##################################
# NO CUT ENZYMES (   68 enzymes) #
##################################

Acc65I      AccI        AclI        AflII       AgeI        
AocI        AscI        AseI        AsnI        Asp718I     
AspI        AvrII       BclI        BfaI        BfrI        
BlnI        BsaBI       BsiWI       Bsp1407I    BspDI       
BsrGI       BssHII      Bst1107I    BstBI       BstZ17I     
Bsu36I      ClaI        CpoI        DrdI        EagI        
EclXI       Eco52I      Eco81I      EcoT22I     FbaI        
FseI        KpnI        MaeI        MamI        MfeI        
MluI        MunI        NheI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SalI        SbfI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   25 enzymes) #
###################################

AatII        1671  GACGTC               GACGT^C
AhdI         3257  GACACATGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1865  GTGCAC               G^TGCAC
ApaLI        1865  GTGCAC               G^TGCAC
Asp700I       251  GAAGCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        3257  GACACATGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI         928  GGATCC               G^GATCC
BciVI        3446  GGATAC               GTATCC(6/5)
BsmI         3996  GCATTC               GAATGC(1/-1)
BspLU11I      412  ACATGT               A^CATGT
BstAPI       1577  GCAGAAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI         4163  TTTAAA               TTT^AAA
Eam1105I     3257  GACACATGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI        3153  GAATTC               G^AATTC
EcoRV         561  GATATC               GAT^ATC
HpaI         3857  GTTAAC               GTT^AAC
NcoI          136  CCATGG               C^CATGG
NdeI         4001  CATATG               CA^TATG
PaeR7I        588  CTCGAG               C^TCGAG
SacI         2900  GAGCTC               GAGCT^C
SapI         1763  GCTCTTC              GCTCTTC(1/4)
SfiI         3390  GGCCAGGAGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI         3187  GCATGC               GCATG^C
XhoI          588  CTCGAG               C^TCGAG
XmnI          251  GAAGCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AflIII        412  ACATGT               A^C[GA][CT]GT
             1525  ACGTGT              
AviII         616  TGCGCA               TGC^GCA
             1984  TGCGCA              
BbeI         1798  GGCGCC               GGCGC^C
             2251  GGCGCC              
BbrPI        2256  CACGTG               CAC^GTG
             4099  CACGTG              
BbvCI        2606  CCTCAGC              CCTCAGC(-5/-2)
             4083  GCTGAGG             
BlpI         1490  GCTGAGC              GC^T[ACGT]AGC
             3314  GCTGAGC             
Bpu1102I     1490  GCTGAGC              GC^T[ACGT]AGC
             3314  GCTGAGC             
BsmBI        1699  GAGACG               CGTCTC(1/5)
             2055  GAGACG              
BspHI         817  TCATGA               T^CATGA
             1507  TCATGA              
BsrDI         233  CATTGC               GCAATG(2/0)
             3001  GCAATG              
BstEII       2438  GGTCACC              G^GT[ACGT]ACC
             3586  GGTCACC             
BstPI        2438  GGTCACC              G^GT[ACGT]ACC
             3586  GGTCACC             
CelII        1490  GCTGAGC              GC^T[ACGT]AGC
             3314  GCTGAGC             
DraIII        171  CACAACGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1944  CACCAGGTG           
EcoO65I      2438  GGTCACC              G^GT[ACGT]ACC
             3586  GGTCACC             
FspI          616  TGCGCA               TGC^GCA
             1984  TGCGCA              
HincII       2115  GTCAAC               GT[CT]^[GA]AC
             3857  GTTAAC              
HindII       2115  GTCAAC               GT[CT]^[GA]AC
             3857  GTTAAC              
HindIII      2293  AAGCTT               A^AGCTT
             4015  AAGCTT              
KasI         1798  GGCGCC               G^GCGCC
             2251  GGCGCC              
KspI         2064  CCGCGG               CCGC^GG
             2822  CCGCGG              
NarI         1798  GGCGCC               GG^CGCC
             2251  GGCGCC              
PmaCI        2256  CACGTG               CAC^GTG
             4099  CACGTG              
PmlI         2256  CACGTG               CAC^GTG
             4099  CACGTG              
RcaI          817  TCATGA               T^CATGA
             1507  TCATGA              
SacII        2064  CCGCGG               CCGC^GG
             2822  CCGCGG              
ScaI          646  AGTACT               AGT^ACT
             1447  AGTACT              
SfoI         1798  GGCGCC               GGC^GCC
             2251  GGCGCC              


#########################################
# 3 OR MORE CUT ENZYMES (  137 enzymes) #
#########################################

AccII       AccIII      AciI        AcsI        AcyI        
AfaI        AluI        AlwI        AlwNI       Aor51HI     
ApaI        ApoI        AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbsI        BbvI        
BcgI        BcnI        BglI        BglII       BmyI        
BpmI        BpuAI       BsaAI       BsaHI       BsaI        
BsaJI       BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspEI       BspMI       
BsrBI       BsrFI       BsrI        BssKI       BssSI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
Eco47III    EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MroI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NciI        NdeII       NgoMIV      
NlaIII      NlaIV       NspI        PflMI       PleI        
PpuMI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SexAI       SfaNI       SfcI        
SmaI        SmlI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII       
XmaCI       XmaI