################################## # NO CUT ENZYMES ( 47 enzymes) # ################################## AatII Acc65I AccIII AflII AhdI AscI AseI AsnI Asp718I AspEI AspI AviII BfrI BseAI BsiWI BspDI BspEI BspHI BstBI BstXI ClaI CpoI Eam1105I FseI FspI KpnI MroI NheI NotI NruI NspV PacI PaeR7I PflFI PmeI PshBI PvuI RcaI RsrII SexAI SfiI SfuI SgrAI SnaBI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### AclI 669 AACGTT AA^CGTT AgeI 2545 ACCGGT A^CCGGT AvrII 6524 CCTAGG C^CTAGG BbrPI 383 CACGTG CAC^GTG BbsI 1059 GAAGAC GAAGAC(2/6) BbvCI 6050 GCTGAGG CCTCAGC(-5/-2) BcgI 7213 GCACAGCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 6524 CCTAGG C^CTAGG BpuAI 1059 GAAGAC GAAGAC(2/6) BsaBI 689 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsrBI 1847 GAGCGG CCGCTC(-3/-3) Bst1107I 6678 GTATAC GTA^TAC BstEII 3183 GGTAACC G^GT[ACGT]ACC BstPI 3183 GGTAACC G^GT[ACGT]ACC BstZ17I 6678 GTATAC GTA^TAC EcoO65I 3183 GGTAACC G^GT[ACGT]ACC EcoRV 4784 GATATC GAT^ATC MamI 689 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 5110 ACGCGT A^CGCGT NaeI 5637 GCCGGC GCC^GGC NdeI 127 CATATG CA^TATG NgoMIV 5637 GCCGGC G^CCGGC PinAI 2545 ACCGGT A^CCGGT PmaCI 383 CACGTG CAC^GTG PmlI 383 CACGTG CAC^GTG Psp1406I 669 AACGTT AA^CGTT SalI 4021 GTCGAC G^TCGAC SpeI 3495 ACTAGT A^CTAGT ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccI 4021 GTCGAC GT^[AC][GT]AC 6678 GTATAC AocI 508 CCTGAGG CC^T[ACGT]AGG 2071 CCTCAGG Aor51HI 1957 AGCGCT AGC^GCT 7217 AGCGCT ApaI 5097 GGGCCC GGGCC^C 5568 GGGCCC BamHI 166 GGATCC G^GATCC 3549 GGATCC BciVI 3918 GGATAC GTATCC(6/5) 5964 GGATAC BsaWI 2394 ACCGGA [AT]^CCGG[AT] 2545 ACCGGT BsmBI 956 CGTCTC CGTCTC(1/5) 4558 CGTCTC BsmI 581 GAATGC GAATGC(1/-1) 4295 GAATGC BspLU11I 2279 ACATGT A^CATGT 6751 ACATGT Bsu36I 508 CCTGAGG CC^T[ACGT]AGG 2071 CCTCAGG DraIII 1533 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG 4521 CACTCGGTG DrdI 1029 GACAGAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 7345 GACCACCTTGTC Eco47III 1957 AGCGCT AGC^GCT 7217 AGCGCT Eco81I 508 CCTGAGG CC^T[ACGT]AGG 2071 CCTCAGG EcoRI 2957 GAATTC G^AATTC 5881 GAATTC HindIII 461 AAGCTT A^AGCTT 1269 AAGCTT HpaI 1292 GTTAAC GTT^AAC 2010 GTTAAC MfeI 1738 CAATTG C^AATTG 6784 CAATTG MunI 1738 CAATTG C^AATTG 6784 CAATTG PshAI 3589 GACATAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 5180 GACCCCCGTC SapI 1668 GAAGAGC GCTCTTC(1/4) 2138 GCTCTTC SbfI 2233 CCTGCAGG CCTGCA^GG 6080 CCTGCAGG ScaI 1450 AGTACT AGT^ACT 5311 AGTACT Sse8387I 2233 CCTGCAGG CCTGCA^GG 6080 CCTGCAGG XbaI 3656 TCTAGA T^CTAGA 6833 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 157 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI ApaLI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbeI BbvI BclI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrDI BsrFI BsrGI BsrI BssHII BssKI BssSI BstAPI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EagI EarI EclXI Eco52I EcoNI EcoO109I EcoRII EcoT14I EcoT22I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI SphI SspBI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI XmnI