGCC G[GA]^CG[CT]C
196 GACGTC
KspI 8 CCGCGG CCGC^GG
94 CCGCGG
MroI 220 TCCGGA T^CCGGA
748 TCCGGA
MvnI 9 CGCG CG^CG
95 CGCG
NaeI 80 GCCGGC GCC^GGC
189 GCCGGC
NgoMIV 80 GCCGGC G^CCGGC
189 GCCGGC
SacI 1118 GAGCTC GAGCT^C
1548 GAGCTC
SacII 8 CCGCGG CCGC^GG
94 CCGCGG
SapI 1545 GAAGAGC GCTCTTC(1/4)
1950 GCTCTTC
Sau96I 78 GGGCC G^G[ACGT]CC
1042 GGTCC
SpeI 2336 ACTAGT A^CTAGT
3300 ACTAGT
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwNI ApoI Asp700I AspHI BalI
BbsI BbvI BcnI BfaI BfrI
BmyI BpuAI BsaJI BsaWI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr10I DdeI DpnII
DraI EaeI EarI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI