##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
AhdI AocI AscI AseI AsnI
Asp718I AspEI AviII BcgI BciVI
BfrI BsiWI BsmBI BspDI BspHI
BspLU11I BssHII Bst1107I BstZ17I Bsu36I
ClaI CpoI DraI Eam1105I Eco81I
EcoRI EcoRV FseI FspI KpnI
KspI MluI NdeI NheI NotI
NruI PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SgrAI SnaBI
Sse8387I SspI StuI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 3129 GACGTC GACGT^C
AcsI 1753 AAATTT [GA]^AATT[CT]
AflIII 1534 ACGTGT A^C[GA][CT]GT
Alw44I 1392 GTGCAC G^TGCAC
ApaLI 1392 GTGCAC G^TGCAC
ApoI 1753 AAATTT [GA]^AATT[CT]
Asp700I 62 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 627 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3159 CCTAGG C^CTAGG
BamHI 778 GGATCC G^GATCC
BbeI 19 GGCGCC GGCGC^C
BclI 994 TGATCA T^GATCA
BglI 3328 GCCCCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3159 CCTAGG C^CTAGG
BsaBI 540 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrDI 4179 CATTGC GCAATG(2/0)
BstBI 242 TTCGAA TT^CGAA
BstEII 1512 GGTGACC G^GT[ACGT]ACC
BstPI 1512 GGTGACC G^GT[ACGT]ACC
DraIII 2139 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1512 GGTGACC G^GT[ACGT]ACC
EcoT22I 3998 ATGCAT ATGCA^T
FbaI 994 TGATCA T^GATCA
HgaI 2608 GACGC GACGC(5/10)
HindIII 1783 AAGCTT A^AGCTT
HpaI 3915 GTTAAC GTT^AAC
KasI 19 GGCGCC G^GCGCC
MamI 540 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2555 CAATTG C^AATTG
MunI 2555 CAATTG C^AATTG
NaeI 3036 GCCGGC GCC^GGC
NarI 19 GGCGCC GG^CGCC
NgoMIV 3036 GCCGGC G^CCGGC
NsiI 3998 ATGCAT ATGCA^T
NspV 242 TTCGAA TT^CGAA
PflFI 627 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 3043 GGGACCC [GA]G^G[AT]CC[CT]
SexAI 864 ACCAGGT A^CC[AT]GGT
SfoI 19 GGCGCC GGC^GCC
SfuI 242 TTCGAA TT^CGAA
SpeI 237 ACTAGT A^CTAGT
Tth111I 627 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 917 TCTAGA T^CTAGA
XmnI 62 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 2835 CGCG CG^CG
3015 CGCG
AccIII 1110 TCCGGA T^CCGGA
2629 TCCGGA
Aor51HI 907 AGCGCT AGC^GCT
3234 AGCGCT
BbvCI 1355 CCTCAGC CCTCAGC(-5/-2)
3230 CCTCAGC
BsaI 795 GAGACC GGTCTC(1/5)
1344 GAGACC
BseAI 1110 TCCGGA T^CCGGA
2629 TCCGGA
BsiEI 1549 CGGCCG CG[GA][CT]^CG
3038 CGGCCG
BsmI 329 GAATGC GAATGC(1/-1)
1484 GAATGC
Bsp1407I 293 TGTACA T^GTACA
1657 TGTACA
BspEI 1110 TCCGGA T^CCGGA
2629 TCCGGA
BsrGI 293 TGTACA T^GTACA
1657 TGTACA
BssSI 447 CTCGTG CACGAG(-5/-1)
2002 CTCGTG
BstUI 2835 CGCG CG^CG
3015 CGCG
BstXI 725 CCAGCATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1462 CCACTGACGTGG
EagI 1549 CGGCCG C^GGCCG
3038 CGGCCG
EarI 2784 CTCTTC CTCTTC(1/4)
4033 GAAGAG
EclXI 1549 CGGCCG C^GGCCG
3038 CGGCCG
Eco47III 907 AGCGCT AGC^GCT
3234 AGCGCT
Eco52I 1549 CGGCCG C^GGCCG
3038 CGGCCG
Ksp632I 2784 CTCTTC CTCTTC(1/4)
4033 GAAGAG
MroI 1110 TCCGGA T^CCGGA
2629 TCCGGA
MvnI 2835 CGCG CG^CG
3015 CGCG
SphI 1003 GCATGC GCATG^C
3794 GCATGC
SspBI 293 TGTACA T^GTACA
1657 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BanI BanII BbrPI BbsI
BbvI BcnI BfaI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI