##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AatII Acc65I AccIII AflII AhdI
AscI AseI AsnI Asp718I AspEI
AspI AviII BfrI BseAI BsiWI
BspDI BspEI BspHI BstBI BstXI
ClaI CpoI Eam1105I FseI FspI
KpnI MroI NheI NotI NruI
NspV PacI PaeR7I PflFI PmeI
PshBI PvuI RcaI RsrII SexAI
SfiI SfuI SgrAI SnaBI SwaI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AclI 669 AACGTT AA^CGTT
AgeI 2545 ACCGGT A^CCGGT
AvrII 6524 CCTAGG C^CTAGG
BbrPI 383 CACGTG CAC^GTG
BbsI 1059 GAAGAC GAAGAC(2/6)
BbvCI 6050 GCTGAGG CCTCAGC(-5/-2)
BcgI 7213 GCACAGCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 6524 CCTAGG C^CTAGG
BpuAI 1059 GAAGAC GAAGAC(2/6)
BsaBI 689 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrBI 1847 GAGCGG CCGCTC(-3/-3)
Bst1107I 6678 GTATAC GTA^TAC
BstEII 3183 GGTAACC G^GT[ACGT]ACC
BstPI 3183 GGTAACC G^GT[ACGT]ACC
BstZ17I 6678 GTATAC GTA^TAC
EcoO65I 3183 GGTAACC G^GT[ACGT]ACC
EcoRV 4784 GATATC GAT^ATC
MamI 689 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 5110 ACGCGT A^CGCGT
NaeI 5637 GCCGGC GCC^GGC
NdeI 127 CATATG CA^TATG
NgoMIV 5637 GCCGGC G^CCGGC
PinAI 2545 ACCGGT A^CCGGT
PmaCI 383 CACGTG CAC^GTG
PmlI 383 CACGTG CAC^GTG
Psp1406I 669 AACGTT AA^CGTT
SalI 4021 GTCGAC G^TCGAC
SpeI 3495 ACTAGT A^CTAGT
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 4021 GTCGAC GT^[AC][GT]AC
6678 GTATAC
AocI 508 CCTGAGG CC^T[ACGT]AGG
2071 CCTCAGG
Aor51HI 1957 AGCGCT AGC^GCT
7217 AGCGCT
ApaI 5097 GGGCCC GGGCC^C
5568 GGGCCC
BamHI 166 GGATCC G^GATCC
3549 GGATCC
BciVI 3918 GGATAC GTATCC(6/5)
5964 GGATAC
BsaWI 2394 ACCGGA [AT]^CCGG[AT]
2545 ACCGGT
BsmBI 956 CGTCTC CGTCTC(1/5)
4558 CGTCTC
BsmI 581 GAATGC GAATGC(1/-1)
4295 GAATGC
BspLU11I 2279 ACATGT A^CATGT
6751 ACATGT
Bsu36I 508 CCTGAGG CC^T[ACGT]AGG
2071 CCTCAGG
DraIII 1533 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
4521 CACTCGGTG
DrdI 1029 GACAGAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7345 GACCACCTTGTC
Eco47III 1957 AGCGCT AGC^GCT
7217 AGCGCT
Eco81I 508 CCTGAGG CC^T[ACGT]AGG
2071 CCTCAGG
EcoRI 2957 GAATTC G^AATTC
5881 GAATTC
HindIII 461 AAGCTT A^AGCTT
1269 AAGCTT
HpaI 1292 GTTAAC GTT^AAC
2010 GTTAAC
MfeI 1738 CAATTG C^AATTG
6784 CAATTG
MunI 1738 CAATTG C^AATTG
6784 CAATTG
PshAI 3589 GACATAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5180 GACCCCCGTC
SapI 1668 GAAGAGC GCTCTTC(1/4)
2138 GCTCTTC
SbfI 2233 CCTGCAGG CCTGCA^GG
6080 CCTGCAGG
ScaI 1450 AGTACT AGT^ACT
5311 AGTACT
Sse8387I 2233 CCTGCAGG CCTGCA^GG
6080 CCTGCAGG
XbaI 3656 TCTAGA T^CTAGA
6833 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 157 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
ApaLI ApoI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbeI
BbvI BclI BcnI BfaI BglI
BglII BlpI BmyI BpmI Bpu1102I
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrDI BsrFI BsrGI BsrI
BssHII BssKI BssSI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EagI
EarI EclXI Eco52I EcoNI EcoO109I
EcoRII EcoT14I EcoT22I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI SphI SspBI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI XmnI