##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AccIII AhdI Alw44I ApaLI AscI
AspEI AspI AviII AvrII BbeI
BbrPI BcgI BlnI BsaAI BsaBI
BseAI BsiEI BsiWI Bsp1407I BspEI
BsrBI BsrGI BssHII BssSI BstAPI
BstBI CpoI EagI Eam1105I EclXI
Eco52I EcoNI FseI FspI HpaI
KasI KspI MamI MfeI MluI
MroI MunI NaeI NarI NgoMIV
NotI NruI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PvuI
RsrII SacII SalI SbfI SexAI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I SspBI StuI SwaI Tth111I
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 1942 GACGTC GACGT^C
Acc65I 2382 GGTACC G^GTACC
AccII 6 CGCG CG^CG
AclI 1096 AACGTT AA^CGTT
AflII 170 CTTAAG C^TTAAG
AgeI 306 ACCGGT A^CCGGT
AocI 664 CCTCAGG CC^T[ACGT]AGG
ApaI 1562 GGGCCC GGGCC^C
Asp718I 2382 GGTACC G^GTACC
BalI 4222 TGGCCA TGG^CCA
BamHI 527 GGATCC G^GATCC
BciVI 3120 GTATCC GTATCC(6/5)
BfrI 170 CTTAAG C^TTAAG
BglI 1760 GCCAGACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3170 AGATCT A^GATCT
BsaWI 306 ACCGGT [AT]^CCGG[AT]
BspDI 2892 ATCGAT AT^CGAT
BspHI 3595 TCATGA T^CATGA
BspMI 4138 GCAGGT ACCTGC(4/8)
BsrDI 280 CATTGC GCAATG(2/0)
BstEII 336 GGTGACC G^GT[ACGT]ACC
BstPI 336 GGTGACC G^GT[ACGT]ACC
BstUI 6 CGCG CG^CG
Bsu36I 664 CCTCAGG CC^T[ACGT]AGG
ClaI 2892 ATCGAT AT^CGAT
Eco81I 664 CCTCAGG CC^T[ACGT]AGG
EcoO65I 336 GGTGACC G^GT[ACGT]ACC
EcoRV 1309 GATATC GAT^ATC
KpnI 2382 GGTACC GGTAC^C
MluNI 4222 TGGCCA TGG^CCA
MscI 4222 TGGCCA TGG^CCA
MvnI 6 CGCG CG^CG
NcoI 2749 CCATGG C^CATGG
NdeI 1639 CATATG CA^TATG
NheI 2316 GCTAGC G^CTAGC
PinAI 306 ACCGGT A^CCGGT
PpuMI 432 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 3366 GACCCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1096 AACGTT AA^CGTT
RcaI 3595 TCATGA T^CATGA
SfiI 1759 GGCCAGACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 3758 ACTAGT A^CTAGT
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 1942 GACGTC G[GA]^CG[CT]C
2884 GGCGTC
Aor51HI 77 AGCGCT AGC^GCT
1507 AGCGCT
AvaI 1782 CTCGGG C^[CT]CG[GA]G
3144 CCCGAG
BanI 2382 GGTACC G^G[CT][GA]CC
2541 GGTGCC
BclI 2393 TGATCA T^GATCA
3679 TGATCA
BcnI 1967 CCGGG CC^[GC]GG
2920 CCGGG
BsaHI 1942 GACGTC G[GA]^CG[CT]C
2884 GGCGTC
BsaI 309 GGTCTC GGTCTC(1/5)
698 GGTCTC
BsmBI 149 CGTCTC CGTCTC(1/5)
4296 GAGACG
BsoBI 1782 CTCGGG C^[CT]CG[GA]G
3144 CCCGAG
BsrFI 306 ACCGGT [GA]^CCGG[CT]
1990 GCCGGT
Bst1107I 2338 GTATAC GTA^TAC
3914 GTATAC
BstZ17I 2338 GTATAC GTA^TAC
3914 GTATAC
Cfr10I 306 ACCGGT [GA]^CCGG[CT]
1990 GCCGGT
DraI 1092 TTTAAA TTT^AAA
4246 TTTAAA
DraIII 2812 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
3626 CACTGAGTG
DrdI 753 GACTTTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3177 GACAGACTGGTC
DsaI 2749 CCATGG C^C[GA][CT]GG
2771 CCGTGG
EaeI 1406 TGGCCG [CT]^GGCC[GA]
4222 TGGCCA
Eco47III 77 AGCGCT AGC^GCT
1507 AGCGCT
EcoRI 475 GAATTC G^AATTC
3721 GAATTC
FbaI 2393 TGATCA T^GATCA
3679 TGATCA
HaeII 77 AGCGCT [GA]GCGC^[CT]
1507 AGCGCT
Hin1I 1942 GACGTC G[GA]^CG[CT]C
2884 GGCGTC
HincII 275 GTCAAC GT[CT]^[GA]AC
2244 GTCAAC
HindII 275 GTCAAC GT[CT]^[GA]AC
2244 GTCAAC
HindIII 1711 AAGCTT A^AGCTT
3449 AAGCTT
NciI 1967 CCGGG CC^[GC]GG
2920 CCGGG
PflMI 437 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2744 CCATGCCATGG
PstI 1908 CTGCAG CTGCA^G
2847 CTGCAG
ScaI 1286 AGTACT AGT^ACT
1327 AGTACT
Van91I 437 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2744 CCATGCCATGG
XbaI 839 TCTAGA T^CTAGA
3173 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AspHI AvaII BanII
BbsI BbvCI BbvI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspLU11I
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NsiI NspI PleI
PshBI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI