##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AclI AhdI AscI AseI AsnI
AspEI AvrII BamHI BciVI BclI
BlnI BsaBI BsiWI BsmI BspDI
BspHI BsrDI BstBI BstEII BstPI
ClaI CpoI DraI DrdI Eam1105I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI MamI MfeI MluI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SbfI ScaI SexAI SfiI SfuI
SnaBI SpeI SphI Sse8387I SspI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 2076 GACGTC GACGT^C
Acc65I 807 GGTACC G^GTACC
AccIII 2347 TCCGGA T^CCGGA
AcsI 4298 AAATTC [GA]^AATT[CT]
AflII 3370 CTTAAG C^TTAAG
AgeI 477 ACCGGT A^CCGGT
AocI 2004 CCTGAGG CC^T[ACGT]AGG
Aor51HI 3717 AGCGCT AGC^GCT
ApoI 4298 AAATTC [GA]^AATT[CT]
Asp718I 807 GGTACC G^GTACC
AspI 557 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BfaI 4248 CTAG C^TAG
BfrI 3370 CTTAAG C^TTAAG
BglII 954 AGATCT A^GATCT
BseAI 2347 TCCGGA T^CCGGA
BsiEI 38 CGGCCG CG[GA][CT]^CG
BspEI 2347 TCCGGA T^CCGGA
BspLU11I 2869 ACATGT A^CATGT
BssSI 2585 CTCGTG CACGAG(-5/-1)
Bst1107I 230 GTATAC GTA^TAC
BstYI 954 AGATCT [GA]^GATC[CT]
BstZ17I 230 GTATAC GTA^TAC
Bsu36I 2004 CCTGAGG CC^T[ACGT]AGG
DraIII 415 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 38 CGGCCG C^GGCCG
EclXI 38 CGGCCG C^GGCCG
Eco47III 3717 AGCGCT AGC^GCT
Eco52I 38 CGGCCG C^GGCCG
Eco81I 2004 CCTGAGG CC^T[ACGT]AGG
HindIII 3213 AAGCTT A^AGCTT
HpaI 3521 GTTAAC GTT^AAC
KpnI 807 GGTACC GGTAC^C
MaeI 4248 CTAG C^TAG
MflI 954 AGATCT [GA]^GATC[CT]
MroI 2347 TCCGGA T^CCGGA
PflFI 557 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 477 ACCGGT A^CCGGT
SalI 2073 GTCGAC G^TCGAC
SgrAI 841 CGCCGGTG C[GA]^CCGG[CT]G
StuI 2581 AGGCCT AGG^CCT
TfiI 3974 GATTC G^A[AT]TC
Tth111I 557 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 954 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 230 GTATAC GT^[AC][GT]AC
2073 GTCGAC
Alw44I 481 GTGCAC G^TGCAC
819 GTGCAC
ApaLI 481 GTGCAC G^TGCAC
819 GTGCAC
Asp700I 2120 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3893 GAACCTTTTC
AviII 1741 TGCGCA TGC^GCA
2812 TGCGCA
BbeI 2159 GGCGCC GGCGC^C
2439 GGCGCC
BcgI 1300 GCAGCCAGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3407 GCATCCAGCTCG
BlpI 2537 GCTGAGC GC^T[ACGT]AGC
3151 GCTAAGC
Bpu1102I 2537 GCTGAGC GC^T[ACGT]AGC
3151 GCTAAGC
Bsp1407I 1180 TGTACA T^GTACA
2174 TGTACA
BsrBI 1667 CCGCTC CCGCTC(-3/-3)
3992 GAGCGG
BsrGI 1180 TGTACA T^GTACA
2174 TGTACA
CelII 2537 GCTGAGC GC^T[ACGT]AGC
3151 GCTAAGC
FspI 1741 TGCGCA TGC^GCA
2812 TGCGCA
KasI 2159 GGCGCC G^GCGCC
2439 GGCGCC
NarI 2159 GGCGCC GG^CGCC
2439 GGCGCC
NspI 1471 ACATGC [GA]CATG^[CT]
2869 ACATGT
SacI 444 GAGCTC GAGCT^C
1977 GAGCTC
SfoI 2159 GGCGCC GGC^GCC
2439 GGCGCC
SspBI 1180 TGTACA T^GTACA
2174 TGTACA
XmnI 2120 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3893 GAACCTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbrPI BbsI BbvCI BbvI BcnI
BglI BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssHII BssKI BstAPI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
KspI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SacII SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XmaCI
XmaI