################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AgeI AhdI Alw44I ApaLI AscI Asp718I AspEI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BclI BcnI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BsrBI BsrFI BssHII BssSI Bst1107I BstEII BstPI BstUI BstZ17I CelII Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco52I EcoO65I FbaI FseI FspI HapII HpaI HpaII KasI KpnI KspI MluI MroI MspA1I MspI MvnI NaeI NarI NciI NdeI NgoMIV NotI NruI PacI PaeR7I PflMI PinAI PmaCI PmlI PshAI PvuI PvuII RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I StuI SwaI Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AciI 367 GCGG CCGC(-3/-1) AclI 3929 AACGTT AA^CGTT AcyI 170 GGCGTC G[GA]^CG[CT]C AflIII 1019 ACATGT A^C[GA][CT]GT Aor51HI 892 AGCGCT AGC^GCT ApaI 478 GGGCCC GGGCC^C AspI 964 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 2451 TGGCCA TGG^CCA BsaHI 170 GGCGTC G[GA]^CG[CT]C BseRI 957 GAGGAG GAGGAG(10/8) BsgI 584 GTGCAG GTGCAG(16/14) BspHI 147 TCATGA T^CATGA BspLU11I 1019 ACATGT A^CATGT BspMI 399 ACCTGC ACCTGC(4/8) BstAPI 3738 GCATTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1345 TTCGAA TT^CGAA BstXI 1993 CCAAGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraIII 1076 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 2451 TGGCCA [CT]^GGCC[GA] EarI 674 GAAGAG CTCTTC(1/4) Eco47III 892 AGCGCT AGC^GCT EcoNI 796 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1959 GAATTC G^AATTC EcoRV 2457 GATATC GAT^ATC EcoT22I 2477 ATGCAT ATGCA^T FauI 367 GCGGG CCCGC(4/6) Hin1I 170 GGCGTC G[GA]^CG[CT]C HincII 218 GTCAAC GT[CT]^[GA]AC HindII 218 GTCAAC GT[CT]^[GA]AC Ksp632I 674 GAAGAG CTCTTC(1/4) MfeI 3382 CAATTG C^AATTG MluNI 2451 TGGCCA TGG^CCA MscI 2451 TGGCCA TGG^CCA MunI 3382 CAATTG C^AATTG NheI 1703 GCTAGC G^CTAGC NsiI 2477 ATGCAT ATGCA^T NspV 1345 TTCGAA TT^CGAA PflFI 964 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC Psp1406I 3929 AACGTT AA^CGTT PstI 3170 CTGCAG CTGCA^G RcaI 147 TCATGA T^CATGA SacI 2373 GAGCTC GAGCT^C ScaI 2707 AGTACT AGT^ACT SfuI 1345 TTCGAA TT^CGAA Tth111I 964 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2379 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AocI 796 CCTGAGG CC^T[ACGT]AGG 3531 CCTAAGG BanII 478 GGGCCC G[GA]GC[CT]^C 2373 GAGCTC BcgI 2857 CGACACCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3193 CGAAGTCAGTGC BciVI 3058 GGATAC GTATCC(6/5) 1593 GTATCC BpmI 452 CTGGAG CTGGAG(16/14) 952 CTGGAG BsaBI 1457 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2004 GATCTTTATC BsaI 509 GAGACC GGTCTC(1/5) 962 GAGACC BsmI 1654 GAATGC GAATGC(1/-1) 3738 GCATTC Bsp1407I 325 TGTACA T^GTACA 383 TGTACA BsrGI 325 TGTACA T^GTACA 383 TGTACA Bsu36I 796 CCTGAGG CC^T[ACGT]AGG 3531 CCTAAGG CfoI 442 GCGC GCG^C 893 GCGC DraII 396 AGGACCT [GA]G^G[ACGT]CC[CT] 2983 GGGTCCC Eco81I 796 CCTGAGG CC^T[ACGT]AGG 3531 CCTAAGG EcoO109I 396 AGGACCT [GA]G^G[ACGT]CC[CT] 2983 GGGTCCC HaeII 441 GGCGCT [GA]GCGC^[CT] 892 AGCGCT HgaI 1399 GACGC GACGC(5/10) 171 GCGTC HhaI 442 GCGC GCG^C 893 GCGC HinP1I 442 GCGC G^CGC 893 GCGC MamI 1457 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2004 GATCTTTATC NcoI 538 CCATGG C^CATGG 2448 CCATGG PmeI 2883 GTTTAAAC GTTT^AAAC 3602 GTTTAAAC PpuMI 396 AGGACCT [GA]G^G[AT]CC[CT] 2983 GGGTCCC SfcI 635 CTACAG C^T[GA][CT]AG 3170 CTGCAG SphI 1715 GCATGC GCATG^C 2070 GCATGC SspBI 325 TGTACA T^GTACA 383 TGTACA TaqI 1341 TCGA T^CGA 1346 TCGA TthHB8I 1341 TCGA T^CGA 1346 TCGA XcmI 3023 CCAAAACACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3222 CCATTAATATCGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AcsI AfaI AflII AluI AlwI AlwNI ApoI AseI AsnI Asp700I AspHI AvaII BbsI BbvI BfaI BfrI BmyI BpuAI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DsaI EcoRII EcoT14I Fnu4HI FokI HaeIII HindIII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PshBI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII XmnI