##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AcyI AflIII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AviII AvrII BbeI
BbrPI BciVI BclI BglII BlnI
BsaAI BsaHI BsiEI BsiWI BsmI
BspDI BspHI BspLU11I BssHII BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoNI EcoRI EcoRV FbaI FseI
FspI Hin1I KasI KpnI KspI
MluI NarI NdeI NotI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI RcaI
RsrII SacII SalI SexAI SfiI
SfoI SfuI SgrAI SnaBI SspI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 3339 GTAGAC GT^[AC][GT]AC
AclI 1419 AACGTT AA^CGTT
AflII 2330 CTTAAG C^TTAAG
AocI 1009 CCTGAGG CC^T[ACGT]AGG
BalI 2668 TGGCCA TGG^CCA
BbvCI 3647 CCTCAGC CCTCAGC(-5/-2)
BcgI 2091 CGAAGGAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2330 CTTAAG C^TTAAG
BsaBI 2826 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 2695 CGTCTC CGTCTC(1/5)
Bsp1407I 3926 TGTACA T^GTACA
BspMI 2027 ACCTGC ACCTGC(4/8)
BsrBI 31 CCGCTC CCGCTC(-3/-3)
BsrFI 1956 GCCGGC [GA]^CCGG[CT]
BsrGI 3926 TGTACA T^GTACA
Bsu36I 1009 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1956 GCCGGC [GA]^CCGG[CT]
DraIII 1458 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 4197 CTCTTC CTCTTC(1/4)
Eco81I 1009 CCTGAGG CC^T[ACGT]AGG
EcoT22I 2497 ATGCAT ATGCA^T
HaeII 1953 AGCGCC [GA]GCGC^[CT]
HpaI 2860 GTTAAC GTT^AAC
Ksp632I 4197 CTCTTC CTCTTC(1/4)
MamI 2826 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2668 TGGCCA TGG^CCA
MscI 2668 TGGCCA TGG^CCA
NaeI 1956 GCCGGC GCC^GGC
NcoI 3671 CCATGG C^CATGG
NgoMIV 1956 GCCGGC G^CCGGC
NheI 3664 GCTAGC G^CTAGC
NruI 143 TCGCGA TCG^CGA
NsiI 2497 ATGCAT ATGCA^T
Psp1406I 1419 AACGTT AA^CGTT
SapI 4196 GCTCTTC GCTCTTC(1/4)
SbfI 3265 CCTGCAGG CCTGCA^GG
ScaI 294 AGTACT AGT^ACT
SmaI 2202 CCCGGG CCC^GGG
SpeI 3453 ACTAGT A^CTAGT
Sse8387I 3265 CCTGCAGG CCTGCA^GG
SspBI 3926 TGTACA T^GTACA
XmaCI 2202 CCCGGG C^CCGGG
XmaI 2202 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 35 TCCGGA T^CCGGA
1314 TCCGGA
AhdI 134 GACGGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
425 GACCCAGAGTC
ApaI 567 GGGCCC GGGCC^C
1684 GGGCCC
Asp700I 3086 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3251 GAATTTCTTC
AspEI 134 GACGGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
425 GACCCAGAGTC
AspI 1972 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3982 GACAAAGTC
BanI 1982 GGCACC G^G[CT][GA]CC
2377 GGTGCC
BglI 191 GCCCAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1833 GCCCTCCTGGC
BsaWI 35 TCCGGA [AT]^CCGG[AT]
1314 TCCGGA
BseAI 35 TCCGGA T^CCGGA
1314 TCCGGA
BspEI 35 TCCGGA T^CCGGA
1314 TCCGGA
BstAPI 448 GCACAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2022 GCATTACCTGC
BstEII 1397 GGTTACC G^GT[ACGT]ACC
2651 GGTGACC
BstPI 1397 GGTTACC G^GT[ACGT]ACC
2651 GGTGACC
DraI 3550 TTTAAA TTT^AAA
3705 TTTAAA
Eam1105I 134 GACGGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
425 GACCCAGAGTC
EcoO65I 1397 GGTTACC G^GT[ACGT]ACC
2651 GGTGACC
HgaI 1920 GACGC GACGC(5/10)
1123 GCGTC
HincII 2860 GTTAAC GT[CT]^[GA]AC
3038 GTTGAC
HindII 2860 GTTAAC GT[CT]^[GA]AC
3038 GTTGAC
HindIII 2211 AAGCTT A^AGCTT
3442 AAGCTT
MaeII 1420 ACGT A^CGT
2694 ACGT
MfeI 572 CAATTG C^AATTG
1584 CAATTG
MroI 35 TCCGGA T^CCGGA
1314 TCCGGA
MslI 2045 CACCAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2892 CATTGGGGTG
MunI 572 CAATTG C^AATTG
1584 CAATTG
PflFI 1972 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3982 GACAAAGTC
PflMI 379 CCACTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
952 CCACGGGTTGG
StuI 1726 AGGCCT AGG^CCT
2877 AGGCCT
Tth111I 1972 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3982 GACAAAGTC
Van91I 379 CCACTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
952 CCACGGGTTGG
XmnI 3086 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3251 GAATTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BamHI BanII BbsI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII