##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AclI AcsI AflII AgeI
AhdI Aor51HI ApoI AscI AseI
AsnI Asp718I AspEI BamHI BciVI
BclI BfrI BlpI Bpu1102I BsaI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspHI BsrGI Bst1107I BstBI BstZ17I
CelII ClaI CpoI DraI DraIII
Eam1105I Eco47III EcoRI EcoRV EcoT22I
FbaI HindIII HpaI KpnI MfeI
MluI MseI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI SwaI Tru9I XbaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 1917 GACGTC GACGT^C
AccIII 1270 TCCGGA T^CCGGA
Asp700I 2961 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1107 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1825 TGCGCA TGC^GCA
AvrII 3885 CCTAGG C^CTAGG
BbrPI 1128 CACGTG CAC^GTG
BbvCI 1134 GCTGAGG CCTCAGC(-5/-2)
BcgI 3122 CGATGTGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 3886 CTAG C^TAG
BlnI 3885 CCTAGG C^CTAGG
BsaAI 1128 CACGTG [CT]AC^GT[GA]
BseAI 1270 TCCGGA T^CCGGA
BspEI 1270 TCCGGA T^CCGGA
BspLU11I 3817 ACATGT A^CATGT
BssHII 1230 GCGCGC G^CGCGC
FseI 3290 GGCCGGCC GGCCGG^CC
FspI 1825 TGCGCA TGC^GCA
HincII 1053 GTCAAC GT[CT]^[GA]AC
HindII 1053 GTCAAC GT[CT]^[GA]AC
KspI 341 CCGCGG CCGC^GG
MaeI 3886 CTAG C^TAG
MroI 1270 TCCGGA T^CCGGA
NaeI 3291 GCCGGC GCC^GGC
NgoMIV 3291 GCCGGC G^CCGGC
PaeR7I 1741 CTCGAG C^TCGAG
PflFI 1107 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1128 CACGTG CAC^GTG
PmlI 1128 CACGTG CAC^GTG
SacII 341 CCGCGG CCGC^GG
SexAI 1108 ACCTGGT A^CC[AT]GGT
SmlI 1741 CTCGAG C^T[CT][GA]AG
Tsp509I 726 AATT ^AATT
Tth111I 1107 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1741 CTCGAG C^TCGAG
XmnI 2961 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 1104 GTAGAC GT^[AC][GT]AC
3063 GTAGAC
Alw44I 420 GTGCAC G^TGCAC
699 GTGCAC
AocI 110 CCTGAGG CC^T[ACGT]AGG
1728 CCTGAGG
ApaLI 420 GTGCAC G^TGCAC
699 GTGCAC
BbeI 169 GGCGCC GGCGC^C
3419 GGCGCC
BglII 823 AGATCT A^GATCT
3933 AGATCT
BsaBI 221 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3440 GATAGACATC
BsaWI 487 TCCGGT [AT]^CCGG[AT]
1270 TCCGGA
BsrDI 557 CATTGC GCAATG(2/0)
1367 CATTGC
BssSI 621 CACGAG CACGAG(-5/-1)
1819 CTCGTG
BstAPI 1466 GCAGAATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2157 GCAGGAAGTGC
BstEII 1211 GGTCACC G^GT[ACGT]ACC
3524 GGTGACC
BstPI 1211 GGTCACC G^GT[ACGT]ACC
3524 GGTGACC
Bsu36I 110 CCTGAGG CC^T[ACGT]AGG
1728 CCTGAGG
DrdI 1656 GACCCCGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2046 GACAACGTGGTC
Eco81I 110 CCTGAGG CC^T[ACGT]AGG
1728 CCTGAGG
EcoNI 608 CCTGCATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1163 CCTGCGGCAGG
EcoO65I 1211 GGTCACC G^GT[ACGT]ACC
3524 GGTGACC
KasI 169 GGCGCC G^GCGCC
3419 GGCGCC
MamI 221 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3440 GATAGACATC
NarI 169 GGCGCC GG^CGCC
3419 GGCGCC
NspI 2134 ACATGC [GA]CATG^[CT]
3817 ACATGT
SfoI 169 GGCGCC GGC^GCC
3419 GGCGCC
XcmI 2131 CCAACATGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2770 CCAACCAGATGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BglI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I SbfI
ScaI ScrFI SfaNI SfcI SfiI
SmaI Sse8387I StuI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I
Van91I XhoII XmaCI XmaI