##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AcsI AflII AgeI AhdI Aor51HI
ApoI AscI AseI AsnI Asp700I
AspEI BcgI BciVI BclI BfrI
BsiWI BspDI BspLU11I BssSI BstBI
ClaI CpoI DraI Eam1105I Eco47III
EcoRI EcoT22I FbaI FseI HindIII
HpaI MfeI MluI MunI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshBI PvuI
RsrII SalI SbfI ScaI SfiI
SfuI SgrAI SnaBI Sse8387I SwaI
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AatII 1434 GACGTC GACGT^C
AccI 3613 GTATAC GT^[AC][GT]AC
AccIII 109 TCCGGA T^CCGGA
AclI 665 AACGTT AA^CGTT
AflIII 1491 ACACGT A^C[GA][CT]GT
AspI 2487 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3464 CCTAGG C^CTAGG
BbeI 2907 GGCGCC GGCGC^C
BbsI 3410 GTCTTC GAAGAC(2/6)
BglII 3808 AGATCT A^GATCT
BlnI 3464 CCTAGG C^CTAGG
BpuAI 3410 GTCTTC GAAGAC(2/6)
BsaBI 1326 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1626 GAGACC GGTCTC(1/5)
BsaWI 109 TCCGGA [AT]^CCGG[AT]
BseAI 109 TCCGGA T^CCGGA
Bsp1407I 2928 TGTACA T^GTACA
BspEI 109 TCCGGA T^CCGGA
BspHI 1205 TCATGA T^CATGA
BspMI 2671 ACCTGC ACCTGC(4/8)
BsrBI 1383 GAGCGG CCGCTC(-3/-3)
BsrDI 820 GCAATG GCAATG(2/0)
BsrFI 75 GCCGGC [GA]^CCGG[CT]
BsrGI 2928 TGTACA T^GTACA
BssHII 699 GCGCGC G^CGCGC
Bst1107I 3613 GTATAC GTA^TAC
BstEII 2099 GGTCACC G^GT[ACGT]ACC
BstPI 2099 GGTCACC G^GT[ACGT]ACC
BstZ17I 3613 GTATAC GTA^TAC
Cfr10I 75 GCCGGC [GA]^CCGG[CT]
DrdI 2194 GACCACATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2099 GGTCACC G^GT[ACGT]ACC
EcoRV 2107 GATATC GAT^ATC
HincII 1548 GTCAAC GT[CT]^[GA]AC
HindII 1548 GTCAAC GT[CT]^[GA]AC
KasI 2907 GGCGCC G^GCGCC
KspI 227 CCGCGG CCGC^GG
MamI 1326 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 109 TCCGGA T^CCGGA
NaeI 75 GCCGGC GCC^GGC
NarI 2907 GGCGCC GG^CGCC
NgoMIV 75 GCCGGC G^CCGGC
NheI 158 GCTAGC G^CTAGC
PflFI 2487 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2746 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 665 AACGTT AA^CGTT
RcaI 1205 TCATGA T^CATGA
SacII 227 CCGCGG CCGC^GG
SapI 2688 GAAGAGC GCTCTTC(1/4)
SfoI 2907 GGCGCC GGC^GCC
SmaI 2379 CCCGGG CCC^GGG
SpeI 3406 ACTAGT A^CTAGT
SspBI 2928 TGTACA T^GTACA
SspI 3492 AATATT AAT^ATT
Tsp509I 4245 AATT ^AATT
Tth111I 2487 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2379 CCCGGG C^CCGGG
XmaI 2379 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 1434 GACGTC G[GA]^CG[CT]C
2907 GGCGCC
Alw44I 2028 GTGCAC G^TGCAC
3002 GTGCAC
AocI 3701 CCTAAGG CC^T[ACGT]AGG
3741 CCTGAGG
ApaI 3083 GGGCCC GGGCC^C
3932 GGGCCC
ApaLI 2028 GTGCAC G^TGCAC
3002 GTGCAC
AvaI 922 CCCGAG C^[CT]CG[GA]G
2379 CCCGGG
AviII 619 TGCGCA TGC^GCA
817 TGCGCA
BamHI 264 GGATCC G^GATCC
1325 GGATCC
BbrPI 2945 CACGTG CAC^GTG
3005 CACGTG
BbvCI 3695 CCTCAGC CCTCAGC(-5/-2)
4233 CCTCAGC
BsaHI 1434 GACGTC G[GA]^CG[CT]C
2907 GGCGCC
BsgI 417 GTGCAG GTGCAG(16/14)
2832 GTGCAG
BsiEI 1368 CGGCCG CG[GA][CT]^CG
1579 CGGCCG
BsoBI 922 CCCGAG C^[CT]CG[GA]G
2379 CCCGGG
BstAPI 731 GCAAGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2501 GCAGATGCTGC
Bsu36I 3701 CCTAAGG CC^T[ACGT]AGG
3741 CCTGAGG
DraIII 1889 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
3448 CACTGTGTG
EagI 1368 CGGCCG C^GGCCG
1579 CGGCCG
EclXI 1368 CGGCCG C^GGCCG
1579 CGGCCG
Eco52I 1368 CGGCCG C^GGCCG
1579 CGGCCG
Eco81I 3701 CCTAAGG CC^T[ACGT]AGG
3741 CCTGAGG
FspI 619 TGCGCA TGC^GCA
817 TGCGCA
HgaI 521 GACGC GACGC(5/10)
3422 GCGTC
Hin1I 1434 GACGTC G[GA]^CG[CT]C
2907 GGCGCC
NcoI 1810 CCATGG C^CATGG
2705 CCATGG
PmaCI 2945 CACGTG CAC^GTG
3005 CACGTG
PmlI 2945 CACGTG CAC^GTG
3005 CACGTG
SexAI 1874 ACCTGGT A^CC[AT]GGT
2980 ACCAGGT
SphI 1020 GCATGC GCATG^C
1237 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
Acc65I AccII AciI AfaI AluI
AlwI AlwNI Asp718I AspHI AvaII
BalI BanI BanII BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I TspRI TthHB8I
Van91I XcmI XhoII