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# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AclI AgeI Aor51HI AscI
Asp700I AspI AvrII BamHI BbrPI
BbvCI BcgI BclI BlnI BlpI
Bpu1102I BsiWI BsmBI BsmI BspDI
BssSI BstBI CelII ClaI Eco47III
EcoRV EcoT22I FbaI FseI HindIII
HpaI MfeI MluI MunI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI Psp1406I PvuI SalI SapI
SfiI SfuI SnaBI SspI StuI
SwaI Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccIII 122 TCCGGA T^CCGGA
AcsI 1383 GAATTC [GA]^AATT[CT]
AflII 1778 CTTAAG C^TTAAG
AflIII 3932 ACATGT A^C[GA][CT]GT
AhdI 2586 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2037 GTGCAC G^TGCAC
ApaLI 2037 GTGCAC G^TGCAC
ApoI 1383 GAATTC [GA]^AATT[CT]
AseI 3762 ATTAAT AT^TAAT
AsnI 3762 ATTAAT AT^TAAT
AspEI 2586 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2065 TGCGCA TGC^GCA
BalI 2047 TGGCCA TGG^CCA
BciVI 678 GTATCC GTATCC(6/5)
BfrI 1778 CTTAAG C^TTAAG
BsaBI 1925 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 122 TCCGGA T^CCGGA
BspEI 122 TCCGGA T^CCGGA
BspHI 3182 TCATGA T^CATGA
BspLU11I 3932 ACATGT A^CATGT
BsrDI 151 CATTGC GCAATG(2/0)
Bst1107I 1811 GTATAC GTA^TAC
BstZ17I 1811 GTATAC GTA^TAC
CpoI 227 CGGTCCG CG^G[AT]CCG
DraIII 3902 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2586 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1383 GAATTC G^AATTC
FspI 2065 TGCGCA TGC^GCA
HincII 1862 GTTGAC GT[CT]^[GA]AC
HindII 1862 GTTGAC GT[CT]^[GA]AC
MamI 1925 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2047 TGGCCA TGG^CCA
MroI 122 TCCGGA T^CCGGA
MscI 2047 TGGCCA TGG^CCA
NcoI 682 CCATGG C^CATGG
PshAI 2071 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3762 ATTAAT AT^TAAT
RcaI 3182 TCATGA T^CATGA
RsrII 227 CGGTCCG CG^G[AT]CCG
SbfI 2507 CCTGCAGG CCTGCA^GG
ScaI 3115 AGTACT AGT^ACT
SgrAI 63 CGCCGGTG C[GA]^CCGG[CT]G
SmlI 1778 CTTAAG C^T[CT][GA]AG
SpeI 3774 ACTAGT A^CTAGT
Sse8387I 2507 CCTGCAGG CCTGCA^GG
TfiI 3168 GATTC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccI 1811 GTATAC GT^[AC][GT]AC
1983 GTCTAC
AocI 3157 CCTCAGG CC^T[ACGT]AGG
3676 CCTCAGG
ApaI 3325 GGGCCC GGGCC^C
3856 GGGCCC
BglII 1723 AGATCT A^GATCT
2866 AGATCT
BsaAI 1814 TACGTG [CT]AC^GT[GA]
3149 TACGTG
Bsp1407I 1633 TGTACA T^GTACA
2977 TGTACA
BsrBI 586 CCGCTC CCGCTC(-3/-3)
1542 GAGCGG
BsrGI 1633 TGTACA T^GTACA
2977 TGTACA
BssHII 79 GCGCGC G^CGCGC
183 GCGCGC
Bsu36I 3157 CCTCAGG CC^T[ACGT]AGG
3676 CCTCAGG
DrdI 2217 GACAGACTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3284 GACTCCACCGTC
Eco81I 3157 CCTCAGG CC^T[ACGT]AGG
3676 CCTCAGG
EcoNI 811 CCTGAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2240 CCTGGGAGAGG
NheI 46 GCTAGC G^CTAGC
2159 GCTAGC
SexAI 2587 ACCTGGT A^CC[AT]GGT
2605 ACCAGGT
SspBI 1633 TGTACA T^GTACA
2977 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AluI AlwI AlwNI Asp718I AspHI
AvaI AvaII BanI BanII BbeI
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EagI
EarI EclXI Eco52I EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI KpnI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SphI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI