##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
Acc65I AclI AflII AgeI AscI
AseI AsnI Asp718I AvrII BamHI
BcgI BclI BfrI BlnI BsaBI
Bsp1407I BspDI BspLU11I BsrGI Bst1107I
BstZ17I ClaI CpoI DrdI EcoNI
EcoRV FbaI FseI HincII HindII
HpaI KpnI MamI MfeI MluI
MunI NdeI NheI NotI NruI
PacI PinAI PmeI PshAI PshBI
Psp1406I RsrII SalI SbfI ScaI
SgrAI SnaBI SpeI Sse8387I SspBI
SwaI XbaI XcmI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 2450 GACGTC GACGT^C
AccI 4289 GTCTAC GT^[AC][GT]AC
AflIII 1667 ACGTGT A^C[GA][CT]GT
AhdI 497 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1985 GGATC GGATC(4/5)
Aor51HI 2040 AGCGCT AGC^GCT
Asp700I 66 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 497 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2746 TGCGCA TGC^GCA
BalI 2108 TGGCCA TGG^CCA
BbrPI 1666 CACGTG CAC^GTG
BbvCI 970 CCTCAGC CCTCAGC(-5/-2)
BciVI 1960 GTATCC GTATCC(6/5)
BglII 3666 AGATCT A^GATCT
BsaAI 1666 CACGTG [CT]AC^GT[GA]
BsiWI 1827 CGTACG C^GTACG
BspHI 2656 TCATGA T^CATGA
BsrDI 1580 CATTGC GCAATG(2/0)
BstAPI 2568 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1472 TTCGAA TT^CGAA
BstXI 1132 CCACCCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 3666 AGATCT [GA]^GATC[CT]
DraI 3661 TTTAAA TTT^AAA
DraIII 2514 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 497 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2040 AGCGCT AGC^GCT
EcoRI 3760 GAATTC G^AATTC
EcoT22I 2258 ATGCAT ATGCA^T
FspI 2746 TGCGCA TGC^GCA
HindIII 67 AAGCTT A^AGCTT
KspI 3196 CCGCGG CCGC^GG
MflI 3666 AGATCT [GA]^GATC[CT]
MluNI 2108 TGGCCA TGG^CCA
MscI 2108 TGGCCA TGG^CCA
NcoI 2626 CCATGG C^CATGG
NsiI 2258 ATGCAT ATGCA^T
NspV 1472 TTCGAA TT^CGAA
PaeR7I 2790 CTCGAG C^TCGAG
PflMI 4181 CCAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1666 CACGTG CAC^GTG
PmlI 1666 CACGTG CAC^GTG
PvuI 3098 CGATCG CGAT^CG
PvuII 2730 CAGCTG CAG^CTG
RcaI 2656 TCATGA T^CATGA
SacII 3196 CCGCGG CCGC^GG
SapI 1842 GAAGAGC GCTCTTC(1/4)
SexAI 2086 ACCTGGT A^CC[AT]GGT
SfuI 1472 TTCGAA TT^CGAA
StuI 869 AGGCCT AGG^CCT
Van91I 4181 CCAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2790 CTCGAG C^TCGAG
XhoII 3666 AGATCT [GA]^GATC[CT]
XmnI 66 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 2560 TCCGGA T^CCGGA
2903 TCCGGA
AcsI 1349 AAATTT [GA]^AATT[CT]
3760 GAATTC
Alw44I 2566 GTGCAC G^TGCAC
3891 GTGCAC
AlwNI 3556 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
4182 CAGTTGCTG
ApaLI 2566 GTGCAC G^TGCAC
3891 GTGCAC
ApoI 1349 AAATTT [GA]^AATT[CT]
3760 GAATTC
AspI 2660 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3458 GACTCTGTC
BbsI 741 GTCTTC GAAGAC(2/6)
2140 GTCTTC
BlpI 2298 GCTCAGC GC^T[ACGT]AGC
4169 GCTGAGC
Bpu1102I 2298 GCTCAGC GC^T[ACGT]AGC
4169 GCTGAGC
BpuAI 741 GTCTTC GAAGAC(2/6)
2140 GTCTTC
BseAI 2560 TCCGGA T^CCGGA
2903 TCCGGA
BspEI 2560 TCCGGA T^CCGGA
2903 TCCGGA
BsrFI 1265 GCCGGC [GA]^CCGG[CT]
2810 GCCGGC
BssHII 1689 GCGCGC G^CGCGC
3330 GCGCGC
BssSI 4124 CACGAG CACGAG(-5/-1)
3273 CTCGTG
BstEII 333 GGTCACC G^GT[ACGT]ACC
1801 GGTCACC
BstPI 333 GGTCACC G^GT[ACGT]ACC
1801 GGTCACC
CelII 2298 GCTCAGC GC^T[ACGT]AGC
4169 GCTGAGC
Cfr10I 1265 GCCGGC [GA]^CCGG[CT]
2810 GCCGGC
EcoO65I 333 GGTCACC G^GT[ACGT]ACC
1801 GGTCACC
MroI 2560 TCCGGA T^CCGGA
2903 TCCGGA
MslI 2627 CATGGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4124 CACGAGAATG
NaeI 1265 GCCGGC GCC^GGC
2810 GCCGGC
NgoMIV 1265 GCCGGC G^CCGGC
2810 GCCGGC
PflFI 2660 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3458 GACTCTGTC
SfiI 1853 GGCCCCAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3281 GGCCGTGGTGGCC
SmlI 2790 CTCGAG C^T[CT][GA]AG
4015 CTCAAG
SphI 2692 GCATGC GCATG^C
3259 GCATGC
SspI 1885 AATATT AAT^ATT
1903 AATATT
Tth111I 2660 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3458 GACTCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AocI ApaI AspHI AvaI AvaII
BanI BanII BbeI BbvI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstUI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EarI EclXI Eco52I
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XmaCI
XmaI