################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## Acc65I AccI AclI AflII AgeI AocI AscI AseI AsnI Asp718I AspI AvrII BclI BfaI BfrI BlnI BsaBI BsiWI Bsp1407I BspDI BsrGI BssHII Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DrdI EagI EclXI Eco52I Eco81I EcoT22I FbaI FseI KpnI MaeI MamI MfeI MluI MunI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI SbfI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 24 enzymes) # ################################### AatII 1802 GACGTC GACGT^C AhdI 3388 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1996 GTGCAC G^TGCAC ApaLI 1996 GTGCAC G^TGCAC Asp700I 382 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 3388 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 1059 GGATCC G^GATCC BciVI 3577 GGATAC GTATCC(6/5) BsmI 4127 GCATTC GAATGC(1/-1) BspLU11I 543 ACATGT A^CATGT BstAPI 1708 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraI 4294 TTTAAA TTT^AAA Eam1105I 3388 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 3284 GAATTC G^AATTC EcoRV 692 GATATC GAT^ATC HpaI 3988 GTTAAC GTT^AAC NdeI 4132 CATATG CA^TATG PaeR7I 719 CTCGAG C^TCGAG SacI 3031 GAGCTC GAGCT^C SapI 1894 GCTCTTC GCTCTTC(1/4) SfiI 3521 GGCCAGGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 3318 GCATGC GCATG^C XhoI 719 CTCGAG C^TCGAG XmnI 382 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AflIII 543 ACATGT A^C[GA][CT]GT 1656 ACGTGT AviII 747 TGCGCA TGC^GCA 2115 TGCGCA BbeI 1929 GGCGCC GGCGC^C 2382 GGCGCC BbrPI 2387 CACGTG CAC^GTG 4230 CACGTG BbvCI 2737 CCTCAGC CCTCAGC(-5/-2) 4214 GCTGAGG BlpI 1621 GCTGAGC GC^T[ACGT]AGC 3445 GCTGAGC Bpu1102I 1621 GCTGAGC GC^T[ACGT]AGC 3445 GCTGAGC BsmBI 1830 GAGACG CGTCTC(1/5) 2186 GAGACG BspHI 948 TCATGA T^CATGA 1638 TCATGA BsrDI 364 CATTGC GCAATG(2/0) 3132 GCAATG BstEII 2569 GGTCACC G^GT[ACGT]ACC 3717 GGTCACC BstPI 2569 GGTCACC G^GT[ACGT]ACC 3717 GGTCACC CelII 1621 GCTGAGC GC^T[ACGT]AGC 3445 GCTGAGC DraIII 302 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG 2075 CACCAGGTG EcoO65I 2569 GGTCACC G^GT[ACGT]ACC 3717 GGTCACC FspI 747 TGCGCA TGC^GCA 2115 TGCGCA HincII 2246 GTCAAC GT[CT]^[GA]AC 3988 GTTAAC HindII 2246 GTCAAC GT[CT]^[GA]AC 3988 GTTAAC HindIII 2424 AAGCTT A^AGCTT 4146 AAGCTT KasI 1929 GGCGCC G^GCGCC 2382 GGCGCC KspI 2195 CCGCGG CCGC^GG 2953 CCGCGG NarI 1929 GGCGCC GG^CGCC 2382 GGCGCC NcoI 82 CCATGG C^CATGG 267 CCATGG PmaCI 2387 CACGTG CAC^GTG 4230 CACGTG PmlI 2387 CACGTG CAC^GTG 4230 CACGTG RcaI 948 TCATGA T^CATGA 1638 TCATGA SacII 2195 CCGCGG CCGC^GG 2953 CCGCGG ScaI 777 AGTACT AGT^ACT 1578 AGTACT SfoI 1929 GGCGCC GGC^GCC 2382 GGCGCC ######################################### # 3 OR MORE CUT ENZYMES ( 137 enzymes) # ######################################### AccII AccIII AciI AcsI AcyI AfaI AluI AlwI AlwNI Aor51HI ApaI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcgI BcnI BglI BglII BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco47III EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI