################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AatII AccIII AclI AflII AgeI AscI AseI AsnI Asp700I AspI BamHI BbrPI BciVI BclI BfrI BsaAI BsaBI BsaWI BseAI BsmI BspDI BspEI BspHI BspLU11I BsrDI BssSI Bst1107I BstEII BstPI BstZ17I ClaI CpoI DraI EcoO65I EcoRV EcoT22I FbaI HincII HindII HpaI MamI MfeI MluI MroI MunI NdeI NotI NruI NsiI PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I RcaI RsrII SalI SapI ScaI SexAI SnaBI SpeI SspI SwaI TfiI Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AcsI 2741 GAATTC [GA]^AATT[CT] AflIII 4105 ACGTGT A^C[GA][CT]GT AhdI 3691 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1717 GTGCAC G^TGCAC Aor51HI 1245 AGCGCT AGC^GCT ApaLI 1717 GTGCAC G^TGCAC ApoI 2741 GAATTC [GA]^AATT[CT] AspEI 3691 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 98 CCTAGG C^CTAGG BglII 2397 AGATCT A^GATCT BlnI 98 CCTAGG C^CTAGG BsiWI 110 CGTACG C^GTACG BssHII 3593 GCGCGC G^CGCGC BstBI 332 TTCGAA TT^CGAA DrdI 1625 GACAGCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1667 CGGCCG C^GGCCG Eam1105I 3691 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1667 CGGCCG C^GGCCG Eco47III 1245 AGCGCT AGC^GCT Eco52I 1667 CGGCCG C^GGCCG EcoRI 2741 GAATTC G^AATTC FseI 2084 GGCCGGCC GGCCGG^CC MseI 4215 TTAA T^TAA NheI 2231 GCTAGC G^CTAGC NspV 332 TTCGAA TT^CGAA PaeR7I 3602 CTCGAG C^TCGAG SbfI 3100 CCTGCAGG CCTGCA^GG SfiI 123 GGCCCGGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 332 TTCGAA TT^CGAA Sse8387I 3100 CCTGCAGG CCTGCA^GG Tru9I 4215 TTAA T^TAA XcmI 2097 CCACCTCCCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 3602 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### Acc65I 2760 GGTACC G^GTACC 3096 GGTACC AocI 3307 CCTGAGG CC^T[ACGT]AGG 4144 CCTGAGG Asp718I 2760 GGTACC G^GTACC 3096 GGTACC BalI 2835 TGGCCA TGG^CCA 2862 TGGCCA BbeI 12 GGCGCC GGCGC^C 3556 GGCGCC BbvCI 3707 CCTCAGC CCTCAGC(-5/-2) 3584 GCTGAGG BcgI 3776 CGAAGGCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1338 GCAAGGCCATCG BlpI 2927 GCTGAGC GC^T[ACGT]AGC 4130 GCTTAGC BpmI 1993 CTGGAG CTGGAG(16/14) 3476 CTGGAG Bpu1102I 2927 GCTGAGC GC^T[ACGT]AGC 4130 GCTTAGC Bsu36I 3307 CCTGAGG CC^T[ACGT]AGG 4144 CCTGAGG CelII 2927 GCTGAGC GC^T[ACGT]AGC 4130 GCTTAGC DraIII 2499 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG 2540 CACCCCGTG EarI 617 GAAGAG CTCTTC(1/4) 4000 GAAGAG Eco81I 3307 CCTGAGG CC^T[ACGT]AGG 4144 CCTGAGG HindIII 3608 AAGCTT A^AGCTT 3763 AAGCTT KasI 12 GGCGCC G^GCGCC 3556 GGCGCC KpnI 2760 GGTACC GGTAC^C 3096 GGTACC Ksp632I 617 GAAGAG CTCTTC(1/4) 4000 GAAGAG KspI 84 CCGCGG CCGC^GG 3482 CCGCGG MluNI 2835 TGGCCA TGG^CCA 2862 TGGCCA MscI 2835 TGGCCA TGG^CCA 2862 TGGCCA NaeI 2085 GCCGGC GCC^GGC 3621 GCCGGC NarI 12 GGCGCC GG^CGCC 3556 GGCGCC NgoMIV 2085 GCCGGC G^CCGGC 3621 GCCGGC PshAI 3419 GACCGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3670 GACCACTGTC PvuI 3616 CGATCG CGAT^CG 3908 CGATCG SacI 484 GAGCTC GAGCT^C 1280 GAGCTC SacII 84 CCGCGG CCGC^GG 3482 CCGCGG SfoI 12 GGCGCC GGC^GCC 3556 GGCGCC SgrAI 8 CACCGGCG C[GA]^CCGG[CT]G 2845 CACCGGCG SphI 1689 GCATGC GCATG^C 3576 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII AviII BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrBI BsrFI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SspBI StuI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmaCI XmaI