##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AgeI AhdI Alw44I ApaLI ApoI
AscI AspEI AviII AvrII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BfrI BglII BlnI BpuAI BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco52I EcoRI
EcoRV EcoT22I FseI FspI HindIII
KasI KspI MamI MfeI MluI
MroI MunI NarI NcoI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SfiI SfoI
SfuI SnaBI SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 984 GGTACC G^GTACC
AccI 1228 GTCGAC GT^[AC][GT]AC
AccII 1714 CGCG CG^CG
AcyI 3874 GACGCC G[GA]^CG[CT]C
AflIII 1537 ACATGT A^C[GA][CT]GT
Aor51HI 3676 AGCGCT AGC^GCT
AseI 1779 ATTAAT AT^TAAT
AsnI 1779 ATTAAT AT^TAAT
Asp700I 1401 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 984 GGTACC G^GTACC
AspI 2838 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BclI 220 TGATCA T^GATCA
BsaHI 3874 GACGCC G[GA]^CG[CT]C
BsmBI 3038 GAGACG CGTCTC(1/5)
Bsp1407I 2773 TGTACA T^GTACA
BspHI 1760 TCATGA T^CATGA
BspLU11I 1537 ACATGT A^CATGT
BsrBI 3141 GAGCGG CCGCTC(-3/-3)
BsrGI 2773 TGTACA T^GTACA
BstEII 386 GGTGACC G^GT[ACGT]ACC
BstPI 386 GGTGACC G^GT[ACGT]ACC
BstUI 1714 CGCG CG^CG
DraIII 2634 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 3676 AGCGCT AGC^GCT
EcoO65I 386 GGTGACC G^GT[ACGT]ACC
FbaI 220 TGATCA T^GATCA
Hin1I 3874 GACGCC G[GA]^CG[CT]C
HpaI 947 GTTAAC GTT^AAC
KpnI 984 GGTACC GGTAC^C
MvnI 1714 CGCG CG^CG
NdeI 4117 CATATG CA^TATG
NheI 3951 GCTAGC G^CTAGC
PflFI 2838 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1284 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1779 ATTAAT AT^TAAT
RcaI 1760 TCATGA T^CATGA
SalI 1228 GTCGAC G^TCGAC
SbfI 2741 CCTGCAGG CCTGCA^GG
ScaI 3259 AGTACT AGT^ACT
SexAI 1082 ACCTGGT A^CC[AT]GGT
SgrAI 2809 CACCGGCG C[GA]^CCGG[CT]G
SmaI 2884 CCCGGG CCC^GGG
SmlI 2441 CTTGAG C^T[CT][GA]AG
SpeI 54 ACTAGT A^CTAGT
SphI 3685 GCATGC GCATG^C
Sse8387I 2741 CCTGCAGG CCTGCA^GG
SspBI 2773 TGTACA T^GTACA
StuI 2940 AGGCCT AGG^CCT
Tth111I 2838 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1235 TCTAGA T^CTAGA
XmaCI 2884 CCCGGG C^CCGGG
XmaI 2884 CCCGGG C^CCGGG
XmnI 1401 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
BsrDI 1311 CATTGC GCAATG(2/0)
2224 GCAATG
BssSI 3614 CACGAG CACGAG(-5/-1)
2637 CTCGTG
BstXI 535 CCATTCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2092 CCAATGGCTTGG
EcoNI 1119 CCTGACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2916 CCTGCCACAGG
MseI 948 TTAA T^TAA
1780 TTAA
NaeI 189 GCCGGC GCC^GGC
1893 GCCGGC
NgoMIV 189 GCCGGC G^CCGGC
1893 GCCGGC
PstI 2742 CTGCAG CTGCA^G
3541 CTGCAG
SapI 3405 GAAGAGC GCTCTTC(1/4)
1404 GCTCTTC
Tru9I 948 TTAA T^TAA
1780 TTAA
Tsp509I 85 AATT ^AATT
3226 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
BalI BamHI BanI BanII BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I Van91I XcmI XhoII