##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AclI AflII AgeI AhdI AocI
AscI AseI AsnI AspEI AviII
BbeI BbrPI BciVI BclI BfrI
BglII BsiWI BsmBI BsmI BspDI
BspHI BssHII Bst1107I BstBI BstZ17I
Bsu36I ClaI CpoI Eam1105I Eco81I
EcoRI EcoRV FbaI FseI FspI
HindIII KasI KspI MluI NarI
NcoI NdeI NotI NspV PacI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII ScaI SexAI SfoI SfuI
SgrAI SnaBI SpeI SphI SspI
SwaI Van91I XbaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 3601 GACGTC GACGT^C
AccIII 4088 TCCGGA T^CCGGA
AflIII 2420 ACATGT A^C[GA][CT]GT
ApaI 1763 GGGCCC GGGCC^C
AspI 3465 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 4081 GGATCC G^GATCC
BcgI 2777 GCATGATCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 2164 TACGTG [CT]AC^GT[GA]
BsaBI 2271 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4088 TCCGGA [AT]^CCGG[AT]
BseAI 4088 TCCGGA T^CCGGA
BspEI 4088 TCCGGA T^CCGGA
BspLU11I 2420 ACATGT A^CATGT
BstAPI 2844 GCACCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 4081 GGATCC [GA]^GATC[CT]
DraI 1943 TTTAAA TTT^AAA
EagI 1347 CGGCCG C^GGCCG
EclXI 1347 CGGCCG C^GGCCG
Eco52I 1347 CGGCCG C^GGCCG
EcoT22I 1306 ATGCAT ATGCA^T
HpaI 1828 GTTAAC GTT^AAC
MamI 2271 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3861 CAATTG C^AATTG
MflI 4081 GGATCC [GA]^GATC[CT]
MroI 4088 TCCGGA T^CCGGA
MunI 3861 CAATTG C^AATTG
NaeI 3036 GCCGGC GCC^GGC
NgoMIV 3036 GCCGGC G^CCGGC
NruI 3257 TCGCGA TCG^CGA
NsiI 1306 ATGCAT ATGCA^T
PaeR7I 2650 CTCGAG C^TCGAG
PflFI 3465 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1425 GACTCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 3471 GTCGAC G^TCGAC
SapI 2403 GCTCTTC GCTCTTC(1/4)
SfiI 3138 GGCCGCATAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 2650 CTCGAG C^T[CT][GA]AG
Tth111I 3465 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2213 CCATCGGCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2650 CTCGAG C^TCGAG
XhoII 4081 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 1149 GGTACC G^GTACC
1679 GGTACC
AccI 3126 GTAGAC GT^[AC][GT]AC
3471 GTCGAC
AcyI 1738 GACGCC G[GA]^CG[CT]C
3601 GACGTC
Aor51HI 3354 AGCGCT AGC^GCT
3767 AGCGCT
Asp718I 1149 GGTACC G^GTACC
1679 GGTACC
AvrII 502 CCTAGG C^CTAGG
1014 CCTAGG
BalI 2126 TGGCCA TGG^CCA
2477 TGGCCA
BlnI 502 CCTAGG C^CTAGG
1014 CCTAGG
BsaHI 1738 GACGCC G[GA]^CG[CT]C
3601 GACGTC
BsaI 599 GAGACC GGTCTC(1/5)
2986 GAGACC
BsiEI 1347 CGGCCG CG[GA][CT]^CG
2460 CGACCG
Bsp1407I 2423 TGTACA T^GTACA
3011 TGTACA
BsrGI 2423 TGTACA T^GTACA
3011 TGTACA
BstXI 104 CCAGCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1227 CCAGCTTTCTGG
DraIII 743 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
889 CACGGGGTG
DrdI 2374 GACATCGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3388 GACCAGCCTGTC
Eco47III 3354 AGCGCT AGC^GCT
3767 AGCGCT
Hin1I 1738 GACGCC G[GA]^CG[CT]C
3601 GACGTC
KpnI 1149 GGTACC GGTAC^C
1679 GGTACC
MluNI 2126 TGGCCA TGG^CCA
2477 TGGCCA
MscI 2126 TGGCCA TGG^CCA
2477 TGGCCA
NheI 3636 GCTAGC G^CTAGC
3640 GCTAGC
NspI 2420 ACATGT [GA]CATG^[CT]
3675 ACATGC
SbfI 1467 CCTGCAGG CCTGCA^GG
2409 CCTGCAGG
Sse8387I 1467 CCTGCAGG CCTGCA^GG
2409 CCTGCAGG
SspBI 2423 TGTACA T^GTACA
3011 TGTACA
StuI 1618 AGGCCT AGG^CCT
2609 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
Alw44I AlwI AlwNI ApaLI ApoI
Asp700I AspHI AvaI AvaII BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BssSI BstEII
BstF5I BstNI BstPI BstUI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XmaCI
XmaI XmnI