##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
Acc65I AccII AccIII AgeI AhdI
Aor51HI ApaI AscI Asp718I AspEI
AviII AvrII BamHI BbeI BbrPI
BcgI BclI BlnI BlpI Bpu1102I
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspMI BsrBI BsrGI
BssHII BssSI BstEII BstPI BstUI
CelII ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I FauI FbaI FseI FspI
HgaI HindIII HpaI KasI KpnI
KspI MfeI MluI MroI MunI
MvnI NarI NdeI NotI NruI
PinAI PmaCI PmeI PmlI PshAI
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SpeI Sse8387I SspBI StuI
SwaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 2951 GACGTC GACGT^C
AcyI 2951 GACGTC G[GA]^CG[CT]C
AlwNI 745 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 1996 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 3420 TGGCCA TGG^CCA
BanII 1147 GAGCTC G[GA]GC[CT]^C
BbsI 924 GAAGAC GAAGAC(2/6)
BbvCI 2324 CCTCAGC CCTCAGC(-5/-2)
BglI 1109 GCCTCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1882 AGATCT A^GATCT
BpuAI 924 GAAGAC GAAGAC(2/6)
BsaAI 3379 TACGTA [CT]AC^GT[GA]
BsaBI 628 GATATCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2951 GACGTC G[GA]^CG[CT]C
BsaWI 2100 ACCGGA [AT]^CCGG[AT]
BsgI 2226 CTGCAC GTGCAG(16/14)
BspHI 3262 TCATGA T^CATGA
BsrFI 1016 GCCGGC [GA]^CCGG[CT]
Bst1107I 3358 GTATAC GTA^TAC
BstBI 1065 TTCGAA TT^CGAA
BstZ17I 3358 GTATAC GTA^TAC
Cfr10I 1016 GCCGGC [GA]^CCGG[CT]
DsaI 684 CCATGG C^C[GA][CT]GG
EcoRV 628 GATATC GAT^ATC
HaeII 941 AGCGCC [GA]GCGC^[CT]
Hin1I 2951 GACGTC G[GA]^CG[CT]C
HincII 2594 GTTGAC GT[CT]^[GA]AC
HindII 2594 GTTGAC GT[CT]^[GA]AC
MamI 628 GATATCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3420 TGGCCA TGG^CCA
MscI 3420 TGGCCA TGG^CCA
NaeI 1016 GCCGGC GCC^GGC
NcoI 684 CCATGG C^CATGG
NgoMIV 1016 GCCGGC G^CCGGC
NheI 2375 GCTAGC G^CTAGC
NspV 1065 TTCGAA TT^CGAA
PacI 2133 TTAATTAA TTAAT^TAA
PflFI 1996 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 819 CCATGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 3262 TCATGA T^CATGA
SacI 1147 GAGCTC GAGCT^C
SapI 937 GAAGAGC GCTCTTC(1/4)
SfuI 1065 TTCGAA TT^CGAA
SnaBI 3379 TACGTA TAC^GTA
SphI 2690 GCATGC GCATG^C
SspI 2173 AATATT AAT^ATT
Tth111I 1996 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 819 CCATGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3408 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AciI 1014 CCGC CCGC(-3/-1)
726 GCGG
AclI 2485 AACGTT AA^CGTT
3537 AACGTT
Alw44I 309 GTGCAC G^TGCAC
1085 GTGCAC
AocI 2298 CCTGAGG CC^T[ACGT]AGG
2749 CCTGAGG
ApaLI 309 GTGCAC G^TGCAC
1085 GTGCAC
Asp700I 348 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2702 GAAGGGATTC
BanI 2097 GGCACC G^G[CT][GA]CC
3133 GGTGCC
BcnI 500 CCGGG CC^[GC]GG
1283 CCCGG
BsaI 680 GAGACC GGTCTC(1/5)
3447 GGTCTC
BsmI 1404 GCATTC GAATGC(1/-1)
1579 GCATTC
BsrDI 3386 CATTGC GCAATG(2/0)
26 GCAATG
BstYI 1687 AGATCC [GA]^GATC[CT]
1882 AGATCT
Bsu36I 2298 CCTGAGG CC^T[ACGT]AGG
2749 CCTGAGG
CfoI 942 GCGC GCG^C
1243 GCGC
DraII 746 AGGACCT [GA]G^G[ACGT]CC[CT]
2745 AGGACCT
EaeI 497 TGGCCG [CT]^GGCC[GA]
3420 TGGCCA
Eco81I 2298 CCTGAGG CC^T[ACGT]AGG
2749 CCTGAGG
EcoNI 49 CCTTGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3000 CCTGGGAGAGG
EcoO109I 746 AGGACCT [GA]G^G[ACGT]CC[CT]
2745 AGGACCT
HhaI 942 GCGC GCG^C
1243 GCGC
HinP1I 942 GCGC G^CGC
1243 GCGC
MflI 1687 AGATCC [GA]^GATC[CT]
1882 AGATCT
NciI 500 CCGGG CC^[GC]GG
1283 CCCGG
PaeR7I 143 CTCGAG C^TCGAG
515 CTCGAG
PpuMI 746 AGGACCT [GA]G^G[AT]CC[CT]
2745 AGGACCT
Psp1406I 2485 AACGTT AA^CGTT
3537 AACGTT
XhoI 143 CTCGAG C^TCGAG
515 CTCGAG
XhoII 1687 AGATCC [GA]^GATC[CT]
1882 AGATCT
XmnI 348 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2702 GAAGGGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccI AcsI AfaI AflII AflIII
AluI AlwI ApoI AseI AsnI
AspHI AvaI AvaII BbvI BciVI
BfaI BfrI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspLU11I BsrI
BssKI BstAPI BstF5I BstNI BstXI
Cac8I Cfr13I DdeI DpnII DraI
EarI EcoRI EcoRII EcoT14I EcoT22I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NsiI
NspI PleI PshBI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I