##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
Acc65I AgeI AhdI AocI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BbrPI BbvCI BcgI
BglI BlnI BlpI Bpu1102I BsaBI
BsiWI BspDI BspHI BstAPI BstBI
Bsu36I CelII ClaI CpoI DraIII
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoT14I EcoT22I FseI FspI
KpnI Ksp632I MamI MfeI MunI
NcoI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI PvuI
RcaI RsrII SalI SapI SbfI
ScaI SexAI SfiI SfuI SnaBI
SpeI Sse8387I StuI StyI Tth111I
XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 3612 GACGTC GACGT^C
AccIII 715 TCCGGA T^CCGGA
AclI 860 AACGTT AA^CGTT
AflII 991 CTTAAG C^TTAAG
AlwNI 248 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 2161 GGGCCC GGGCC^C
Asp700I 514 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 3282 GGATCC G^GATCC
BciVI 2624 GTATCC GTATCC(6/5)
BfrI 991 CTTAAG C^TTAAG
BglII 3697 AGATCT A^GATCT
BsaAI 1619 TACGTG [CT]AC^GT[GA]
BseAI 715 TCCGGA T^CCGGA
BsgI 403 GTGCAG GTGCAG(16/14)
BsmBI 3403 GAGACG CGTCTC(1/5)
BsmI 2577 GCATTC GAATGC(1/-1)
BspEI 715 TCCGGA T^CCGGA
BspLU11I 3717 ACATGT A^CATGT
BssSI 1673 CACGAG CACGAG(-5/-1)
Bst1107I 838 GTATAC GTA^TAC
BstXI 2088 CCACTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 838 GTATAC GTA^TAC
DrdI 3590 GACCCTAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 581 GATATC GAT^ATC
HpaI 3147 GTTAAC GTT^AAC
MroI 715 TCCGGA T^CCGGA
NheI 4267 GCTAGC G^CTAGC
PflMI 3390 CCAGATATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 860 AACGTT AA^CGTT
PvuII 2695 CAGCTG CAG^CTG
SacI 1332 GAGCTC GAGCT^C
SmaI 2419 CCCGGG CCC^GGG
SphI 2544 GCATGC GCATG^C
SspI 4202 AATATT AAT^ATT
SwaI 3954 ATTTAAAT ATTT^AAAT
Van91I 3390 CCAGATATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3097 TCTAGA T^CTAGA
XmaCI 2419 CCCGGG C^CCGGG
XmaI 2419 CCCGGG C^CCGGG
XmnI 514 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Aor51HI 1602 AGCGCT AGC^GCT
2071 AGCGCT
BalI 2615 TGGCCA TGG^CCA
3350 TGGCCA
BbeI 1856 GGCGCC GGCGC^C
2122 GGCGCC
BbsI 83 GAAGAC GAAGAC(2/6)
2395 GAAGAC
BpuAI 83 GAAGAC GAAGAC(2/6)
2395 GAAGAC
BsaWI 715 TCCGGA [AT]^CCGG[AT]
2735 TCCGGT
BsiEI 370 CGACCG CG[GA][CT]^CG
1847 CGGTCG
BsrDI 3965 CATTGC GCAATG(2/0)
164 GCAATG
BsrFI 1786 GCCGGC [GA]^CCGG[CT]
1859 GCCGGC
BstEII 1252 GGTGACC G^GT[ACGT]ACC
1372 GGTTACC
BstPI 1252 GGTGACC G^GT[ACGT]ACC
1372 GGTTACC
Cfr10I 1786 GCCGGC [GA]^CCGG[CT]
1859 GCCGGC
Eco47III 1602 AGCGCT AGC^GCT
2071 AGCGCT
EcoNI 319 CCTTCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2563 CCTAGAGGAGG
EcoO65I 1252 GGTGACC G^GT[ACGT]ACC
1372 GGTTACC
EcoRI 358 GAATTC G^AATTC
3937 GAATTC
HindIII 3128 AAGCTT A^AGCTT
4464 AAGCTT
KasI 1856 GGCGCC G^GCGCC
2122 GGCGCC
KspI 310 CCGCGG CCGC^GG
3373 CCGCGG
MluI 1911 ACGCGT A^CGCGT
2000 ACGCGT
MluNI 2615 TGGCCA TGG^CCA
3350 TGGCCA
MscI 2615 TGGCCA TGG^CCA
3350 TGGCCA
NaeI 1786 GCCGGC GCC^GGC
1859 GCCGGC
NarI 1856 GGCGCC GG^CGCC
2122 GGCGCC
NdeI 1019 CATATG CA^TATG
4366 CATATG
NgoMIV 1786 GCCGGC G^CCGGC
1859 GCCGGC
SacII 310 CCGCGG CCGC^GG
3373 CCGCGG
SfoI 1856 GGCGCC GGC^GCC
2122 GGCGCC
SgrAI 1785 CGCCGGCG C[GA]^CCGG[CT]G
1858 CGCCGGCG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
ApaLI ApoI AspHI AvaI AvaII
BanI BanII BbvI BclI BcnI
BfaI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrBI BsrGI BsrI BssHII
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EcoO109I
EcoRII FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII