##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AscI AseI AsnI Asp700I AspI
BamHI BbrPI BciVI BclI BfrI
BsaAI BsaBI BsaWI BseAI BsmI
BspDI BspEI BspHI BspLU11I BsrDI
BssSI Bst1107I BstEII BstPI BstZ17I
ClaI CpoI DraI EcoO65I EcoRV
EcoT22I FbaI HincII HindII HpaI
MamI MfeI MluI MroI MunI
NdeI NotI NruI NsiI PacI
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I RcaI RsrII SalI
SapI ScaI SexAI SnaBI SpeI
SspI SwaI TfiI Tth111I XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AcsI 2741 GAATTC [GA]^AATT[CT]
AflIII 4105 ACGTGT A^C[GA][CT]GT
AhdI 3691 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1717 GTGCAC G^TGCAC
Aor51HI 1245 AGCGCT AGC^GCT
ApaLI 1717 GTGCAC G^TGCAC
ApoI 2741 GAATTC [GA]^AATT[CT]
AspEI 3691 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 98 CCTAGG C^CTAGG
BglII 2397 AGATCT A^GATCT
BlnI 98 CCTAGG C^CTAGG
BsiWI 110 CGTACG C^GTACG
BssHII 3593 GCGCGC G^CGCGC
BstBI 332 TTCGAA TT^CGAA
DrdI 1625 GACAGCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1667 CGGCCG C^GGCCG
Eam1105I 3691 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1667 CGGCCG C^GGCCG
Eco47III 1245 AGCGCT AGC^GCT
Eco52I 1667 CGGCCG C^GGCCG
EcoRI 2741 GAATTC G^AATTC
FseI 2084 GGCCGGCC GGCCGG^CC
MseI 4215 TTAA T^TAA
NheI 2231 GCTAGC G^CTAGC
NspV 332 TTCGAA TT^CGAA
PaeR7I 3602 CTCGAG C^TCGAG
SbfI 3100 CCTGCAGG CCTGCA^GG
SfiI 123 GGCCCGGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 332 TTCGAA TT^CGAA
Sse8387I 3100 CCTGCAGG CCTGCA^GG
Tru9I 4215 TTAA T^TAA
XcmI 2097 CCACCTCCCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3602 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 2760 GGTACC G^GTACC
3096 GGTACC
AocI 3307 CCTGAGG CC^T[ACGT]AGG
4144 CCTGAGG
Asp718I 2760 GGTACC G^GTACC
3096 GGTACC
BalI 2835 TGGCCA TGG^CCA
2862 TGGCCA
BbeI 12 GGCGCC GGCGC^C
3556 GGCGCC
BbvCI 3707 CCTCAGC CCTCAGC(-5/-2)
3584 GCTGAGG
BcgI 3776 CGAAGGCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1338 GCAAGGCCATCG
BlpI 2927 GCTGAGC GC^T[ACGT]AGC
4130 GCTTAGC
BpmI 1993 CTGGAG CTGGAG(16/14)
3476 CTGGAG
Bpu1102I 2927 GCTGAGC GC^T[ACGT]AGC
4130 GCTTAGC
Bsu36I 3307 CCTGAGG CC^T[ACGT]AGG
4144 CCTGAGG
CelII 2927 GCTGAGC GC^T[ACGT]AGC
4130 GCTTAGC
DraIII 2499 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
2540 CACCCCGTG
EarI 617 GAAGAG CTCTTC(1/4)
4000 GAAGAG
Eco81I 3307 CCTGAGG CC^T[ACGT]AGG
4144 CCTGAGG
HindIII 3608 AAGCTT A^AGCTT
3763 AAGCTT
KasI 12 GGCGCC G^GCGCC
3556 GGCGCC
KpnI 2760 GGTACC GGTAC^C
3096 GGTACC
Ksp632I 617 GAAGAG CTCTTC(1/4)
4000 GAAGAG
KspI 84 CCGCGG CCGC^GG
3482 CCGCGG
MluNI 2835 TGGCCA TGG^CCA
2862 TGGCCA
MscI 2835 TGGCCA TGG^CCA
2862 TGGCCA
NaeI 2085 GCCGGC GCC^GGC
3621 GCCGGC
NarI 12 GGCGCC GG^CGCC
3556 GGCGCC
NgoMIV 2085 GCCGGC G^CCGGC
3621 GCCGGC
PshAI 3419 GACCGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3670 GACCACTGTC
PvuI 3616 CGATCG CGAT^CG
3908 CGATCG
SacI 484 GAGCTC GAGCT^C
1280 GAGCTC
SacII 84 CCGCGG CCGC^GG
3482 CCGCGG
SfoI 12 GGCGCC GGC^GCC
3556 GGCGCC
SgrAI 8 CACCGGCG C[GA]^CCGG[CT]G
2845 CACCGGCG
SphI 1689 GCATGC GCATG^C
3576 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII AviII BanI BanII
BbsI BbvI BcnI BfaI BglI
BmyI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrBI BsrFI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SspBI
StuI StyI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmaCI XmaI