##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflIII AgeI Aor51HI AscI AseI
AsnI Asp718I AspI AviII AvrII
BamHI BanI BbeI BbrPI BglI
BlnI BsaAI BseAI BsiEI BsiWI
BspEI BspHI BspLU11I BssHII Bst1107I
BstBI BstZ17I CpoI EagI EclXI
Eco47III Eco52I EcoRI EcoT22I FseI
FspI HpaI KasI KpnI MluI
MroI NarI NdeI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SwaI
Tth111I XcmI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AcyI 2674 GACGCC G[GA]^CG[CT]C
AflII 4168 CTTAAG C^TTAAG
AhdI 3802 GACTGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 929 GTGCAC G^TGCAC
ApaLI 929 GTGCAC G^TGCAC
AspEI 3802 GACTGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 2193 CCTCAGC CCTCAGC(-5/-2)
BfrI 4168 CTTAAG C^TTAAG
BglII 1078 AGATCT A^GATCT
BlpI 3610 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3610 GCTAAGC GC^T[ACGT]AGC
BsaHI 2674 GACGCC G[GA]^CG[CT]C
BsmI 1317 GCATTC GAATGC(1/-1)
BspDI 3763 ATCGAT AT^CGAT
BspMI 3436 GCAGGT ACCTGC(4/8)
BsrFI 8 GCCGGC [GA]^CCGG[CT]
BstAPI 4028 GCATCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3413 GGTTACC G^GT[ACGT]ACC
BstPI 3413 GGTTACC G^GT[ACGT]ACC
CelII 3610 GCTAAGC GC^T[ACGT]AGC
Cfr10I 8 GCCGGC [GA]^CCGG[CT]
ClaI 3763 ATCGAT AT^CGAT
DraI 3876 TTTAAA TTT^AAA
DraIII 2361 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3802 GACTGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1849 CCTGGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3413 GGTTACC G^GT[ACGT]ACC
Hin1I 2674 GACGCC G[GA]^CG[CT]C
HincII 1574 GTTGAC GT[CT]^[GA]AC
HindII 1574 GTTGAC GT[CT]^[GA]AC
HindIII 495 AAGCTT A^AGCTT
KspI 99 CCGCGG CCGC^GG
MfeI 1763 CAATTG C^AATTG
MunI 1763 CAATTG C^AATTG
NaeI 8 GCCGGC GCC^GGC
NgoMIV 8 GCCGGC G^CCGGC
SacII 99 CCGCGG CCGC^GG
SexAI 2362 ACCAGGT A^CC[AT]GGT
SmaI 681 CCCGGG CCC^GGG
SphI 1666 GCATGC GCATG^C
SspI 4156 AATATT AAT^ATT
StuI 922 AGGCCT AGG^CCT
XbaI 704 TCTAGA T^CTAGA
XmaCI 681 CCCGGG C^CCGGG
XmaI 681 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 100 CGCG CG^CG
2083 CGCG
AocI 1325 CCTAAGG CC^T[ACGT]AGG
3780 CCTGAGG
Asp700I 1286 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3091 GAACGTCTTC
BbsI 2378 GTCTTC GAAGAC(2/6)
3095 GTCTTC
BcgI 1910 GCAGCTATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2570 GCAGAGACATCG
BciVI 3785 GGATAC GTATCC(6/5)
3071 GTATCC
BclI 204 TGATCA T^GATCA
2907 TGATCA
BpuAI 2378 GTCTTC GAAGAC(2/6)
3095 GTCTTC
BsaBI 1055 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1711 GATGTCAATC
BsmBI 305 CGTCTC CGTCTC(1/5)
4119 CGTCTC
BsrBI 658 CCGCTC CCGCTC(-3/-3)
773 GAGCGG
BsrDI 3963 CATTGC GCAATG(2/0)
1472 GCAATG
BssSI 2666 CTCGTG CACGAG(-5/-1)
4148 CTCGTG
BstUI 100 CGCG CG^CG
2083 CGCG
Bsu36I 1325 CCTAAGG CC^T[ACGT]AGG
3780 CCTGAGG
DrdI 1705 GACCCAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3769 GACAGGCTGGTC
Eco81I 1325 CCTAAGG CC^T[ACGT]AGG
3780 CCTGAGG
EcoRV 708 GATATC GAT^ATC
2369 GATATC
FbaI 204 TGATCA T^GATCA
2907 TGATCA
MamI 1055 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1711 GATGTCAATC
MvnI 100 CGCG CG^CG
2083 CGCG
NheI 3181 GCTAGC G^CTAGC
4068 GCTAGC
PaeR7I 119 CTCGAG C^TCGAG
3687 CTCGAG
SacI 941 GAGCTC GAGCT^C
3939 GAGCTC
XhoI 119 CTCGAG C^TCGAG
3687 CTCGAG
XmnI 1286 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3091 GAACGTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApaI ApoI AspHI AvaI
AvaII BalI BanII BbvI BcnI
BfaI BmyI BpmI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BsrGI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII