################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## AatII AclI AgeI Aor51HI AscI Asp700I AspI AvrII BamHI BbrPI BbvCI BcgI BclI BlnI BlpI Bpu1102I BsiWI BsmBI BsmI BspDI BssSI BstBI CelII ClaI Eco47III EcoRV EcoT22I FbaI FseI HindIII HpaI MfeI MluI MunI NdeI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI SalI SapI SfiI SfuI SnaBI SspI StuI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccIII 122 TCCGGA T^CCGGA AcsI 1383 GAATTC [GA]^AATT[CT] AflII 1778 CTTAAG C^TTAAG AflIII 3932 ACATGT A^C[GA][CT]GT AhdI 2586 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2037 GTGCAC G^TGCAC ApaLI 2037 GTGCAC G^TGCAC ApoI 1383 GAATTC [GA]^AATT[CT] AseI 3762 ATTAAT AT^TAAT AsnI 3762 ATTAAT AT^TAAT AspEI 2586 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 2065 TGCGCA TGC^GCA BalI 2047 TGGCCA TGG^CCA BciVI 678 GTATCC GTATCC(6/5) BfrI 1778 CTTAAG C^TTAAG BsaBI 1925 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 122 TCCGGA T^CCGGA BspEI 122 TCCGGA T^CCGGA BspHI 3182 TCATGA T^CATGA BspLU11I 3932 ACATGT A^CATGT BsrDI 151 CATTGC GCAATG(2/0) Bst1107I 1811 GTATAC GTA^TAC BstZ17I 1811 GTATAC GTA^TAC CpoI 227 CGGTCCG CG^G[AT]CCG DraIII 3902 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 2586 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 1383 GAATTC G^AATTC FspI 2065 TGCGCA TGC^GCA HincII 1862 GTTGAC GT[CT]^[GA]AC HindII 1862 GTTGAC GT[CT]^[GA]AC MamI 1925 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 2047 TGGCCA TGG^CCA MroI 122 TCCGGA T^CCGGA MscI 2047 TGGCCA TGG^CCA NcoI 682 CCATGG C^CATGG PshAI 2071 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3762 ATTAAT AT^TAAT RcaI 3182 TCATGA T^CATGA RsrII 227 CGGTCCG CG^G[AT]CCG SbfI 2507 CCTGCAGG CCTGCA^GG ScaI 3115 AGTACT AGT^ACT SgrAI 63 CGCCGGTG C[GA]^CCGG[CT]G SmlI 1778 CTTAAG C^T[CT][GA]AG SpeI 3774 ACTAGT A^CTAGT Sse8387I 2507 CCTGCAGG CCTGCA^GG TfiI 3168 GATTC G^A[AT]TC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AccI 1811 GTATAC GT^[AC][GT]AC 1983 GTCTAC AocI 3157 CCTCAGG CC^T[ACGT]AGG 3676 CCTCAGG ApaI 3325 GGGCCC GGGCC^C 3856 GGGCCC BglII 1723 AGATCT A^GATCT 2866 AGATCT BsaAI 1814 TACGTG [CT]AC^GT[GA] 3149 TACGTG Bsp1407I 1633 TGTACA T^GTACA 2977 TGTACA BsrBI 586 CCGCTC CCGCTC(-3/-3) 1542 GAGCGG BsrGI 1633 TGTACA T^GTACA 2977 TGTACA BssHII 79 GCGCGC G^CGCGC 183 GCGCGC Bsu36I 3157 CCTCAGG CC^T[ACGT]AGG 3676 CCTCAGG DrdI 2217 GACAGACTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3284 GACTCCACCGTC Eco81I 3157 CCTCAGG CC^T[ACGT]AGG 3676 CCTCAGG EcoNI 811 CCTGAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2240 CCTGGGAGAGG NheI 46 GCTAGC G^CTAGC 2159 GCTAGC SexAI 2587 ACCTGGT A^CC[AT]GGT 2605 ACCAGGT SspBI 1633 TGTACA T^GTACA 2977 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 136 enzymes) # ######################################### Acc65I AccII AciI AcyI AfaI AluI AlwI AlwNI Asp718I AspHI AvaI AvaII BanI BanII BbeI BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SphI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI