##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AgeI AhdI Alw44I ApaLI AscI
Asp718I AspEI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BclI BcnI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BsrBI BsrFI BssHII BssSI
Bst1107I BstEII BstPI BstUI BstZ17I
CelII Cfr10I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco52I EcoO65I
FbaI FseI FspI HapII HpaI
HpaII KasI KpnI KspI MluI
MroI MspA1I MspI MvnI NaeI
NarI NciI NdeI NgoMIV NotI
NruI PacI PaeR7I PflMI PinAI
PmaCI PmlI PshAI PvuI PvuII
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I StuI SwaI
Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AciI 367 GCGG CCGC(-3/-1)
AclI 3929 AACGTT AA^CGTT
AcyI 170 GGCGTC G[GA]^CG[CT]C
AflIII 1019 ACATGT A^C[GA][CT]GT
Aor51HI 892 AGCGCT AGC^GCT
ApaI 478 GGGCCC GGGCC^C
AspI 964 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2451 TGGCCA TGG^CCA
BsaHI 170 GGCGTC G[GA]^CG[CT]C
BseRI 957 GAGGAG GAGGAG(10/8)
BsgI 584 GTGCAG GTGCAG(16/14)
BspHI 147 TCATGA T^CATGA
BspLU11I 1019 ACATGT A^CATGT
BspMI 399 ACCTGC ACCTGC(4/8)
BstAPI 3738 GCATTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1345 TTCGAA TT^CGAA
BstXI 1993 CCAAGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 1076 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2451 TGGCCA [CT]^GGCC[GA]
EarI 674 GAAGAG CTCTTC(1/4)
Eco47III 892 AGCGCT AGC^GCT
EcoNI 796 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1959 GAATTC G^AATTC
EcoRV 2457 GATATC GAT^ATC
EcoT22I 2477 ATGCAT ATGCA^T
FauI 367 GCGGG CCCGC(4/6)
Hin1I 170 GGCGTC G[GA]^CG[CT]C
HincII 218 GTCAAC GT[CT]^[GA]AC
HindII 218 GTCAAC GT[CT]^[GA]AC
Ksp632I 674 GAAGAG CTCTTC(1/4)
MfeI 3382 CAATTG C^AATTG
MluNI 2451 TGGCCA TGG^CCA
MscI 2451 TGGCCA TGG^CCA
MunI 3382 CAATTG C^AATTG
NheI 1703 GCTAGC G^CTAGC
NsiI 2477 ATGCAT ATGCA^T
NspV 1345 TTCGAA TT^CGAA
PflFI 964 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 3929 AACGTT AA^CGTT
PstI 3170 CTGCAG CTGCA^G
RcaI 147 TCATGA T^CATGA
SacI 2373 GAGCTC GAGCT^C
ScaI 2707 AGTACT AGT^ACT
SfuI 1345 TTCGAA TT^CGAA
Tth111I 964 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2379 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AocI 796 CCTGAGG CC^T[ACGT]AGG
3531 CCTAAGG
BanII 478 GGGCCC G[GA]GC[CT]^C
2373 GAGCTC
BcgI 2857 CGACACCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3193 CGAAGTCAGTGC
BciVI 3058 GGATAC GTATCC(6/5)
1593 GTATCC
BpmI 452 CTGGAG CTGGAG(16/14)
952 CTGGAG
BsaBI 1457 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2004 GATCTTTATC
BsaI 509 GAGACC GGTCTC(1/5)
962 GAGACC
BsmI 1654 GAATGC GAATGC(1/-1)
3738 GCATTC
Bsp1407I 325 TGTACA T^GTACA
383 TGTACA
BsrGI 325 TGTACA T^GTACA
383 TGTACA
Bsu36I 796 CCTGAGG CC^T[ACGT]AGG
3531 CCTAAGG
CfoI 442 GCGC GCG^C
893 GCGC
DraII 396 AGGACCT [GA]G^G[ACGT]CC[CT]
2983 GGGTCCC
Eco81I 796 CCTGAGG CC^T[ACGT]AGG
3531 CCTAAGG
EcoO109I 396 AGGACCT [GA]G^G[ACGT]CC[CT]
2983 GGGTCCC
HaeII 441 GGCGCT [GA]GCGC^[CT]
892 AGCGCT
HgaI 1399 GACGC GACGC(5/10)
171 GCGTC
HhaI 442 GCGC GCG^C
893 GCGC
HinP1I 442 GCGC G^CGC
893 GCGC
MamI 1457 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2004 GATCTTTATC
NcoI 538 CCATGG C^CATGG
2448 CCATGG
PmeI 2883 GTTTAAAC GTTT^AAAC
3602 GTTTAAAC
PpuMI 396 AGGACCT [GA]G^G[AT]CC[CT]
2983 GGGTCCC
SfcI 635 CTACAG C^T[GA][CT]AG
3170 CTGCAG
SphI 1715 GCATGC GCATG^C
2070 GCATGC
SspBI 325 TGTACA T^GTACA
383 TGTACA
TaqI 1341 TCGA T^CGA
1346 TCGA
TthHB8I 1341 TCGA T^CGA
1346 TCGA
XcmI 3023 CCAAAACACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3222 CCATTAATATCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AcsI AfaI AflII AluI AlwI
AlwNI ApoI AseI AsnI Asp700I
AspHI AvaII BbsI BbvI BfaI
BfrI BmyI BpuAI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DsaI EcoRII EcoT14I Fnu4HI
FokI HaeIII HindIII HinfI HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PshBI RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI SspI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII XmnI