##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AclI AcsI AflII AgeI AhdI
ApoI AscI AseI AsnI Asp700I
AspEI AvrII BamHI BbsI BcgI
BciVI BclI BfrI BglII BlnI
BpuAI BsaBI BsiEI BsiWI BspDI
BspHI BspLU11I BsrDI Bst1107I BstZ17I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco52I EcoRI EcoRV
EcoT22I FbaI FseI HpaI MamI
MluI NdeI NheI NotI NruI
NsiI PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SgrAI SnaBI SpeI
SspI SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 2482 GTCTAC GT^[AC][GT]AC
AccIII 158 TCCGGA T^CCGGA
AflIII 1591 ACGTGT A^C[GA][CT]GT
Aor51HI 3716 AGCGCT AGC^GCT
AspI 1880 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1437 GGCGCC GGCGC^C
BbrPI 231 CACGTG CAC^GTG
BbvCI 829 GCTGAGG CCTCAGC(-5/-2)
BlpI 2114 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2114 GCTGAGC GC^T[ACGT]AGC
BsaWI 158 TCCGGA [AT]^CCGG[AT]
BseAI 158 TCCGGA T^CCGGA
BsmBI 1870 GAGACG CGTCTC(1/5)
BsmI 3565 GAATGC GAATGC(1/-1)
BspEI 158 TCCGGA T^CCGGA
BssHII 37 GCGCGC G^CGCGC
BstBI 3676 TTCGAA TT^CGAA
CelII 2114 GCTGAGC GC^T[ACGT]AGC
Eco47III 3716 AGCGCT AGC^GCT
HincII 2582 GTTGAC GT[CT]^[GA]AC
HindII 2582 GTTGAC GT[CT]^[GA]AC
HindIII 2809 AAGCTT A^AGCTT
KasI 1437 GGCGCC G^GCGCC
MfeI 1269 CAATTG C^AATTG
MroI 158 TCCGGA T^CCGGA
MunI 1269 CAATTG C^AATTG
NaeI 2352 GCCGGC GCC^GGC
NarI 1437 GGCGCC GG^CGCC
NcoI 3124 CCATGG C^CATGG
NgoMIV 2352 GCCGGC G^CCGGC
NspV 3676 TTCGAA TT^CGAA
PflFI 1880 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2707 CCAGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 231 CACGTG CAC^GTG
PmlI 231 CACGTG CAC^GTG
ScaI 3406 AGTACT AGT^ACT
SexAI 3698 ACCAGGT A^CC[AT]GGT
SfiI 3319 GGCCGTGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1437 GGCGCC GGC^GCC
SfuI 3676 TTCGAA TT^CGAA
SphI 1701 GCATGC GCATG^C
Tth111I 1880 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2707 CCAGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2720 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AatII 652 GACGTC GACGT^C
1872 GACGTC
Acc65I 1089 GGTACC G^GTACC
3536 GGTACC
Alw44I 1528 GTGCAC G^TGCAC
2213 GTGCAC
ApaLI 1528 GTGCAC G^TGCAC
2213 GTGCAC
Asp718I 1089 GGTACC G^GTACC
3536 GGTACC
AvaI 85 CCCGGG C^[CT]CG[GA]G
1128 CCCGGG
AviII 687 TGCGCA TGC^GCA
3015 TGCGCA
BsaAI 231 CACGTG [CT]AC^GT[GA]
2730 TACGTG
BsoBI 85 CCCGGG C^[CT]CG[GA]G
1128 CCCGGG
Bsp1407I 362 TGTACA T^GTACA
2232 TGTACA
BsrFI 642 ACCGGC [GA]^CCGG[CT]
2352 GCCGGC
BsrGI 362 TGTACA T^GTACA
2232 TGTACA
BssSI 1043 CACGAG CACGAG(-5/-1)
3789 CACGAG
BstXI 2907 CCATGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3237 CCAGCTCCCTGG
BstYI 994 AGATCC [GA]^GATC[CT]
2597 AGATCC
Cfr10I 642 ACCGGC [GA]^CCGG[CT]
2352 GCCGGC
DraI 960 TTTAAA TTT^AAA
3972 TTTAAA
FspI 687 TGCGCA TGC^GCA
3015 TGCGCA
HgaI 1161 GACGC GACGC(5/10)
1832 GCGTC
KpnI 1089 GGTACC GGTAC^C
3536 GGTACC
KspI 48 CCGCGG CCGC^GG
585 CCGCGG
MflI 994 AGATCC [GA]^GATC[CT]
2597 AGATCC
MslI 1521 CACAGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1782 CATACAGATG
SacII 48 CCGCGG CCGC^GG
585 CCGCGG
SapI 244 GAAGAGC GCTCTTC(1/4)
624 GCTCTTC
SbfI 630 CCTGCAGG CCTGCA^GG
657 CCTGCAGG
SmaI 85 CCCGGG CCC^GGG
1128 CCCGGG
SmlI 759 CTCAAG C^T[CT][GA]AG
1217 CTTGAG
Sse8387I 630 CCTGCAGG CCTGCA^GG
657 CCTGCAGG
SspBI 362 TGTACA T^GTACA
2232 TGTACA
StuI 1047 AGGCCT AGG^CCT
3327 AGGCCT
XhoII 994 AGATCC [GA]^GATC[CT]
2597 AGATCC
XmaCI 85 CCCGGG C^CCGGG
1128 CCCGGG
XmaI 85 CCCGGG C^CCGGG
1128 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaII BalI BanI BanII BbvI
BcnI BfaI BglI BmyI BpmI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrBI BsrI BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstUI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI