##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
AocI AscI AseI AsnI Asp718I
AspI AvrII BclI BfaI BfrI
BlnI BsaBI BsiWI Bsp1407I BspDI
BsrGI BssHII Bst1107I BstBI BstZ17I
Bsu36I ClaI CpoI DrdI EagI
EclXI Eco52I Eco81I EcoT22I FbaI
FseI KpnI MaeI MamI MfeI
MluI MunI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI SbfI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 24 enzymes) #
###################################
AatII 1802 GACGTC GACGT^C
AhdI 3388 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1996 GTGCAC G^TGCAC
ApaLI 1996 GTGCAC G^TGCAC
Asp700I 382 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3388 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1059 GGATCC G^GATCC
BciVI 3577 GGATAC GTATCC(6/5)
BsmI 4127 GCATTC GAATGC(1/-1)
BspLU11I 543 ACATGT A^CATGT
BstAPI 1708 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 4294 TTTAAA TTT^AAA
Eam1105I 3388 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3284 GAATTC G^AATTC
EcoRV 692 GATATC GAT^ATC
HpaI 3988 GTTAAC GTT^AAC
NdeI 4132 CATATG CA^TATG
PaeR7I 719 CTCGAG C^TCGAG
SacI 3031 GAGCTC GAGCT^C
SapI 1894 GCTCTTC GCTCTTC(1/4)
SfiI 3521 GGCCAGGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 3318 GCATGC GCATG^C
XhoI 719 CTCGAG C^TCGAG
XmnI 382 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 543 ACATGT A^C[GA][CT]GT
1656 ACGTGT
AviII 747 TGCGCA TGC^GCA
2115 TGCGCA
BbeI 1929 GGCGCC GGCGC^C
2382 GGCGCC
BbrPI 2387 CACGTG CAC^GTG
4230 CACGTG
BbvCI 2737 CCTCAGC CCTCAGC(-5/-2)
4214 GCTGAGG
BlpI 1621 GCTGAGC GC^T[ACGT]AGC
3445 GCTGAGC
Bpu1102I 1621 GCTGAGC GC^T[ACGT]AGC
3445 GCTGAGC
BsmBI 1830 GAGACG CGTCTC(1/5)
2186 GAGACG
BspHI 948 TCATGA T^CATGA
1638 TCATGA
BsrDI 364 CATTGC GCAATG(2/0)
3132 GCAATG
BstEII 2569 GGTCACC G^GT[ACGT]ACC
3717 GGTCACC
BstPI 2569 GGTCACC G^GT[ACGT]ACC
3717 GGTCACC
CelII 1621 GCTGAGC GC^T[ACGT]AGC
3445 GCTGAGC
DraIII 302 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
2075 CACCAGGTG
EcoO65I 2569 GGTCACC G^GT[ACGT]ACC
3717 GGTCACC
FspI 747 TGCGCA TGC^GCA
2115 TGCGCA
HincII 2246 GTCAAC GT[CT]^[GA]AC
3988 GTTAAC
HindII 2246 GTCAAC GT[CT]^[GA]AC
3988 GTTAAC
HindIII 2424 AAGCTT A^AGCTT
4146 AAGCTT
KasI 1929 GGCGCC G^GCGCC
2382 GGCGCC
KspI 2195 CCGCGG CCGC^GG
2953 CCGCGG
NarI 1929 GGCGCC GG^CGCC
2382 GGCGCC
NcoI 82 CCATGG C^CATGG
267 CCATGG
PmaCI 2387 CACGTG CAC^GTG
4230 CACGTG
PmlI 2387 CACGTG CAC^GTG
4230 CACGTG
RcaI 948 TCATGA T^CATGA
1638 TCATGA
SacII 2195 CCGCGG CCGC^GG
2953 CCGCGG
ScaI 777 AGTACT AGT^ACT
1578 AGTACT
SfoI 1929 GGCGCC GGC^GCC
2382 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AluI AlwI AlwNI Aor51HI
ApaI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcgI BcnI BglI BglII BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspEI BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco47III EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI